8,969 results

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pAKgfp1

Plasmid

#14076

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

Green fluorescent protein

Expression

Bacterial

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-Neurod1

Plasmid

#30129

Depositor

Marius Wernig

Article

Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

Insert

NeuroD1 (Neurod1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pGEM-Sox2

Plasmid

#16353

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)

Expression

Bacterial

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pLJM1 Flag Raptor Rheb15

Plasmid

#26634

Depositor

David Sabatini

Article

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

raptor-Rheb15 (RPTOR Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk1a1(APF)

Plasmid

#19730

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Mouse, Human)

Tags

Flag

Expression

Mammalian

Mutation

replaced dual phosporylation motif Thr(1959)-Pro-…

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-Xist-PA

Plasmid

#26760

Depositor

Rudolf Jaenisch

Article

Wutz et al Mol Cell. 2000 Apr . 5(4):695-705.

Insert

Xist (Xist Mouse)

Expression

Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-AHPH-DM

Plasmid

#71368

Purpose

Location sensor for RhoA-GTP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

C terminus of Anillin (ANLN Human)

Tags

EGFP

Expression

Mammalian

Mutation

A740D and E758K

Promoter

CMV

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP NFAT5 (no EGFP)

Plasmid

#13627

Depositor

Anjana Rao

Article

Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

Insert

NFAT5 (NFAT5 Human)

Tags

6xMyc

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-Bcatfl

Plasmid

#24193

Depositor

David Rimm

Insert

beta-catenin (CTNNB1 Human)

Tags

GST

Expression

Bacterial

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only
LeGO-EBFP2

Plasmid

#85213

Purpose

Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

Depositor

Boris Fehse

Article

Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

Insert

EBFP2

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLVPT-GDNF-tTR-KRAB

Plasmid

#11646

Purpose

Tet-regulated (Tet-on) lentiviral vector for GDNF (mPGK promoter) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GDNF, tTR-KRAB, Tet-on (GDNF Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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GABA(A) receptor subunit a1SE

Plasmid

#49168

Purpose

pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

Insert

GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)

Tags

myc and pHGFP (pHIuorin)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV JMJD3

Plasmid

#21212

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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pnos-Cas9-nos

Plasmid

#62208

Purpose

Expresses Cas9 under control of nanos promoter and 3'UTR. For germ line restricted genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

nanos

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pHRm-GFP(1-10)-VP64-NLS

Plasmid

#70228

Purpose

Expresses GFP(1-10)-VP64-NLS in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP(1-10)-VP64-NLS

Use

Lentiviral

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-GBP

Plasmid

#67650

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pQE30-His-IF3

Plasmid

#124125

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Initiation factor 3 (infC Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR1-YFP

Plasmid

#13014

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 1 (TLR1 Human)

Tags

YFP

Expression

Mammalian

Available Since

Nov. 3, 2006

Availability

Academic Institutions and Nonprofits only

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AAV pmSyn1-EBFP-Cre

Plasmid

#51507

Purpose

Can be used to generate AAV virus that will express Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV5

Insert

EBFP-Cre

Use

AAV

Promoter

pmSyn1

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLionII

Plasmid

#1730

Purpose

This is a 3rd generation lentiviral transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system

Depositor

Garry Nolan

Type

Empty backbone

Use

Lentiviral

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-3xFLAG-HIS3MX6

Plasmid

#20753

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 E

Plasmid

#158080

Purpose

Expresses SARS-CoV-2 envelope protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 envelope (E )

Expression

Mammalian

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDCC6

Plasmid

#59985

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Inserts

Cas9
U6-2>sgRNA

Use

CRISPR

Tags

3xFLAG

Promoter

U6-96Ab and hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAC1802_pmax-RBFOX1N-dCasRx-C

Plasmid

#118635

Purpose

Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-dCasRx-C

Use

CRISPR

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT miR-1 (aka:pTRE-m1d)

Plasmid

#14896

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

miR-1

Expression

Mammalian

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HshnRNPM

Plasmid

#64924

Purpose

Expresses V5- and SBP-tagged hnRNPM in mammalian cells

Depositor

Elisa Izaurralde

Insert

hnRNPM (HNRNPM Human)

Tags

V5 and SBP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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8,969 results