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162,536 results

Showing: 9301 - 9350 of 162536 results

  6. pJYDC1

    Plasmid
    #162458
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA tag, HDEL sequence, eUnaG2 fluorescent protein…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162458-plasmid-map-sequence-id-321474

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  9. MSCV-Cre

    Plasmid
    #24064
    Purpose
    Retroviral coexpression of Cre and EGFP
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    MESV 5' LTR
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24064-plasmid-map-sequence-id-322907

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  15. PLJR965

    Plasmid
    #115163
    Purpose
    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Sarah Fortune
    Article
    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:
    Inserts
    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    115163-plasmid-map-sequence-id-222098

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  16. Epac-S-H74

    Plasmid
    #170338
    Purpose
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:
    Insert
    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    170338-plasmid-map-sequence-id-335510

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  21. pCutamp

    Plasmid
    #140632
    Purpose
    Plasmid-curing in Escherichia coli by targeting the AmpR promoter
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140632-plasmid-map-sequence-id-306006

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  29. AAV-CAG-jGCaMP8f-WPRE

    Plasmid
    #179254
    Purpose
    AAV-mediated expression of GCaMP8f under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179254-plasmid-map-sequence-id-381183

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  30. mKate2-P2A-APEX2-GBP

    Plasmid
    #67650
    Purpose
    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    APEX2-GBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67650-plasmid-map-sequence-id-116990

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  31. pAK_DR30_CasRx_mCherry

    Plasmid
    #134844
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Michael Yaffe
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    134844-plasmid-map-sequence-id-266704

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  32. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  37. pBFC0984

    Plasmid
    #231992
    Purpose
    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site
    Depositor
    Brady Cress
    Article
    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d
    Inserts
    dRfxCas13d
    crRNA with 2xBsaI spacer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 and pTet
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231992-plasmid-map-sequence-id-467618

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  40. OPRM1-DuET

    Plasmid
    #213361
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRM1 (OPRM1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213361-plasmid-map-sequence-id-431826

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  46. TFORF1037

    Plasmid
    #144269
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WT1 (WT1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144269-plasmid-map-sequence-id-404154

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  47. HTR2C-Tango

    Plasmid
    #66411
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR2C (HTR2C Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66411-plasmid-map-sequence-id-139337

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  49. FUGW-d2GFP-200

    Plasmid
    #79602
    Purpose
    FUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sites
    Depositor
    Jeffrey Rosen
    Article
    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.
    Insert
    d2GFP + miR-200 family targets
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    79602-plasmid-map-sequence-id-152507

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Showing: 9301 - 9350 of 162536 results