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pcDNA-R-diLACCO1
Plasmid#208028PurposeControl biosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-AcGFP-P2A-hBRD2
Plasmid#197880Purposestudy roles of human BET proteins in human pluripotency reprogrammingDepositorInsertBRD2 (BRD2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2860
Plasmid#143643PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertID1 (ID1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-PLIN3
Plasmid#222426PurposeTo express PLIN3 with mEGFP tag in mammalian cellsDepositorInsertPLIN3 (PLIN3 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Green Glifon50
Plasmid#126206Purposegreen fluorescent glucose sensor for mammalian cellsDepositorInsertGreen Glifon50
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-EGFP
Plasmid#86639PurposeExpresses mCherry-eGFP tandem (cytoplasmic)DepositorInsertmCherry-EGFP
UseTagsmCherry-EGFPExpressionMammalianMutationPromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52546PurposeTargeting vector for homologous recombination in mouse ES cells; Rosa26 locusDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
B-GRAM-W
Plasmid#211707PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cellsDepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
UseTagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to TryptophanPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV297
Plasmid#204820PurposeAMA1 plasmid with amdS selection markerDepositorInsertamdS
UseFungal expressionTagsExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans trpC promoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLOX-Ttag-iresTK
Plasmid#12246DepositorInsertSV40 Large T, HSV1-TK (SV40gp6 Simian virus)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationLoxP in 3' LTR.PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
KA1042_pPB-hCMV*1-Esrrb-IV
Plasmid#124177PurposepiggyBac-based Dox-inducible expression of EsrrbDepositorInsertEsrrb-IRES-Venus (Esrrb Mouse)
UseTagsExpressionMammalianMutationPromotertetO-CMVminAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY088_AAV_EFS-BCMA-41BBz-PRODH2
Plasmid#192191PurposeBCMA CAR AAV vector PRODH2 KI (pLY088)DepositorInsertBCMA CAR AAV vector PRODH2 KI (pLY088) (TNFRSF17 Synthetic)
UseAAV; Mammalian expressionTagsExpressionMutationNAPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-AAK1
Plasmid#23844DepositorInsertAAK1 (AAK1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBB541
Plasmid#27394DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC2A4
Plasmid#132102PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A4 (SLC2A4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1 TEV (full-length)
Plasmid#64276PurposeExpresses tobacco etch virus protease (TEV)DepositorInsertTobacco etch virus (TEV) protease
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
GZnP3 in pcDNA3.1+
Plasmid#161738PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)DepositorInsertGZnP3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-pNFkB::Cre-mRuby3
Plasmid#164137PurposeLentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFaDepositorInsertNFkB promoter-Cre-T2A-mRuby3
UseCRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterNFkB synthetic promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-Irf7
Plasmid#178445Purposedoxycycline-inducible expression of Mouse Irf7 in mammalian cellsDepositorInsertIrf7 (Irf7 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett
Plasmid#205703PurposeCMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell linesDepositorInsertLyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6c:sgRNA#4
Plasmid#64248Purposeexpresses sgRNA under U6c promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
R777-E137 Hs.MTOR
Plasmid#70421PurposeGateway ORF clone of human MTOR [NM_004958.3] with stop codon (for native or N-terminal fusions)DepositorInsertMTOR (MTOR Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
AiP12689 - pAAV-AiE0682h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2689)
Plasmid#191727PurposeAI plasmid ALIAS: AiP12689. Expression of SYFP2 (yellow fluorescent protein) in striatal and neocortical Sst-Chodl neuronsInsertSYFP2
UseAAVTagsExpressionMutationnonePromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAP WT
Plasmid#123097PurposeExpresses 3xFLAG-GABARAP wild-type in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein (GABARAP Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1801_pmax-dCasRx
Plasmid#118634PurposeTransient transfection; Expresses dCasRx; CAGGS promoterDepositorInsertdCasRx
UseTagsExpressionMammalianMutationR239A/H244A/R858A/H863APromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-TO-scHCVP
Plasmid#116088PurposeExpresses an engineered HCV proteaseDepositorInsertscHCVP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST ERKKTRClover
Plasmid#90227PurposeLentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
SSTR4-Tango
Plasmid#66505PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertSSTR4 (SSTR4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pENTR-D-Topo-hTRIM9
Plasmid#51032Purposeentry vector for cloning human TRIM9DepositorInsertTRIM9 (TRIM9 Human)
UseEntry vectorTagsExpressionMutationPromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-EGR1
Plasmid#52724Purposeretroviral expression of human EGR1DepositorInsertEGR1 (EGR1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
PT7-deGFP-MGapt
Plasmid#67741PurposeT7 promoter expressing deGFP-MG aptamer fusionDepositorInsertUTR1-deGFP-MGapt-T7term
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPT7Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF-FH-TAZ S89A-ires-blast
Plasmid#52084PurposeLentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tagsDepositorInsertTAZ S89A (WWTR1 Human)
UseLentiviralTagsFLAG and HisExpressionMammalianMutationS89APromoterEF1aAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
RED
Plasmid#139835PurposeExpresses RNase H1-EGFP- dCas9 fusion protein in human cellsDepositorInsertRNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
UseTagsExpressionMammalianMutationdCas9 protein contains two mutations: D10A and H8…Promotertet-inducible CMVAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE
Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPo (phoenol-inducible promoter)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
ARR3tk-eGFP/SV40-mCherry
Plasmid#132360PurposeTo measure transcriptional activity of Androgen ReceptorDepositorInsertAR responsive elements (AR Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV809
Plasmid#126602Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-Puro-Ak-ACE2-TMPRSS2
Plasmid#154987PurposeLentiviral vector expressing Human ACE2 and TMPRSS2DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH1v3 (+TEV)
Plasmid#204229PurposeBacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).InserthMDH1v3 (MDH1 Human)
UseTags6X HisExpressionBacterialMutationInserted a G after ATG to maintain kozak sitePromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX317-QKI
Plasmid#115441PurposeConstitutive lentiviral expressionDepositorInsertQKI (QKI Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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TFORF0542
Plasmid#143412PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFAT (ZFAT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6promoterEGFP
Plasmid#112896PurposeTo monitor the activation of the murine IL-6 promoter by eGFP expressionDepositorInsertmurine IL-6 promoter (Il6 Mouse)
UseTagsExpressionMammalianMutationPromoterIL-6 promoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ps-CFP
Plasmid#13034PurposeExpresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescenceDepositorInsertphoto-switchable cyan fluorescent protein
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2 Chfr (1014)
Plasmid#61853Purposemammalian expresion of Chfr fused to EGFPDepositorInsertChfr (CHFR Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFR L858R
Plasmid#11012PurposeRetroviral construct for expressing human EGFR with L858R mutation in human cellsDepositorInsertEGFR L858R (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL858R (tumor-derived)PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-PercevalHR
Plasmid#49081PurposeBacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.DepositorInsertPercevalHR
UseTags7xHisExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE-zCas9
Plasmid#101739Purposeexpression of an optimized Cas9 for base-editing in zebrafishDepositorInsertrAPOBEC1-XTEN-zCas9-UGI-NLS
UseCRISPRTagsExpressionMammalianMutationD10APromoterCMVAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only
