173,197 results

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pX459V2.0-HypaCas9

Plasmid

#108294

Purpose

pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MCS-BirA(R118G)-HA

Plasmid

#36047

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA(R118G)-HA

Tags

HA

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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p5E-0.349cldn15la

Plasmid

#125026

Purpose

Five prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.

Depositor

John Rawls

Article

Murdoch et al PLoS Pathog. 2019 Mar 7;15(3):e1007381.

Insert

Claudin 15-like a (cldn15la Zebrafish)

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

Plasmid

#205540

Purpose

Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

TRE3GS

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-VSVG

Plasmid

#35616

Depositor

Arthur Nienhuis, Patrick Salmon

Insert

VSV G protein

Expression

Mammalian

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3'3xFlag

Plasmid

#208616

Purpose

Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

Depositor

Quanfu Ma

Type

Empty backbone

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mCherry-mTrim21

Plasmid

#105522

Purpose

T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mCherry

Promoter

T7

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-PKCZ

Plasmid

#110512

Purpose

Fluorescent PKCZ

Depositor

Bernardo Mainou

Insert

PKCZ (PRKCZ Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_Gag-EIF4E

Plasmid

#212657

Purpose

piggyBac vector to express an MLV Gag-EIF4E fusion protein

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Piggybac

Tags

EIF4E

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBF3060

Plasmid

#26853

Purpose

Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Insert

CreA recombinase

Use

Cre/Lox

Expression

Yeast

Mutation

N/A

Promoter

GAL1

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pDisplay-ZIBG2

Plasmid

#169458

Purpose

cell surface localized zinc sensor with ~ 300 nM Kd

Depositor

Huiwang Ai

Article

Chen et al Anal Chem. 2019 Oct 1;91(19):12212-12219

Insert

ZIBG2

Tags

pDisplay backbone elements for export and membran…

Expression

Mammalian

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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BPK2014-AaCas12b

Plasmid

#121949

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

10xHis tag

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-FLAG-CRBN-pGK-HYG

Plasmid

#107374

Purpose

Lentiviral expression of FLAG-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Tags

FLAG

Expression

Mammalian

Promoter

UbcP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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7xE-Box::Renilla

Plasmid

#124532

Purpose

c-Myc Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

c-Myc Renilla reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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Ctcf-Halo-mAID donor

Plasmid

#113103

Purpose

Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Mouse, Synthetic)

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

Plasmid

#207953

Purpose

nuclease inactive control for TRF1FokI WT

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI (D450A)

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-H2B-mCherry

Plasmid

#159655

Purpose

Empty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B mCherry nuclear reporter.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Type

Empty backbone

Use

CRISPR

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-dCas9-FLp300

Plasmid

#61356

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Human, Synthetic)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-cKit

Plasmid

#114293

Purpose

TET-inducible expression of c-Kit

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

cKit (Kit Mouse)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK3

Plasmid

#78815

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGR721 - SLD3p-TP-DNAP1.Badboy2

Plasmid

#222013

Purpose

Expresses the Badboy2 TP-DNAP1 variant under control of a weak promoter for reducing p1 copy number

Depositor

Chang Liu

Article

Rix et al Science. 2024 Nov 8;386(6722):eadm9073.

Insert

TP-DNAP1 BadBoy2

Expression

Yeast

Promoter

SLD3p

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
B2375_CRY2olig-mEGFP

Plasmid

#215109

Purpose

Expresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

CRY2olig-mEGFP (CRY2 Mustard Weed)

Tags

mEGFP

Expression

Mammalian

Mutation

E490G, 1-498

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-BirA-ER

Plasmid

#20856

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2008 May . 5(5):397-9.

Insert

BirA-ER

Expression

Mammalian

Mutation

C-terminal ER targeting sequence (KDEL)

Available Since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_BiCHATe27_Gq_dTomato

Plasmid

#213832

Purpose

AAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Insert

HA-GqDREADD-dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CXCR4

Plasmid

#98942

Purpose

Mammalian expression plasmid for untagged human CXCR4

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TEX264-GFP

Plasmid

#201925

Purpose

For lentiviral transduction of TEX264-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Lentiviral

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER20_mStrawberry

Plasmid

#161734

Purpose

Doxycycline-inducible expression of mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

mStrawberry

Use

Lentiviral; Destinatioin vector for gateway cloni…

Expression

Mammalian

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH2.105_PB-EPN24-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205550

Purpose

Express EPN24-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN4-EF2-ABCG2-IRES-Neo

Plasmid

#25983

Depositor

Ren-he Xu

Article

Zeng et al Stem Cells. 2009 Oct . 27(10):2435-45.

Insert

ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2 (ABCG2 Human)

Use

Lentiviral

Tags

EF1-apha promoter and IRES-Neo

Expression

Mammalian

Mutation

None

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCasTns(Ptr)-array 8

Plasmid

#175580

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)

Expression

Bacterial

Mutation

NO

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA c-Jun

Plasmid

#237333

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

c-Jun (JUN Human)

Tags

HA

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-DDX3X

Plasmid

#116730

Purpose

Lentiviral expression of DDX3X

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDX3X (DDX3X Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT_TO_eGFP_TIA1

Plasmid

#106094

Purpose

gene expression of EGFP-TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIA1 (TIA1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-O-FUW-KLF4-P2A-mCherry

Plasmid

#203855

Purpose

Tet-inducible lentiviral plasmid expressing KLF4-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

KLF4 (KLF4 Human)

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroSuper shAngptl4 hygro

Plasmid

#19154

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

small hairpin RNA against angiopoietin-like 4 (ANGPTL4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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173,197 results