Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

179,521 results

Showing: 8121 - 8160 of 179521 results

  3. pc DNA FRT TO- APPSwed/Ind

    Plasmid
    #114194
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Expression
    Mammalian
    Mutation
    Popular mouse model, overexpress 2 mutations: Swe…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114194-plasmid-map-sequence-id-224256

    Enlarge

  4. pC1-SypHer3s-IMS

    Plasmid
    #108120
    Purpose
    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.
    Depositor
    Vsevolod Belousov
    Publication
    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SypHer3s-IMS
    Tags
    ratiometricYFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108120-plasmid-map-sequence-id-262557

    Enlarge

  13. pBR322-TIMER

    Plasmid
    #103056
    Purpose
    medium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)
    Depositor
    Dirk Bumann
    Publication
    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1
    Insert
    TIMER-bac
    Expression
    Bacterial
    Mutation
    mutations: S4T and S179T
    Promoter
    constitutive ybaJp promoter
    Available since
    Nov. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103056-plasmid-map-sequence-id-199055

    Enlarge

  14. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid
    #101218
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    N692A, M694A, Q695A and H698A
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101218-plasmid-map-sequence-id-198035

    Enlarge

  15. pGL4-ERSE2-luc2P-Hygro

    Plasmid
    #101790
    Purpose
    Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)
    Depositor
    Seiichi Oyadomari
    Publication
    Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xERSE-II
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal TATA-box promoter with low basal activity
    Available since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101790-plasmid-map-sequence-id-194148

    Enlarge

  17. pL97

    Plasmid
    #78576
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter
    Depositor
    Xuming Mao
    Publication
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Expression
    Bacterial
    Promoter
    ermEp* promoter from S. erythraea
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    78576-plasmid-map-sequence-id-421926

    Enlarge

  20. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

    Enlarge

  24. pAAVS1-P-CAG-GFPluc2

    Plasmid
    #80493
    Purpose
    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP-luciferase fusion protein expression
    Depositor
    Knut Woltjen
    Publication
    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GFPluc2
    Use
    Donor vector (human)
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80493-plasmid-map-sequence-id-151598

    Enlarge

  27. pAAV-hDlx-Flex-GFP-Fishell_6

    Plasmid
    #83895
    Purpose
    Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
    Depositor
    Gordon Fishell
    Publication
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Has service
    AAV1, AAV2, AAV8, AAV9, and AAV Retrograde
    Insert
    eGFP
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hDlx
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83895-plasmid-map-sequence-id-160320

    Enlarge

  30. PR_GEF YFP (2XPDZ-YFP-LARG DH)

    Plasmid
    #80408
    Purpose
    Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells
    Depositor
    Michael Glotzer
    Publication
    Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)
    Tags
    YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80408-plasmid-map-sequence-id-151385

    Enlarge

  34. pCDNA3-STAT3-S727D

    Plasmid
    #73364
    Purpose
    Expression of STAT3-S727D
    Depositor
    Jie Chen
    Publication
    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    Tags
    FLAG tagged
    Expression
    Mammalian
    Mutation
    E16K S727D
    Promoter
    CMV
    Available since
    May 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73364-plasmid-map-sequence-id-423259

    Enlarge

  36. pEGFP-centrin-1

    Plasmid
    #72641
    Purpose
    The plasmid encodes centrin-1 with an N-terminal EGFP tag
    Depositor
    Michel Bornens
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    centrin-1 (CETN1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72641-plasmid-map-sequence-id-132790

    Enlarge

  39. pEF5B-FRT-control-APEX

    Plasmid
    #73187
    Purpose
    Expresses "control"-APEX (cytosolic) in mammalian cells
    Depositor
    Maxence Nachury
    Publication
    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    control-APEX (Nphp3 Glycine max, Mouse)
    Tags
    internal GFP tag
    Expression
    Mammalian
    Mutation
    changed glycine 2 to alanine
    Promoter
    EF1alpha
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73187-plasmid-map-sequence-id-139537

    Enlarge

Showing: 8121 - 8160 of 179521 results