8,964 results

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pDS257

Plasmid

#34971

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBac1 Flag BAF155 b

Plasmid

#1954

Depositor

Robert Kingston

Article

Phelan et al Mol Cell 1999 Feb;3(2):247-53.

Insert

BAF155 (SMARCC1 Human)

Tags

flag

Expression

Insect

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pCMV5-ALK2 wt

Plasmid

#11741

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

ALK2 (ACVR1 Human)

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pLCRcala (p53)

Plasmid

#12136

Depositor

James Sherley

Article

Sherley et al J Biol Chem. 1991 Dec 25. 266(36):24815-

Insert

p53 (Trp53 Mouse)

Expression

Mammalian

Available Since

Sept. 21, 2006

Availability

Academic Institutions and Nonprofits only
pLV-tetO-Sox2

Plasmid

#19765

Depositor

Konrad Hochedlinger

Article

Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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Myr-(iSH2-p85)-p110alpha-Myc

Plasmid

#1410

Depositor

Lewis Cantley

Insert

PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

SRalpha promoter

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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p133 pSV-STOP-luc

Plasmid

#8390

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed STOP

Use

Cre/Lox and Luciferase

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn-FLEX.iGABASnFR.F102G

Plasmid

#112164

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Has Service

AAV1

Insert

iGABASnFR.F102G

Use

AAV

Mutation

F102G

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC157-20XUAS-IVS-B3::PEST

Plasmid

#32136

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3::PEST

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-NpHR3.3-EYFP

Plasmid

#137154

Purpose

Intersectional viral expression of NpHR3.3-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-NpHR3.3-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

W179F

Promoter

nEF

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PIK3CB

Plasmid

#20573

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIK3CB (PIK3CB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Expression

Worm

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 tdAPX W41F

Plasmid

#40863

Purpose

Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

tandem dimer pea APX W41F

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pWS-TK6

Plasmid

#20350

Depositor

Mario Capecchi

Article

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Type

Empty backbone

Use

Mouse Targeting

Available Since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

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pEX-HcRed-hLC3WT

Plasmid

#24991

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with HcRed (MAP1LC3B Human)

Tags

HcRed

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-5aHA

Plasmid

#18032

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5a (Wnt5a Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
DAXX-myc-his

Plasmid

#1852

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Tags

his and myc

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
1017 pcDNA3 Myr Akt1 deltaPH

Plasmid

#9009

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 dPH (AKT1 Human)

Tags

myr (MGSSKSKPKDPSQR)

Expression

Mammalian

Mutation

delta PH domain (aa1-130)

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
TRAF6-UTR

Plasmid

#15093

Depositor

David Baltimore

Article

Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

Insert

TRAF6 3'UTR (TRAF6 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 6, 2007

Availability

Academic Institutions and Nonprofits only
pLIA mNIC myc (CC#341)

Plasmid

#15131

Depositor

Connie Cepko

Article

Bao et al J Neurosci. 1997 Feb 15. 17(4):1425-34.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Cytoplasmic domain initiating at amino acid 1753.…

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA

Plasmid

#68719

Purpose

Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6f from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Has Service

AAV1

Insert

mRuby2-P2A-GCaMP6f

Use

AAV

Promoter

CAG-FLEX

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Dppa3

Plasmid

#13365

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 3 (Dppa3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
LZRS-Flag_EZH2_ER

Plasmid

#26111

Depositor

Howard Chang

Article

Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

Insert

EZH2 (EZH2 Human)

Use

Retroviral

Tags

ER and FLAG

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pGAL4-CREB S133A

Plasmid

#15222

Depositor

Ugo Moens

Article

Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Insert

CREB S133A (Creb1 Rat)

Tags

Gal4

Expression

Mammalian

Mutation

Serine 133 mutated to Alanine

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUPER.PKCdelta.RNAi

Plasmid

#10819

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC delta RNAi (PRKCD Human)

Use

RNAi

Expression

Mammalian

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-Coff/Fon-GCaMP6M

Plasmid

#137121

Purpose

Intersectional viral expression of GCaMP6M in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-GCaMP6M

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

Removed RSET tag

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMLM36 (aka pBR-UV5-GP-FD2)

Plasmid

#21871

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pgLAP1

Plasmid

#19702

Depositor

Peter Jackson

Type

Empty backbone

Tags

EGFP, S peptide, and TEV

Expression

Mammalian

Available Since

Feb. 19, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TET-mOct4

Plasmid

#20907

Purpose

piggyBac vector with tet inducible mOct4

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Oct4 (Pou5f1 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP

Plasmid

#137141

Purpose

Intersectional viral expression of ChR2(ET/TC)-EYFP in cells expressing Flp AND NOT Cre

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Coff/Fon-ChR2(ET/TC)-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

E123T, T159C

Promoter

nEF

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-hcMYC (Plath)

Plasmid

#17966

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

v-myc myelocytomatosis viral oncogene homolog (avian) (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

n/a

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKIIa-hChR2(E123A)-mCherry

Plasmid

#35506

Purpose

AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

E123A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST>stop>mCD8-GFP

Plasmid

#24385

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

mCD8-GFP

Expression

Insect

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pAdEasy mCherry-Tubulin

Plasmid

#26767

Depositor

Torsten Wittmann

Article

Matov et al Nat Methods. 2010 Sep . 7(9):761-8.

Insert

Tubulin (TUBA1B Human)

Use

Adenoviral

Tags

mCherry

Expression

Mammalian

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only
pattB-QF-hsp70

Plasmid

#24368

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Expression

Insect

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
LZRS ERTm RasV12

Plasmid

#21199

Depositor

Paul Khavari

Article

Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

Insert

EsR1- HRas (Esr1, HRAS Human, Mouse)

Use

Retroviral

Mutation

Esr1 G257R; HRasG12V ERTm bx3

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
ROSAFARY

Plasmid

#13446

Depositor

Philippe Soriano

Article

Chen et al Nat Genet. 2004 Mar . 36(3):304-12.

Insert

ROSAFARY

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60-Exp5

Plasmid

#12553

Depositor

Ian Macara

Article

Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

Insert

EXPORTIN-5 (XPO5 Human)

Tags

HIS

Expression

Bacterial

Mutation

NONE

Available Since

Oct. 2, 2006

Availability

Academic Institutions and Nonprofits only
Monomer NH_V2

Plasmid

#37527

Purpose

template for PCR amplification of the monomer used for assembly of the DNA-binding tandem repeat region of the TALE.

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

Monomer NH

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pAd 2B Ngn3-I-nGFP

Plasmid

#19410

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

neurogenin 3 (Neurog3 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
HA-FOXO3a WT-ER

Plasmid

#8355

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

mouse estrogen receptor (G525R) on C-term

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd NeuroD-I-nGFP

Plasmid

#19414

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
TV-hPITX3-forward

Plasmid

#22073

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PITX3 (ASMD Human)

Use

Cre/Lox; Zfn donor

Tags

eGFP

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM1.127

Plasmid

#21384

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pie-1 promoter (short, no ATG) in pDONRP4P1R

Use

Gateway Cloning

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMKIIa-hChR2(E123A)-EYFP

Plasmid

#35505

Purpose

AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Has Service

AAV9

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSSK p85 alpha (delta iSH2)

Plasmid

#13432

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta iSH2) (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Mutation

Dominant negative p85 alpha. The p110 alpha bindi…

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
3341 pcDNA3 Bcl-2 T69A S70A S87A

Plasmid

#13340

Depositor

Stanley Korsmeyer

Article

Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

Insert

Bcl-2 (BCL2 Human)

Expression

Mammalian

Mutation

changed Threonine 69 to Alanine; changed Serine 7…

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
PL-452 C-EGFP

Plasmid

#19178

Depositor

Patrik Verstreken

Article

Venken et al Nucleic Acids Res. 2008 Oct;36(18):e114.

Insert

EGFP

Use

Cre/Lox

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
MLM3639

Plasmid

#42252

Purpose

Cas9 expression vector

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Insert

Streptococcus pyogenes Cas9-3X Flag

Use

CRISPR; Zebrafish expression

Tags

3X Flag

Promoter

CMV

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,964 results