162,408 results
-
Plasmid#17293Purpose3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, PuroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only
-
pB-CMPE-ePPEplus
Plasmid#205246PurposeFor plant prime editing in wheat plantsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE
Plasmid#203842PurposeFlp-dependent Cre and EGFP expressionDepositorInsertCre
UseAAVTagsEGFP-2AExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-LaminB1-10
Plasmid#57141PurposeLocalization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmPA-GFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTRIP-SFFV-mtagBFP-2A RIGI WT
Plasmid#167289PurposeLentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-IDepositorInsertDDX58 (RIGI Human)
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp12 (SARS-CoV-2)
Plasmid#169188PurposeTo express His-SUMO-nsp12 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp12 (ORF1ab Synthetic, SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBI-p53 R175H/EGFP
Plasmid#16544DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationR175HPromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-tMCK-ER-TurboID
Plasmid#149412PurposeMuscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER-TurboID
UseAAVTagsHA and V5ExpressionMutationPromotertMCKAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF Con/Fon DREADD Gq-mCherry
Plasmid#183532PurposeINTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND FlpDepositorInsertDREADD Gq-mCherry
UseAAVTagsExpressionMutationNonePromoternEFAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt1(WT)
Plasmid#86637PurposeExpresses eGFP-tagged human Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
PLKO.1-UPF1-CDS
Plasmid#136037PurposeUPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)DepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2 STING KO
Plasmid#217445PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human STINGDepositorInsertsgRNA targeting STING (STING1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mArg1 promoter/enhancer -31/-3810
Plasmid#34571DepositorInsertArg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
UseLuciferaseTagsFirefly LuciferaseExpressionMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
UseTagsExpressionBacterialMutationPromoterownAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
FoxO1-ADA-GFP
Plasmid#35640DepositorInsertFoxO1-ADA-GFP (Foxo1 Mouse)
UseTagsFlag, GFP, and MycExpressionMammalianMutationT24A, S253D and S316A mutationsPromoterCMVAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29
Plasmid#22903DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40
Plasmid#100852PurposeAAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoterDepositorHas ServiceAAV1InsertjRGECO1a
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEEV2/11
Plasmid#166888PurposeHelper/packaging plasmid for production of AAV2/11 pseudotype vectorDepositorInsertAAV2 rep/AAV11 cap
UseAAVTagsExpressionMutationPromoterAAV2 p5Available sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2
Plasmid#203228PurposeCo-expressing membrane-localized Voltron2 and membrane-localized CheRiff.DepositorInsertMembrane-localized optopatch for neuronal dendrites
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-rev-ChR2-tdtomato
Plasmid#18917PurposeCre-dependent viral expression of ChR2-tdTomato for neuronal excitationDepositorInsertChannelrhodopsin 2-tdtomato
UseAAV and Cre/Lox; Adeno-associated virusTagstdtomatoExpressionMutationPromoterCAGAvailable sinceFeb. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_NES_C1
Plasmid#85060PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2260
Plasmid#141998PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX17 (SOX17 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CLC
Plasmid#193559PurposeExpresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-NRCH
Plasmid#136923PurposeExpresses ABEmax-NRCH in mammalian cellsDepositorInsertABEmax-NRCH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92392Purposeproduces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTADepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Golgi-TurboID
Plasmid#200959PurposeTransiently expresses golgi localized TurboID with HA tagsDepositorInsertGolgi-TurboID
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-IQGAP1
Plasmid#30107DepositorInsertIQGAP1 (IQGAP1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CLASP2 512-650 9xS/A
Plasmid#24410DepositorInsertCLASP2 (512-650) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant that c…PromoterAvailable sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6-210
Plasmid#86919PurposeFluorescent reporter for ER calcium signalingDepositorInsertER-GCaMP6-210
UseLentiviralTagsExpressionMammalianMutationK78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…PromoterCAMKIIAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/CALR wt-FLAG
Plasmid#204532PurposeMammalian expression of human CALR wt-FLAGDepositorInsertCALR wt-FLAG (CALR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-Cerulean-T2A-FKBP-5-ptase
Plasmid#40897DepositorInsertPM-FRB-Cerulean-T2A-FKBP-5-ptase
UseTagsExpressionMutationPromoterAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.1.t1
Plasmid#73547PurposetRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.1.t1
pAzFRS.1.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
CuO-LacI-NLS–SNAPf
Plasmid#227368PurposeLentiviral expression of LacI–SNAPDepositorInsertLacI NLS SNAPf
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH c-Met
Plasmid#86498PurposeMammalian expression of human c-MetDepositorInsertMet (MET Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
Plasmid#221354PurposeMammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa; to be used with Anti-GCN4 [IPI-C11L34] heavy chain (Plasmid 221353) to make the antibody.DepositorInsertAnti-GCN4 [IPI-C11L34] Mouse/Rabbit LC kappa
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
lenti SYN-SVI-DIO-dCas9-VPR
Plasmid#164576PurposeExpresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.DepositorInsertA DIO FLAG-dCas9-VPR cassette containing an SV40 intron
UseCRISPR, Cre/Lox, and LentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
HRE-oUnaG
Plasmid#201712PurposeHypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1aDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-HK2-3xFLAG
Plasmid#163451Purposelentiviral overexpression vector for human HK2DepositorInsertHK2 (HK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
MYST3
Plasmid#25181PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMYST3_13 (KAT6A Human)
UseTagsHisExpressionBacterialMutationcontains catalytic region ( residues 497-780).PromoterAvailable sinceOct. 25, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCB59 pDEST crybb1:mKate2
Plasmid#195986PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEQ176
Plasmid#83943Purposeexpress beta-galactosidase in eukaryotic cellsDepositorInsertCMV enhancer/promoter
UseTagsbeta-galactosidaseExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Grin2b KI
Plasmid#131487PurposeEndogenous tagging of GluN2b: N-terminal (amino acid position: S34)DepositorInsertgRNA and GFP donor (Grin2b Rat)
UseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOpen-Bst HF
Plasmid#165539PurposeHigh fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.DepositorInsertBst HF
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 1, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS U6a gRNA
Plasmid#158961PurposegRNA backbone driven by zebrafish U6a promoterDepositorInsertszf U6a promoter
gRNA backbone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV CMV IGF1-FLAG
Plasmid#175170PurposeLentiviral expression of human Igf1-FLAGDepositorInsertIgf1 (IGF1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF151 pcDNA3.1(+)SOD1WT
Plasmid#26397DepositorInsertSOD1 (SOD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-gfp[LVA]
Plasmid#40170DepositorInsertPromotor probe
UseTagsExpressionMutationPromoterAvailable sinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
TFORF0917
Plasmid#142198PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOA3 (NCOA3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
BS-Met15
Plasmid#69198Purposecomplementation of yeast auxotrophic marker MET15DepositorInsertMET15
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-NLRP3 91-710
Plasmid#75140PurposeExpresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cellsDepositorInsertNLR family, pyrin domain containing 3 (Nlrp3 Mouse)
UseTagsFlagExpressionMammalianMutationfragment encoding amino acids 91-710PromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only