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169,673 results

Showing: 10151 - 10200 of 169673 results

  1. GPER-DuET

    Plasmid
    #213256
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPER (GPER1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213256-plasmid-map-sequence-id-430806

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  2. pYPQ265E2

    Plasmid
    #164719
    Purpose
    Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    A3A(Y130F)-zCas9(D10A)-UGI
    Use
    CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…
    Expression
    Plant
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164719-plasmid-map-sequence-id-325392

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  3. pKD239-1g-2

    Plasmid
    #90959
    Purpose
    Expresses S. baltica TtrR and mCherry constitutively and sfGFP under the PttrB185-269 promoter
    Depositor
    Jeffrey Tabor
    Article
    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi
    Inserts
    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    Expression
    Bacterial
    Promoter
    Bba_J23100, Bba_J23109, and PttrB185-269
    Available Since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    90959-plasmid-map-sequence-id-178329

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  15. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Article
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available Since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  30. TSC2-fl

    Plasmid
    #24940
    Depositor
    Kunliang Guan
    Insert
    TSC2 (Tsc2 Mouse)
    Use
    N/a
    Available Since
    Sept. 10, 2010
    Availability
    Academic Institutions and Nonprofits only

  32. pMSCV-Mic60

    Plasmid
    #191980
    Purpose
    Expression of V5-tagged Mic60, a component of the MICOS complex.
    Depositor
    Luca Scorrano
    Insert
    Mic60-V5
    Use
    Retroviral
    Available Since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191980-plasmid-map-sequence-id-379609

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  33. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

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  36. pISce1-D2EGFP

    Plasmid
    #32674
    Depositor
    Michael Tsang
    Type
    Empty backbone
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Available Since
    Nov. 18, 2011
    Availability
    Academic Institutions and Nonprofits only
    32674-plasmid-map-sequence-id-38116

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  38. TTC5

    Plasmid
    #39064
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TTC5 (TTC5 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. pAAV.synP.DIO.EGFP.WPRE.hGH

    Plasmid
    #100043
    Purpose
    An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
    Depositor
    Ian Wickersham
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    synapsin-1
    Available Since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100043-plasmid-map-sequence-id-193613

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  42. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  46. pTF-lacZ-rfp

    Plasmid
    #153037
    Purpose
    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153037-plasmid-map-sequence-id-318855

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  49. pET-APOBEC1-YTH

    Plasmid
    #200158
    Purpose
    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification
    Depositor
    Kate Meyer
    Article
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Insert
    APOBEC1-YTH
    Tags
    6xHis, HA, and Maltose Binding Protein
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available Since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200158-plasmid-map-sequence-id-396323

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Showing: 10151 - 10200 of 169673 results