19,186 results

1. tet_pLKO.1_puro_shNRF2 #1

   Plasmid

   #136584

   Purpose

   Expresses an inducible short hairpin targeting human NRF2 sequence

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   shNRF2v1 (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

   Plasmid

   #163029

   Purpose

   FLiCRE TF for neuronal expression

   Depositor

   Alice Ting

   Article

   Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

   Insert

   Nrxn3b-MKII-hLOV1-TEVcs-tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR207 SARS-CoV-2 NSP3

   Plasmid

   #141257

   Purpose

   Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pACEBac1-PARP2-Flag-His-WT

   Plasmid

   #111574

   Purpose

   Expresses full length PARP2 in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP2 (PARP2 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SHP2 WT

   Plasmid

   #8381

   Depositor

   Ben Neel

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pChuA

   Plasmid

   #42539

   Depositor

   Alan Jasanoff

   Article

   Lelyveld et al J Am Chem Soc. 2011 Feb 2;133(4):649-51.

   Insert

   ChuA (chuA E. coli O157:H7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-Golgi eGFP

   Plasmid

   #79809

   Purpose

   To express and target GFP into the Golgi apparatus using lentiviruses

   Depositor

   Pantelis Tsoulfas

   Insert

   Golgi eGFP (B4GALT1 Human)
   

   Use

   Lentiviral

   Tags

   Fusion of the first 62 amino acids of the human b…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-ABEmax-NRTH

   Plasmid

   #136922

   Purpose

   Expresses ABEmax-NRTH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   ABEmax-NRTH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. C5V

   Plasmid

   #26394

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Insert

   Cerulean-5-Venus
   

   Use

   Fret standard

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LeGO-lnc

    Plasmid

    #80624

    Purpose

    Lentiviral lncRNA Overexpression

    Depositor

    Jan-Henning Klusmann

    Article

    Emmrich et al Mol Cancer. 2014 Jul 15;13:171. doi: 10.

    Insert

    empty control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET15b His6-FKBPF36V

    Plasmid

    #73180

    Purpose

    Expressing FKBPF36V protein in washing out shield-1 ligand for FKBPdd

    Depositor

    Thomas Wandless

    Article

    Egeler et al J Biol Chem. 2011 Sep 9;286(36):31328-36

    Insert

    FKBP(F36V) (FKBP1A Human)
    

    Use

    Tags

    His 6x

    Expression

    Bacterial

    Mutation

    F36V

    Promoter

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

12. p3XFlag-CAPN1

    Plasmid

    #60941

    Purpose

    Expresses calpain1 in mammalian cells

    Depositor

    Yi Zhang

    Article

    Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

    Insert

    Calpain1 (CAPN1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

    Plasmid

    #141370

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3.1_sfCherry2(1-10)

    Plasmid

    #82602

    Purpose

    Expresses sfCherry2(1-10) fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glu 118 to Gln, changed Thr 128 to Ile

    Promoter

    CMV

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FUW TetO HA-AMPKa1 WT

    Plasmid

    #69824

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC-VIOL

    Plasmid

    #53087

    Purpose

    Produces the carotenoid violaxanthin in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

    Insert

    zep (ABA1 Mustard Weed)
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Lacks codons for the first 60 N-terminal amino ac…

    Promoter

    T7

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lac-I-SceI-Tet

    Plasmid

    #17655

    Depositor

    Tom Misteli

    Article

    Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

    Insert

    lac I-SceI tet
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 30, 2008

    Availability

    Academic Institutions and Nonprofits only

18. ∆DUSP6

    Plasmid

    #27977

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and MYC

    Expression

    Mammalian

    Mutation

    C293S: mutation in the phosphatase domain

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

19. Lox-stop-Lox p53 R172H

    Plasmid

    #14854

    Depositor

    Tyler Jacks

    Article

    Olive et al Cell. 2004 Dec 17. 119(6):847-60.

    Insert

    p53 R172H (Trp53 Mouse)
    

    Use

    Cre/Lox

    Tags

    Lox-STOP-lox

    Expression

    Mammalian

    Mutation

    Structural mutant R172H. (Murine p53 codon 172 co…

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-mhYFP

    Plasmid

    #186533

    Purpose

    Monomeric hyperfolder YFP fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRubiG-T2A-Cas9

    Plasmid

    #75348

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS6-YFP-SMASh(TI)

    Plasmid

    #111501

    Purpose

    Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    YFP-SMASh(TI)
    

    Use

    Tags

    HA epitope tag and SMASh tag

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, S122R, a…

    Promoter

    CMV

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-MBP-2xNLS-tdTomato

    Plasmid

    #104054

    Purpose

    An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-tdTomato
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    MBP

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LentiV_Neo_LKB1

    Plasmid

    #108111

    Purpose

    Lentiviral expression plasmid of human LKB1 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    LKB1 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223_NOTCH2_WT

    Plasmid

    #82292

    Purpose

    Gateway Donor vector containing NOTCH2, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    NOTCH2 (NOTCH2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    C946Y

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. human K19

    Plasmid

    #14744

    Depositor

    M Bishr Omary

    Article

    Zhou et al J Biol Chem. 1999 Apr 30. 274(18):12861-

    Insert

    Keratin 19 (KRT19 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid

    #111850

    Purpose

    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G12V (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Changed Glycine 12 to Valine

    Promoter

    T5

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-BCL2

    Plasmid

    #116717

    Purpose

    Lentiviral expression of BCL2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BCL2 (BCL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-Syn-ERT2-iCre-ERT2

    Plasmid

    #178059

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin 1 (Syn) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. hGli3 flag3x

    Plasmid

    #84921

    Purpose

    mammalian expression of Gli3

    Depositor

    Martin Fernandez-Zapico

    Insert

    Gli3 (GLI3 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MAC-NRP1

    Plasmid

    #158384

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    NRP1 (NRP1 Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pMRXIP TMEM41B-3xFLAG

    Plasmid

    #117415

    Purpose

    Expresses TMEM41B tagged with 3xFLAG in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    TMEM41B (TMEM41B Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMRXIP Turquoise2-Syntaxin17

    Plasmid

    #89938

    Purpose

    Expresses Syntaxin17 tagged with Turquoise2 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

    Insert

    human Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    mTurquoise2

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONR223_STAT6_WT

    Plasmid

    #81950

    Purpose

    Gateway Donor vector containing STAT6, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFA6a-GFP(S65T)-His3MX6

    Plasmid

    #41598

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1 Hygro EYFP H148Q

    Plasmid

    #25873

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q mutation

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

37. Lv EF1a dCas9-HA 2A Blast

    Plasmid

    #102812

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express dCas9-HA fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    dCas9-HA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A (dCas9)

    Promoter

    EF1a

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid

    #87258

    Purpose

    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

    Depositor

    David Sibley

    Article

    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

    Inserts

    TIR1
    CAT
    

    Use

    Toxoplasma expression

    Tags

    3FLAG

    Expression

    Mutation

    Codon optimized for Toxoplasma gondii expression

    Promoter

    TgSAG1 361 bp and TgTUB1 2717 bp

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRRL_SRSF2_P95H_mCherry

    Plasmid

    #84023

    Purpose

    expression of SRSF2 mutant in mammalian cells

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    2TA-mCherry and Flag

    Expression

    Mammalian

    Mutation

    P95H

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-WT-VWF

    Plasmid

    #124794

    Purpose

    Expresses multimeric human VWF with C-terminal c-Myc tag

    Depositor

    Sriram Neelamegham

    Article

    Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

    Insert

    VWF (VWF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

    Plasmid

    #67985

    Purpose

    Cas9 activity reporter (control) with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pINDUCER20-α-Syn

    Plasmid

    #92200

    Purpose

    Retroviral overexpression vector (doxycycline-inducible) for α-Syn

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    SNCA (SNCA Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

    Plasmid

    #86233

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. PEA1-Puro

    Plasmid

    #171991

    Purpose

    Delivers all prime editing nickase components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pRK5-HA-Ubiquitin-K29R

    Plasmid

    #17602

    Purpose

    Mammalian expression of HA tagged ubiquitin K29R

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29R; V5 deleted

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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46. pGEX-4T-3-mR7BD

    Plasmid

    #79149

    Purpose

    bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

    Depositor

    Aimee Edinger

    Article

    Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

    Insert

    R7BD (Rilp Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 220-299

    Promoter

    tac

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCI-Neo-PTPRT (WT)

    Plasmid

    #16630

    Depositor

    Bert Vogelstein

    Article

    Wang et al Science. 2004 May 21. 304(5674):1164-6.

    Insert

    PTPRT (PTPRT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

48. pLenti.PGK.chFP.W

    Plasmid

    #51008

    Purpose

    Lentivector that expresses chFP from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    mChFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMOD_B2303

    Plasmid

    #91068

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMM114

    Plasmid

    #127211

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, IPT, sgRNA, Luc
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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