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164,425 results

Showing: 9201 - 9250 of 164425 results

  2. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  4. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  9. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

    Plasmid
    #191500
    Purpose
    GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye
    Depositor
    Michael Granato
    Article
    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:
    Insert
    CaSR-EGFP-SV40
    Use
    Creation of transgenic fish using tol2 transgenes…
    Tags
    Expression
    Mutation
    Promoter
    myo6b
    Available Since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    191500-plasmid-map-sequence-id-379300

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  10. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  11. pCali2

    Plasmid
    #86119
    Purpose
    Multiplex temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA36
    mWasabi
    TcI
    mCherry
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T and D341A, P60L, D135V, K187R, K202I, L208Q
    Promoter
    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI
    Available Since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86119-plasmid-map-sequence-id-173197

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  12. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid
    #72325
    Purpose
    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET28a SnoopTag-mEGFP-SpyTag
    Use
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72325-plasmid-map-sequence-id-137259

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  15. pN1-OPA1(123Nt)-mScarletI

    Plasmid
    #129343
    Purpose
    Mitochondrial intermembrane space localization
    Depositor
    Yasushi Okada
    Insert
    mScarlet(i) (Addgene 85044)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    129343-plasmid-map-sequence-id-260106

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  16. pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

    Plasmid
    #204359
    Purpose
    AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter
    Depositor
    Masao Doi
    Article
    Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008
    Insert
    miniDq-mCherry-P2A-HA-KORD
    Use
    AAV
    Tags
    HA (for KORD) and mCherry (for miniDq)
    Expression
    Mutation
    For miniDq, the third intracellular loop (ICL3) o…
    Promoter
    hSyn
    Available Since
    Oct. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204359-plasmid-map-sequence-id-401492

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  21. DRD1-Tango

    Plasmid
    #66268
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    DRD1 (DRD1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66268-plasmid-map-sequence-id-245407

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  22. pCF806-shABC1

    Plasmid
    #186713
    Purpose
    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.
    Depositor
    Christof Fellmann
    Article
    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023
    Insert
    ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    186713-plasmid-map-sequence-id-376087

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  24. pSBtet-Hyg

    Plasmid
    #60508
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and hygromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60508-plasmid-map-sequence-id-97539

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  27. TFORF0912

    Plasmid
    #143449
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF831 (ZNF831 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143449-plasmid-map-sequence-id-397934

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  38. MC5R-Tango

    Plasmid
    #66431
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    MC5R (MC5R Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  41. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid
    #208048
    Purpose
    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    BirAopt
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    BirAopt is human-codon-optimized BirA (PMID 15707…
    Promoter
    EFS
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208048-plasmid-map-sequence-id-410202

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  42. PB-PEmax SpG

    Plasmid
    #187895
    Purpose
    All-in-one prime editor piggyBac transposon with PEmax, SpG variant
    Depositor
    Tobias Cantz
    Article
    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1
    Insert
    SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    BPSV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    187895-plasmid-map-sequence-id-370756

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  45. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

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  47. pRDA_816

    Plasmid
    #201157
    Purpose
    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa
    Depositor
    John Doench
    Article
    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:
    Insert
    5x-ALFA tag-dEnAsCas12a-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D908A
    Promoter
    EFS
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    201157-plasmid-map-sequence-id-396590

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  48. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

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Showing: 9201 - 9250 of 164425 results