161,152 results

1. pCAG-FLAG-puro-PTPIP51(1-470)

   Plasmid

   #170536

   Purpose

   Expresses PTPIP51(1-470) in human HeLa cells after transfection

   Depositor

   Byung Il Lee

   Article

   Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

   Insert

   Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

2. TLCV2-RB1

   Plasmid

   #87836

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Insert

   sgRB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter and U6

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

   Plasmid

   #111585

   Purpose

   An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   myriRFP670V5-P2A-post-eGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-Ef1a-hMGT-PGK-puro

   Plasmid

   #172394

   Purpose

   Lentiviral expression vector for human direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

   Insert

   Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Ef1a-mCherry-IRES-Cre

   Plasmid

   #55632

   Purpose

   Expresses Cre in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Inserts

   mCherry
   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. iC13 PB-TO-oNgn2-BFP-puro

   Plasmid

   #204733

   Purpose

   Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Optimized neurogenin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GPI-RpHLuorin2

   Plasmid

   #171721

   Purpose

   GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 fused to GPI anchor
   

   Use

   Tags

   ratiometric pHluorin2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG mito-GCaMP5G

   Plasmid

   #105009

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   GCaMP5G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ezh2[FL]-dCas9

   Plasmid

   #100086

   Purpose

   Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   14056 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[FL]-dCas9-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSin Capsid

    Plasmid

    #127693

    Purpose

    Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    Sindbis Capsid
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

    Plasmid

    #216694

    Purpose

    Synchronize trafficking of GPI-AP from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    SBP-mApple-FIREtag-GPI
    

    Use

    Tags

    FIREtag, IL2 signal sequence, Streptavidin Bindin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-DFCP1

    Plasmid

    #86746

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human DFCP1 (ZFYVE1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-HDAC6.ΔBuz-FLAG

    Plasmid

    #30484

    Depositor

    Tso-Pang Yao

    Article

    Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of the C-terminal Ubiquitin binding doma…

    Promoter

    Available since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-BCR-ABL-p190-FLAG

    Plasmid

    #205620

    Purpose

    Expression of BCR-ABL oncogene in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

    Insert

    BCR-ABL oncogene, p190 isoform b3a2
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T117M- Please see depositor comments

    Promoter

    CMV

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPD543 ZF21x6-Compact_EYFP

    Plasmid

    #138857

    Purpose

    EYFP reporter vector for the ZF21x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF21x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF21x6-Compact

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPD528 VP16-ZF21

    Plasmid

    #138757

    Purpose

    Constitutive expression of VP16-ZF21 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF21
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mEmerald-Clathrin-15

    Plasmid

    #54040

    Purpose

    Localization: Clathrin Vesicles, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. FUGW-APPSwe-T2A-Hygro

    Plasmid

    #190805

    Purpose

    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance gene

    Depositor

    Ronald Hart

    Insert

    APP (APP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Swedish (KM670/671NL)

    Promoter

    Ubi C

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid

    #100834

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CamKII

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1_RFP

    Plasmid

    #206177

    Purpose

    mammalian expression of RFP

    Depositor

    Christopher Goodnow

    Article

    Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

    Insert

    RFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR223_IDH1_p.R132H

    Plasmid

    #81686

    Purpose

    Gateway Donor vector containing IDH1, part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, Jesse Boehm, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R132H

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-3'3xFlag

    Plasmid

    #208616

    Purpose

    Empty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlag

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-FGFR3-S249C

    Plasmid

    #116386

    Purpose

    Lentiviral expression of FGFR3 S249C

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3 (FGFR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S249C

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pC1-CMV-mito-DAAO

    Plasmid

    #141132

    Purpose

    Expresses DAAO with mitochondrial localization signal in mammalian cells

    Depositor

    Vsevolod Belousov

    Insert

    mito-DAAO
    

    Use

    Tags

    2 mitochondrial localization signals and 7xHis

    Expression

    Mammalian

    Mutation

    deleted DAAO amino acids 367-368

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-mOXT-hM3Dq-mCherry-WPRE

    Plasmid

    #70717

    Purpose

    Expresses DREADDs Gq receptor under oxytocin promoter control

    Depositor

    Daniel Geschwind

    Article

    Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    mouse oxytocin

    Available since

    Jan. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-C-STAT1-mGFP-P2A-Puro

    Plasmid

    #199345

    Purpose

    express murine STAT1 protein fused with mGFP

    Depositor

    Massimo Mallardo

    Article

    Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

    Insert

    signal transducer and activator of transcription 1 (Stat1 Mouse)
    

    Use

    Lentiviral

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. myc-GABAAR Gamma2

    Plasmid

    #127619

    Purpose

    N-terminal myc-tagged Gamma2 subunit of GABAAR

    Depositor

    Josef Kittler

    Article

    Arancibia-Carcamo et al Proc Natl Acad Sci U S A. 2009 Oct 13;10

    Insert

    GABA-A receptor subunit gamma-2
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TFORF1039

    Plasmid

    #143908

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TSC22D1 (TSC22D1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSFV-Helper1

    Plasmid

    #92073

    Purpose

    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Insert

    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    

    Use

    Helper plasmid, for run-off transcription and rna…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLX307-VHL-3F-BirA

    Plasmid

    #220147

    Purpose

    Expresses VHL-BirA for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF-1a

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pScaf

    Plasmid

    #111401

    Purpose

    Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDD131

    Plasmid

    #119886

    Purpose

    Optimized aTc inducible pTet promoter for Rhodococcus opacus expressing mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

    Insert

    pTet_mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pC034 - LwCas13a-msfGFP-2A-Blast

    Plasmid

    #91924

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA Flag-RARalpha

    Plasmid

    #35555

    Depositor

    Harish Srinivas, Jonathan Kurie

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. hSirt3-RFP

    Plasmid

    #216806

    Purpose

    Human Sirt3-RFP driven by the CaMKII promoter

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

    Insert

    Sirt3 (SIRT3 Human)
    

    Use

    Lentiviral

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pQE30-His-AlaRS

    Plasmid

    #124103

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Alanyl-tRNA ligase (alaS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TFORF3138

    Plasmid

    #144614

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPARG (PPARG Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AAV-ReaChR-citrine

    Plasmid

    #50954

    Purpose

    ReaChR-citrine in AAV2 vector under human synapsin promoter

    Depositor

    Roger Tsien

    Article

    Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

    Insert

    ReaChR-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    Promoter

    truncated human Synapsin promoter

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. dCas9 fused to nanobody (anti-beta catenin)

    Plasmid

    #186420

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-nanobody fusion (anti-beta catenin)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. YFP-ICP0FxE-C3

    Plasmid

    #134561

    Purpose

    Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)

    Depositor

    Susan Janicki

    Article

    Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

    Insert

    ICP0
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    RING finger deletion (FXE)

    Promoter

    CMV

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Lenti_gRNA-Puro

    Plasmid

    #84752

    Purpose

    Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

    Plasmid

    #55643

    Purpose

    vCre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV; Vcre-dependent chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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44. wt Opa1

    Plasmid

    #204427

    Purpose

    Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 protein.

    Depositor

    Keir Neuman

    Article

    Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

    Insert

    hOpa1 isoform 1 (OPA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. AAV2-hSyn-G-Flamp1

    Plasmid

    #188569

    Purpose

    green biosensor for cAMP in neurons

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    AAV

    Tags

    His tag

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. Nsp8-EGFP

    Plasmid

    #165113

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp8 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid

    #58477

    Purpose

    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Changed Arginine 62 to Aspartic Acid

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA5/FRT/TO GFP HSPH1

    Plasmid

    #19480

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    HSPH1 (HSPH1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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49. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104907

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLV_Stargazin-mTurquoise2-iLID

    Plasmid

    #161001

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    Stargazin-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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