Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 31,652 results for: REP

Clear keywords
Showing: 9301 - 9350 of 31652 results

  2. pGL4.R_TRE_minTK

    Plasmid
    #211516
    Purpose
    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression
    Depositor
    Justin English
    Article
    Zahm et al Nat Commun. 2024 Nov 28;15(1):10353. doi
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    SV40 and minTK
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211516-plasmid-map-sequence-id-419246

    Enlarge

  4. pdCRISPomyces-2BD

    Plasmid
    #234429
    Purpose
    Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified dCas9(D10A, H840A) with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234429-plasmid-map-sequence-id-468849

    Enlarge

  5. pKHR8

    Plasmid
    #74625
    Purpose
    a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.
    Depositor
    David Grunwald
    Article
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Inserts
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    cmlc2::mCherry
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    alpha-crystallin and cmlc2
    Available Since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    74625-plasmid-map-sequence-id-200444

    Enlarge

  8. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

    Enlarge

  10. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive native promoters
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65006-plasmid-map-sequence-id-109903

    Enlarge

  15. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Depositor
    Debojyoti Chakraborty
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Promoter
    CBH
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200389-plasmid-map-sequence-id-407168

    Enlarge

  25. pSSA-eGFP (pXK-221)

    Plasmid
    #208826
    Purpose
    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208826-plasmid-map-sequence-id-417242

    Enlarge

  41. pJEC726

    Plasmid
    #190814
    Purpose
    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Article
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Mutation
    S. pyogenes dCas9 codon optimized for Streptomyce…
    Promoter
    SP30
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190814-plasmid-map-sequence-id-373712

    Enlarge

  43. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Calvin Tiengwe
    Article
    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Promoter
    Pol II readthrough transcription after integration
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210224-plasmid-map-sequence-id-415797

    Enlarge

  47. pWR76

    Plasmid
    #202776
    Purpose
    episomal fluorescent reporter plasmid for S. stipitis
    Depositor
    J. Brian Robertson
    Article
    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    202776-plasmid-map-sequence-id-417311

    Enlarge

Showing: 9301 - 9350 of 31652 results