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Showing: 6321 - 6360 of 8622 results

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pEGFP-N1_MeCP2(WT)

Plasmid

#110186

Purpose

Expression of mouse MeCP2 (WT) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
hUbc13

Plasmid

#51131

Purpose

human ubc13, E2 for synthesis k63 linked ubiquitin chain

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

ubc13 (UBE2N Human)

Use

Tags

His6 and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-AKAR4

Plasmid

#61619

Purpose

FRET-based biosensor for monitoring PKA activity

Depositor

Jin Zhang

Article

Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

Insert

AKAR4

Use

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMW#3

Plasmid

#13350

Depositor

Marian Walhout

Article

Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

Insert

LacZ reporter

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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CXCR4-Tango

Plasmid

#66262

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CXCR4 (CXCR4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his

Plasmid

#24055

Depositor

Jonathan Backer

Article

Yan et al Biochem J. 2009 Feb 1. 417(3):747-55.

Insert

PIK3C3 and PIK3R4 (PIK3C3, PIK3R4 Human)

Use

Bicistronic

Tags

Myc (hVps34) and V5/his (hVps15)

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed2-pBAD

Plasmid

#54608

Purpose

Localization: Protein Expression Vector , Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

6xHis and DsRed2

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pKIKOlacZKm

Plasmid

#46767

Purpose

E.coli genomic integration at lacZ

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf3b-IRES-Puro

Plasmid

#141384

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3b

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

Plasmid

#141387

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRK7-HA-S6K1-E389-deltaCT

Plasmid

#8993

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 Rheb1 shRNA #1

Plasmid

#26625

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
Tubp-Gal80 in pCaSpeR4

Plasmid

#17748

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL80 (GAL80 Budding Yeast)

Use

Tags

SV40 polyA and Tubulin 1alpha promoter

Expression

Insect

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5-myc-Cdc42-Q61L

Plasmid

#12974

Depositor

Gary Bokoch

Insert

Cdc42 constitutively active (CDC42 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Q61L Constitutively active

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ING2(pHD)

Plasmid

#21589

Depositor

Junying Yuan

Article

Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

Insert

GFP-ING2(pHD) (Ing2 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains Ing2 plant homeodomain (PHD) amino acids…

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pBB572

Plasmid

#27397

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 . 7():32.

Insert

ibpA, ibpB (ibpB, ibpA E.coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-mE1

Plasmid

#32534

Depositor

Jorge Eduardo Azevedo

Article

Carvalho et al Mol Biotechnol. 2011 Oct 20.

Insert

E1, ubiquitin-activating enzyme (Uba1 Mouse)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 Stu2 1-590

Plasmid

#38314

Depositor

Anthony Hyman

Article

Widlund et al Mol Biol Cell. 2012 Sep 19.

Insert

5' 1770 bp from STU2 ORF (STU2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAKlux1

Plasmid

#14073

Depositor

Attila Karsi

Article

Karsi et al FEMS Microbiol Lett. 2006 Jul. 260(2):21

Insert

Bacterial luciferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Flag-H2A

Plasmid

#63560

Purpose

Expression of human H2A tagged with Flag

Depositor

Titia Sixma

Article

Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

Insert

H2A (H2AC13 Human)

Use

Tags

Flag (MDYKDDDDKVPRIQ)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-DN-hCUL2-FLAG

Plasmid

#15819

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin2 (CUL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N427

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Pomc-pGL3

Plasmid

#17553

Purpose

Luciferase expression under control of Pomc promoter (–646 to +65).

Depositor

Domenico Accili

Article

Kitamura et al Nat Med. 2006 May . 12(5):534-40.

Insert

Pomc promoter (Pomc Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Pomc

Available since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1-FLAG-Synaptojanin 1-145

Plasmid

#22293

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Use

Tags

Flag and GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-C Med12His

Plasmid

#49240

Purpose

expresses human Med12 with His tag in insect cells

Depositor

Dylan Taatjes

Article

Knuesel et al Mol Cell Biol. 2009 Feb;29(3):650-61. do

Insert

Med12 (MED12 Human)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-Traptavidin

Plasmid

#24753

Depositor

Mark Howarth

Article

Chivers et al Nat Methods. 2010 Apr 11. ():.

Insert

Traptavidin

Use

Tags

His-6

Expression

Bacterial

Mutation

Streptavidin with 2 amino acid mutations: S52G an…

Promoter

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-iNOS

Plasmid

#19295

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89

Insert

inducible nitric oxide synthase (Nos2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-dCpf1-BE

Plasmid

#107685

Purpose

Expresses dLbCpf1-BE in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE

Use

Tags

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-ABL

Plasmid

#31284

Depositor

Nora Heisterkamp

Article

Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

Insert

V-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
JDS246

Plasmid

#43861

Purpose

Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters

Depositor

Keith Joung

Insert

mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag

Use

CRISPR

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Article

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV5B-TGFbeta receptor II wt

Plasmid

#11766

Depositor

Jeff Wrana, Joan Massague

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSLIK-GFP

Plasmid

#66844

Purpose

Tet-inducible expression of GFP in mammalian cells

Depositor

Christian Metallo

Article

Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFNF

Plasmid

#22687

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Neo*

Use

Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

FRT-pPGK-pEM7-Neo*-bpA-FRT

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pmRFP-N1 human cofilin WT

Plasmid

#50856

Purpose

expresses human cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKNOCK-Cm

Plasmid

#46261

Purpose

Bacterial targeting

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

Type

Empty backbone

Use

Bacterial targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pENTR4 no ccDB (686-1)

Plasmid

#17424

Purpose

Gateway entry vector, no ccDB gene

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab9 WT

Plasmid

#12677

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PDGFRA

Plasmid

#23892

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway donor vector

Tags

Expression

Mutation

M260I

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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