169,673 results
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Plasmid#177765PurposeOverexpression of 3xFLAG-6xHis tagged human GRHL1DepositorInsertGRHL1 (GRHL1 Human)
ExpressionMammalianAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
GRK2-K220R
Plasmid#35403DepositorAvailable SinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-CoffFon-eGFP-W3SL
Plasmid#218750PurposeProvides NOT intersectional (Flp NOT Cre) expression of eGFPDepositorInserteGFP
UseAAVPromoterhSynAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-R399E
Plasmid#122691PurposeExpress His-tagged PKM2 (R399E) protein in bacteria.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus NB
Plasmid#103986PurposePlasmid encoding mVenus NBDepositorInsertmVenus NB
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP
Plasmid#176283PurposeRecombinant rabies virus expressing histone-EGFPDepositorAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK p2A mCherry
Plasmid#117689PurposeCMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencingDepositorInsertBARKrgs peptide
TagsHA / FLAGExpressionMammalianAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-eGFP-NLS-RNF168 delta RING
Plasmid#133983Purposemammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)DepositorInsertRNF168 (RNF168 Human)
TagseGFPExpressionMammalianMutationdeletion of RING; siRNA resistant sequence: GAGGA…PromoterCMVAvailable SinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
SPLICS PM-ER Short P2A
Plasmid#164112PurposeDetect the short Plasma membrane-Endoplasmic reticulum contactDepositorInsertSplit YFP PM-ER Short P2A
ExpressionMammalianAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
rAAV2/MG1.1
Plasmid#184540PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.1 capsidDepositorInsertAAV-MG1.1 capsid
UseAAV; Helper vector for packagingMutationchanges on AAV9 capsid: K449R, A587L, Q588M; inse…Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-EGFP
Plasmid#107721PurposeFor expression of sgRNA from the U6 promoter with EGFP expression simultaneouslyDepositorTypeEmpty backboneUseCRISPRAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-BCKDK
Plasmid#23794DepositorInsertBCKDK (BCKDK Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-tdTomato
Plasmid#44332DepositorHas ServiceAAV5InserttdTomato
UseAAV; Adeno-associated virus ()ExpressionMammalianAvailable SinceJuly 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti.pFOXA2.GFP
Plasmid#59404Purposeexpresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during developmentDepositorInsert400 bp FOXA2 promoter, eGFP (FOXA2 Human)
UseLentiviralExpressionMammalianPromoterpFOXA2Available SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
T7-AglC-His6
Plasmid#89726PurposeExpresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.DepositorInsertAglC
TagsHis6 and T7ExpressionBacterialAvailable SinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
AA029
Plasmid#216009PurposeFragmid fragment: (Cas protein) nickase Cas for base editingDepositorHas ServiceCloning Grade DNAInsertnCas9-NG (D10A)_v1.1 [Sp]
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-SPHK1
Plasmid#23704DepositorInsertSPHK1 (SPHK1 Human)
UseGateway donor vectorAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMVhPOP1_3Flag
Plasmid#53968PurposeMammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.DepositorAvailable SinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
2Flag-pDEST-C
Plasmid#118372PurposeGateway compatible mammalian expression vector with C-terminal 2Flag to tag proteins of interest.DepositorTypeEmpty backboneUseDestinationTags2FlagExpressionMammalianAvailable SinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-IS186
Plasmid#175581PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
ExpressionBacterialMutationNOAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-BirA-ER
Plasmid#20856DepositorInsertBirA-ER
ExpressionMammalianMutationC-terminal ER targeting sequence (KDEL)Available SinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
Tol2-elavl3-GCaMP6s
Plasmid#59531PurposeExpressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backboneDepositorInsertsAvailable SinceSept. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2 sgKEAP1-2
Plasmid#186459Purposeknock out KEAP1 in mammalian cellsDepositorInsertKeap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
UseCRISPR and LentiviralAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTG612
Plasmid#237387PurposemStayGold^NSEC^ repair templateDepositorInsertmStayGold^NSEC^
Available SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTG613
Plasmid#237388PurposemStayGold::AID^NSEC^ repair templateDepositorInsertmStayGold::AID^NSEC^
Available SinceJan. 28, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAL750 :: msfGFP
Plasmid#250661PurposeInducible promoter Pxyl expressing msfGFP in haloarcheon Haloferax volcaniiDepositorInsertMonomeric Superfolder Green Fluorescent Protein
UseHaloarchaeal expressionAvailable SinceJan. 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-FastFUCCI-P2A-H2A-tdiRFP (JDW 1402)
Plasmid#242591PurposeA CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an H2A-tdiRFP for nuclear labeling.DepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
ExpressionMammalianPromoterCAGGSAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTCas9_scr
Plasmid#207566PurposeThermoCas9-mediated cleavage of genomic DNA in E. coliDepositorInsertPlac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
ExpressionBacterialMutationWild-typeAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pICSL12013
Plasmid#245630PurposeLevel 0 Golden Gate part 6GAL4UAS promoter, GGAG - AATGDepositorInsert6GAL4UAS promoter
UseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-FastFUCCI (JDW 1494)
Plasmid#242592PurposeA CAGGS driven expression vector containing FastFUCCI to label cell cycle state.DepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
ExpressionMammalianPromoterCAGGSAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mScarlet3-S2 (JDW 1514)
Plasmid#242552PurposeGateway middle entry clone containing H2B-mScarlet3-S2; Nuclear red fluorescent reporterDepositorInsertmScarlet3-S2
UseGateway subcloningAvailable SinceDec. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap.(cyto).iATPSnFR2.A95K.tdTomato
Plasmid#244921PurposeiATPSnFR2.A95K variant with non-responsive tdTomatoDepositorInsertiATPSnFR2.A95K.tdTomato
UseAAVMutationA95KAvailable SinceNov. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-Pho8∆60-HphMX6
Plasmid#240973PurposeTo endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection markerDepositorInsertPHO8∆60 (PHO8 Budding Yeast)
ExpressionBacterialAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-hCA4-IV77
Plasmid#244906PurposeRep/Cap plasmid for producing variant AAV-hCA4-IV77DepositorInsertSynthetic construct producing AAV REP/CAP
UseAAVExpressionMammalianAvailable SinceNov. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(mem).iGlucoSnFR2
Plasmid#244088PurposePlasma membrane targeted expression of green glucose sensorDepositorInsertiGlucoSnFR2
UseAAVTagsIgG kappa leaderAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(cyto).iGlucoSnFR2
Plasmid#244111PurposeCytosolic expression of green glucose sensorDepositorInsertiGlucoSnFR2
UseAAVAvailable SinceSept. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
NanoBiT Assay Vector, HER1(1-668)-LgBiT
Plasmid#237104PurposeExpress HER1(1-668)-LgBiT Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNAInsertHER1 (EGFR Human)
TagsLgBiTExpressionMammalianMutation1-668PromoterCMVAvailable SinceSept. 16, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Rat SARE-Arc Min Promoter-Flag Arc-SV40 3'UTR
Plasmid#233057PurposeTo express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains a SV40 3' UTRDepositorAvailable SinceAug. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-USP7-sh1
Plasmid#235527PurposeshRNA against human USP7DepositorInsertUSP7 (USP7 Human)
UseLentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-USP7-sh5
Plasmid#235528PurposeshRNA against human USP7DepositorInsertUSP7 (USP7 Human)
UseLentiviralAvailable SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
RNF168 C-terminal sgRNA
Plasmid#207100PurposepX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.DepositorInsertGAGATGCACAAAGTAAGGCC
ExpressionMammalianPromoterCMV and U6Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2_Rluc-Y0x-Fluc
Plasmid#233614PurposeExpression of Rluc-Fluc reporter gene containing no YDepositorInsertRluc-Y0x-Fluc
UseLuciferaseAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
SeqCBE
Plasmid#232115PurposeExpresses AncBE4max, nSeqCas9 (D10A), and puromycin resistance genesDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP
Plasmid#232608PurposePan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTRDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS3-5xQUAS+Δpes-10P::AI::eTsChR
Plasmid#232612PurposeExpression of opsin 'eTsChR' on activation via 'QF+hGR' chimeric proteinDepositorInserteTsChR
ExpressionWormMutationN/AAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_FL Gcn4
Plasmid#231855PurposeBacterial expression of N-terminally 6His tagged FL Gcn4DepositorInsertGcn4
Tags6xHis, TEV cleavage siteExpressionBacterialPromoterT7Available SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Aquamarine-LC3B G120A-TdLanYFP
Plasmid#228561PurposeEncodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoterDepositorAvailable SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHelper-CRISPRi-sgRNA
Plasmid#221135PurposeHelper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with dCas9 handle
UseCRISPRExpressionBacterialPromoterJ23119(SpeI)Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-CRISPRi
Plasmid#221136PurposeCoxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.DepositorInsert3xF-dCas9
UseCRISPRTags3xFLAGExpressionBacterialPromotercbu1169 promoterAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only