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179,521 results

Showing: 7401 - 7440 of 179521 results

  1. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

    Plasmid
    #162378
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    jGCaMP8m
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    A25G F286Y
    Promoter
    Synapsin
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    162378-plasmid-map-sequence-id-318373

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  4. pCX-SpiCee

    Plasmid
    #140836
    Purpose
    mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling
    Depositor
    Xavier Nicol
    Publication
    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpiCee
    Tags
    mRFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140836-plasmid-map-sequence-id-304813

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  5. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Publication
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AIMTOR T757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

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  16. pLX_TRC311

    Plasmid
    #113668
    Purpose
    Gateway destination vector for lentiviral expression
    Depositor
    John Doench
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113668-plasmid-map-sequence-id-220922

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  21. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Yubin Zhou
    Publication
    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LOV2 and STIM1 cytosolic fragment
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101245-plasmid-map-sequence-id-209272

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  22. pET28a-HaloTag7-SnoopLigase

    Plasmid
    #105627
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7
    Depositor
    Mark Howarth
    Publication
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    HaloTag7-SnoopLigase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105627-plasmid-map-sequence-id-207217

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  23. pAAV.GFAP.eGFP.WPRE.hGH

    Plasmid
    #105549
    Purpose
    AAV expression of EGFP from GFAP promoter
    Depositor
    James M. Wilson
    Has service
    AAV5
    Insert
    eGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    GFAP
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105549-plasmid-map-sequence-id-202447

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  31. pFC333

    Plasmid
    #87844
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker
    Depositor
    Uffe Mortensen
    Publication
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    ble (bleomycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87844-plasmid-map-sequence-id-174620

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  39. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid
    #83485
    Purpose
    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).
    Depositor
    Jennifer Doudna
    Publication
    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature
    Insert
    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    Use
    CRISPR
    Tags
    His6-MBP
    Expression
    Bacterial
    Mutation
    R472A, H477A, R1048A, H1053A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83485-plasmid-map-sequence-id-159341

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Showing: 7401 - 7440 of 179521 results