162,536 results
-
-
pEGFP-coilin
Plasmid#36906DepositorInsertcoilin (COIL Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 TaGu N-1F
Plasmid#203762PurposeFor expression of N-flag tagged TaGu R2 protein in mammalian cellsDepositorInsertR2 retroelement protein
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBJ FLAG-hPar1
Plasmid#53226Purposemammalian expression of human Par1 with N-terminal FLAG tagDepositorInsertPar1 (F2R Human)
UseTagsFLAG and bovine prolactin signal sequence (MDSKGS…ExpressionMammalianMutationDeletion of aa1-39 (native signal peptide)PromoterSV40Available sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-IRF3
Plasmid#221260PurposeExpression of IRF3 in mammalian cells by retroviral transductionDepositorInsertIRF3 (Irf3 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LPAR2-DuET
Plasmid#213330PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertLPAR2 (LPAR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRPM7 gRNA (BRDN0001147821)
Plasmid#76112Purpose3rd generation lentiviral gRNA plasmid targeting human TRPM7DepositorInsertTRPM7 (TRPM7 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMX-Baf155
Plasmid#25856DepositorInsertBrg1 associated factor 155 (Smarcc1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-ovoD1
Plasmid#111142PurposeExpresses U6:3-sgRNA targeting ovoD1 mutation in Drosophila melanogasterDepositorInsertpCFD3-ovoD1 (ovo Fly)
UseCRISPRTagsExpressionInsectMutationPromoterU6:3Available sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-rat Nrxn3beta AS4(-)
Plasmid#58269PurposeExpression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)DepositorInsertNrxn3beta AS4(-) (Nrxn3 Rat)
UseTagsHAExpressionMammalianMutationPromoterCAGAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-iLACCO1.2
Plasmid#218823PurposeMammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinityDepositorInsertiLACCO1.2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ACE2-GFP
Plasmid#154962PurposepcDNA3.1-ACE2-GFPDepositorInsertACE2-GFP (ACE2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TBG-ER-TurboID
Plasmid#149415PurposeHepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas ServiceAAV8InsertER-TurboID
UseAAVTagsHA and V5ExpressionMutationPromoterTBGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ovoD1 D1B2NR (18 kb)
Plasmid#37280DepositorInsertovoD1 genomic DNA fragment
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/BLM
Plasmid#111766PurposeTo express human BLM protein in human cellsDepositorInsertBLM (BLM Human)
UseTagsFlag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCaggs-mGold2s
Plasmid#231763PurposeExpression of mGold2t in mammalian cellsDepositorInsertmGold2s
UseTagsExpressionMammalianMutationmGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…PromoterActin promoter with CMV enhancerAvailable sinceMarch 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
2C::tdTomato Reporter
Plasmid#40281DepositorInsert2C Promoter (2-cell stage promoter)
UseTagstdTomatoExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
Plasmid#158653Purposemaking transgenics expressing a UAS driven mCherry and NTR 2.0DepositorInsert5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
UseTol2-based transgenic expression in zebrafish/oth…TagsExpressionMutationF70A/F108YPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6b:sgRNA#3
Plasmid#64247Purposeexpresses sgRNA under U6b promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-Delta23C11orf83
Plasmid#65849Purposebacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)DepositorInsertUQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
UseTagsGSTExpressionBacterialMutationdeletion of the 23 first aa (TM)PromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG
Plasmid#187179PurposeExpresses green norepinephrine indicator nLightG in neuronal cellsDepositorHas ServiceAAV9InsertnLightG
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_HA-BirA
Plasmid#113896PurposeMammalian (inducible) expression of HA-tagged cytosolic E. coli biotin ligaseDepositorInsertE. coli biotin ligase
UseLentiviralTagsHAExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-deLACCO1-NGR
Plasmid#208024PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1972
Plasmid#143987PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTBX2 (TBX2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
TurboID-mNeongreen-3xFLAG fusion pos3
Plasmid#172864PurposeC. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion proteinDepositorInsertTurboID
UseUnspecifiedTagsFLAG, mNeongreen, and mycExpressionMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTRW26K_1Ti1
Plasmid#177292Purposegenomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFPDepositorInsertPsacB-sacB
UseSynthetic Biology; Yeast expression, rrn genomic …TagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDWJ26
Plasmid#207603PurposeGFP-AIA expressionDepositorInsertGFP-AIA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLASTp-DsRed2
Plasmid#17706DepositorInsertGLASTp (SLC1A3 Human)
UseTagsDsRed2ExpressionMammalianMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSGAb-spe
Plasmid#122000PurposeA sgRNA expression plasmid for genome editing in Acinetobacter baumanniiDepositorInsertnone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRVL-1
Plasmid#104579PurposeContains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.DepositorInsertMouse CL kappa
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2 GFP-P2A-BFP
Plasmid#202195Purposeknock-in donor vector of GFP-P2A-BFP to C-terminus of endogenous human PLIN2DepositorInsertPLIN2 (PLIN2 Human)
UseCloning vectorTagsGFP-P2A-BFP knock-inExpressionMutationPromoterlacAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2260
Plasmid#141998PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX17 (SOX17 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_HUWE1_3xFLAG
Plasmid#187155Purposemammalian cell expression of HUWE1-3xFLAGDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV JMJD3
Plasmid#21212DepositorInsertJMJD3 (KDM6B Human)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pKL123
Plasmid#187889PurposeAAVS1 safe-harbor integration of dox-inducible NFIA-SOX9DepositorInsertNFIA-SOX9
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K6
Plasmid#22900DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK6 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-URA3
Plasmid#224075PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selectionDepositorInsertymScarletI3
UseTagsymScarletI3ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-CaMKII-Jaws-KGC-tdTomato-ER2
Plasmid#153534PurposeAAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.DepositorInsertJaws-KGC-tdTomato-ER2
UseAAVTagsER2, KGC, and tdTomatoExpressionMammalianMutationK200R W214FPromoterCaMKIIAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-Bcl2
Plasmid#46971Purposelentiviral expression of Bcl2DepositorInsertBcl2 (BCL2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgTelomere(F+E)
Plasmid#51024PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeresDepositorInsertOptimized sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pCAT.000
Plasmid#119559PurposeSelf replication Level T acceptor vector with LacZ compatible blue/white screening. Modified BioBrick vector, RSF1010-derived ori.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABEmax-NRCH
Plasmid#136923PurposeExpresses ABEmax-NRCH in mammalian cellsDepositorInsertABEmax-NRCH
UseCRISPRTagsExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-mCherry-53BP1
Plasmid#127658PurposeOver-express mCherry-53BP1 fragment to monitor 53BP1 foci.DepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsmCherryExpressionMutationNucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…PromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-145
Plasmid#118529PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-iGABASnFR2n-WPRE
Plasmid#218875PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAVTagsExpressionMammalianMutationS99A F104H R168PPromoterSynapsinAvailable sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only
