164,577 results

1. pHBS838 TDP43 RRM-GFP

   Plasmid

   #98249

   Purpose

   TDP43 RRM-GFP reporter construct

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

   Insert

   TARDBP (TARDBP Human)
   

   Use

   Tags

   Internal GFP to replace RRM domains

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT114

   Plasmid

   #138523

   Purpose

   phage cloning

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   phage genes
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHSS6

   Plasmid

   #37060

   Depositor

   Hank Seifert, Fred Heffron

   Article

   Seifert et al Proc Natl Acad Sci U S A. 1986 Feb;83(3)

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCG-Hc∆18-AA

   Plasmid

   #86559

   Purpose

   Plasmid encodes blinded measles virus H glycoprotein

   Depositor

   Jakob Reiser

   Article

   Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

   Insert

   Measles virus H glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y463A, R515A

   Promoter

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJKR-H-tetR

   Plasmid

   #62561

   Purpose

   Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-CMV-Galpha-i3 ONE-GO

   Plasmid

   #189737

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-i3 ONE-GO
   GNAI3 (Gnai3 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. TFORF2494

   Plasmid

   #142112

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB18 (ZBTB18 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-7 asyn A53T

   Plasmid

   #105727

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A53T (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A53T

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOxyR_gfp

   Plasmid

   #194154

   Purpose

   Green Fluorescent Reporter of intracellular hydrogen peroxide

   Depositor

   Sven Panke

   Article

   Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

   Insert

   transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PoxyR, PoxyS

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV2-DEST(f)

    Plasmid

    #190226

    Purpose

    Gateway destination vector, empty AAV vector backbone

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-AML1/ETO-IRES-GFP

    Plasmid

    #60832

    Purpose

    AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    AML1/ETO (RUNX1, RUNX1T1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDC-UAS-nBitbow1.0(miniW)

    Plasmid

    #138329

    Purpose

    Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.

    Depositor

    Dawen Cai

    Article

    Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3

    Inserts

    mAmetrine
    tdKatushka2
    mNeonGreen
    mTFP1
    mKusabira-Orange2
    

    Use

    Tags

    human H2B

    Expression

    Insect

    Mutation

    Promoter

    10xUAS

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE puro PPAR delta

    Plasmid

    #8891

    Depositor

    Bruce Spiegelman

    Article

    Brun et al Genes Dev. 1996 Apr 15. 10(8):974-84.

    Insert

    PPAR delta (Ppard Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N3/hArf6(WT)-EGFP

    Plasmid

    #79423

    Purpose

    Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLPO1

    Plasmid

    #209975

    Purpose

    Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (256_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF0641

    Plasmid

    #143232

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    THAP11 (THAP11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK- Ma PylRS WT

    Plasmid

    #197573

    Purpose

    Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Insert

    M. alvus PylRS WT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    GlnS (constitutive)

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. DDX5

    Plasmid

    #155523

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-3c-rhVTN_FL

    Plasmid

    #30223

    Depositor

    James Thomson

    Article

    Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

    Insert

    rhVitronectin without deletions (VTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No deletions

    Promoter

    Available Since

    May 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Flag-Gsdmd-CT

    Plasmid

    #80953

    Purpose

    Expresses Gsdmd-CT in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-CT (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLV CMV mCherry-DDX1-V5

    Plasmid

    #175162

    Purpose

    Lentiviral expression of human DDX1-V5

    Depositor

    Larry Gerace

    Article

    Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

    Insert

    DDX1 (DDX1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AAVS1-CAG-hrGFP

    Plasmid

    #52344

    Purpose

    AAVS1 donor vector for constitutive gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    hrGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHH21-BNA-Luc

    Plasmid

    #187924

    Purpose

    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymease I Promoter

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pME-mScarlet-I-P2A-iCre (JDW 917)

    Plasmid

    #224516

    Purpose

    A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mScarlet-I-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCryptDel4.8

    Plasmid

    #141293

    Purpose

    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Inserts

    RelB
    gRNA targetting pMUT2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Native promoter from pMUT2 relB/relE operon and P…

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZS2oxySp-GFP-proD-oxyR

    Plasmid

    #78224

    Purpose

    synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

    Insert

    synthetic gene circuit
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PB_TetON_mCherry_P2AT2A_GFP-nb-KS

    Plasmid

    #238240

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. T7-LacO-GFP_ColE1

    Plasmid

    #171648

    Purpose

    Expresses GFP in BL21(DE3) E. coli. Positive control for expression experiments. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-LacO-GFPssrA-T7 Stop
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TFORF1906

    Plasmid

    #141883

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPB-PGK-destination

    Plasmid

    #60436

    Purpose

    Destination vector with PGK - Hygromycin selection marker

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. c-myc promoter (Del 1)

    Plasmid

    #16601

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 1) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-1 (XmnI->PvuII)

    Promoter

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET-28a mScarlet

    Plasmid

    #178543

    Purpose

    The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

    Depositor

    Siegfried Musser

    Article

    Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

    Insert

    mScarlet
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHS2056

    Plasmid

    #239837

    Purpose

    OMEGAOff + omegaRNA scaffold AAV expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    DNMT3A-DNMT3L-NovaIscB-KRAB::NovaIscB omegaRNA scaffold
    

    Use

    AAV

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    Insertion of REC domain from NbaCas9-1 + E137K/E4…

    Promoter

    EFS; human U6

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pC1-REX-YFP

    Plasmid

    #48247

    Purpose

    Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    REX-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Flag-Keap1

    Plasmid

    #28023

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

    Plasmid

    #154774

    Purpose

    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-BG4-NLS-mVen

    Plasmid

    #211449

    Purpose

    mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

    Depositor

    Helmut Pospiech

    Insert

    Anti-DNA G-quadruplex single chain antibody BG4
    

    Use

    Tags

    NLS-mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge