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Showing: 7401 - 7440 of 167150 results

  5. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  11. pAS134

    Plasmid
    #211505
    Purpose
    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    Venus
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Expression
    Mammalian
    Available Since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211505-plasmid-map-sequence-id-417792

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  16. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

    Plasmid
    #166504
    Purpose
    Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.
    Depositor
    Angelos Constantinou
    Article
    Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2
    Insert
    None
    Tags
    TurboID and mCherry-CRY2
    Expression
    Mammalian
    Available Since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166504-plasmid-map-sequence-id-328214

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  17. pCAG-VSVG

    Plasmid
    #64084
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV glycoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64084-plasmid-map-sequence-id-107221

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  23. pCE27

    Plasmid
    #174405
    Purpose
    C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Article
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174405-plasmid-map-sequence-id-349710

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  33. Membrane mCherry

    Plasmid
    #210470
    Purpose
    Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Insert
    LCK membrane mCherry (LCK Human, Synthetic, A.victoria)
    Use
    Synthetic Biology; Sea urchin, mammal, zebrafish
    Tags
    mCherry
    Available Since
    Dec. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    210470-plasmid-map-sequence-id-416828

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  36. pAAV-nEF Con/Foff DREADD Gq-mCherry

    Plasmid
    #183533
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Mutation
    None
    Promoter
    nEF
    Available Since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183533-plasmid-map-sequence-id-362041

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Showing: 7401 - 7440 of 167150 results