8,884 results
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pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1B
Plasmid#64221PurposeExpression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherry-P2A-E1BExpressionMammalianPromoterCBh and U6Available SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKIKOlacZKm
Plasmid#46767PurposeE.coli genomic integration at lacZDepositorTypeEmpty backboneUseE.coli genomic integrationAvailable SinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQCXI Neo DsRed-LC3-GFP
Plasmid#31183DepositorInsertDsRed-LC3-GFP
UseRetroviralExpressionMammalianAvailable SinceJune 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIκBα-miRFP703
Plasmid#80005PurposeIκBα fused with near-infrared fluorescent protein miRFP703 (IκBα reporter)DepositorAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-VAPA(1-242)
Plasmid#18874DepositorAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pACAGW-H2B-PAGFP-AAV
Plasmid#33000DepositorInsertH2B-PAGFP (H2BC21 Aequorea victoria, Human)
UseAAV; Adeno associated virusTagsPAGFPExpressionMammalianAvailable SinceDec. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Ub-K63R
Plasmid#18898DepositorInsertUbiquitin-K63R (UBC Human)
ExpressionBacterialMutationLysine 63 is replaced with a Argnine residueAvailable SinceSept. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEMTEZ-TeTxLC
Plasmid#32640DepositorInsertTetanus toxin light chain
ExpressionBacterialAvailable SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTiger-OptoSOS
Plasmid#86439PurposeSingle-construct blue light optogenetic Ras activator for use in DrosophilaDepositorInsertOptoSOS
TagstagRFPExpressionInsectPromoterGal4-UASAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a2SE
Plasmid#49169PurposepHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-2 (GABRA2 Human)
TagsHA, bovine alpha 1 signal sequence, and pHGFP (pH…ExpressionMammalianMutationT343APromoterCMVAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
p3x-FLAG-hnRNPF
Plasmid#21926DepositorAvailable SinceAug. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
408 pSG5L HA E2F1
Plasmid#10736DepositorAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
903 pLNCX HA Akt1
Plasmid#9004DepositorAvailable SinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-D10A
Plasmid#51130PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationD10A (nickase mutant)PromoterCMVAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagB wt
Plasmid#19301DepositorAvailable SinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
hTLR5 flag
Plasmid#13088DepositorInsertTLR5 (TLR5 Human)
TagsFlagExpressionMammalianMutationAmino acids 1-19 have been replaced by a mammalia…Available SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
Venus-pBAD
Plasmid#54859PurposeLocalization: Protein Expression Vector , Excitation: 515, Emission: 528DepositorTypeEmpty backboneTags6xHis and VenusExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA cyclin D1 HA
Plasmid#11181DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Hmga2 wt
Plasmid#14789DepositorInsertHmga2 wt (Hmga2 Mouse)
ExpressionMammalianAvailable SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
YFP-FKBP (YF)
Plasmid#20175DepositorInsertYFP-FKBP (YF): (FKBP1A Human)
ExpressionMammalianMutationFKBP was taken out from pC4M-F2E with a flanking …Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZW16 (CK2alpha')
Plasmid#27087DepositorAvailable SinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
hTLR3 flag
Plasmid#13084DepositorInsertTLR3 (TLR3 Human)
TagsFlagExpressionMammalianMutationAmino acids 1-17 have been replaced by mammalian …Available SinceOct. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Sox10
Plasmid#45843DepositorAvailable SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
aMHC-eGFP-Rex-Neo
Plasmid#21229DepositorInsertalpha myosin heavy chain promoter
UseLentiviralTagseGFPAvailable SinceJune 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-fSST-RFP
Plasmid#22913DepositorInsertfugu somatostatin promoter driving DsRed 2
UseAAVExpressionMammalianAvailable SinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 mouse src
Plasmid#13663DepositorAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pY30
Plasmid#84745PurposeExpresses huAsCpf1-P2A-puro and crRNA guideDepositorInsertshuAsCpf1
puromycin resistance gene
Tags3xHA, NLS, and T2AExpressionMammalianPromoterCMVAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA_GFP-T2
Plasmid#41820PurposeExpresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineeringDepositorInsertgRNA_GFP-T2
UseCRISPRExpressionMammalianAvailable SinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
PBX1A-pCMV1
Plasmid#21029DepositorInsertPBX1 (PBX1 Human)
ExpressionMammalianAvailable SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCerulean-VSVG
Plasmid#11913DepositorInsertVSVG
ExpressionMammalianMutationF64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
Mito-ABKAR
Plasmid#61509PurposeExpression of AMPK biosensor targeted to the mitochondriaDepositorInsertAMPK Biosensor
TagsMitochondrial targeting signal (DAKAP1-30)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
NGFR P210
Plasmid#27486DepositorUseRetroviralTagsIRES and NFGRExpressionMammalianMutationContains the "complete" bcr/abl fusionAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pC.HA Vav1
Plasmid#14553DepositorAvailable SinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagA wt
Plasmid#19298DepositorAvailable SinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct
Plasmid#86230PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.DepositorInsertAID[71-114]-eGFP
UseMouse TargetingExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP15
Plasmid#141268PurposeGateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)
Plasmid#44027DepositorInsertmHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
TagsMycExpressionMammalianMutationP405A, P530V, N851APromoterCMVAvailable SinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 deltaN47 Beta-catenin
Plasmid#19287DepositorInsertbeta-catenin (CTNNB1 Human)
TagsFlagExpressionMammalianMutationN-terminal truncation, deleted aa 1-47Available SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-EOS-C(3+)-EGFP
Plasmid#21318PurposeLentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFPDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralExpressionMammalianPromoterEOS-C(3+) promoter regionAvailable SinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only
