164,127 results

1. pcDNA3_2FLAG-AKAP79 WT

   Plasmid

   #183450

   Purpose

   Expression of human wild-type AKAP79

   Depositor

   Matthew Gold

   Article

   Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

   Insert

   AKAP5 (AKAP5 Human)
   

   Use

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2.2-mU6gRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

   Plasmid

   #72667

   Purpose

   Lentiviral dual CRISPR gRNA expression vector (mouse U6 and human U6 promoters)

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   mU6gRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pnEA-vH_His-TEV-SEPT2_SEPT6

   Plasmid

   #174497

   Purpose

   bacterial co-expression of human SEPT2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGG-GFP-TurboID-3XNLS

   Plasmid

   #209400

   Purpose

   Transiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID-3XNLS
   

   Use

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available Since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECE-M2-GPHN

   Plasmid

   #31665

   Depositor

   Anne Brunet

   Article

   Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

   Insert

   Gephyrin (GPHN Human)
   

   Use

   Tags

   M2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMOD_D4910

   Plasmid

   #127220

   Purpose

   Module D with ZmUbi1::BBM

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   BBM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   codon optimized CDSs

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHRSin_hTCRβ

   Plasmid

   #187361

   Purpose

   Lentiviral expression of GPa3b17 hTCR beta

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   GPa3b17 hTCR beta
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHRSin_hTCRα

   Plasmid

   #187360

   Purpose

   Lentiviral expression of GPa3b17 hTCR alpha

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   GPa3b17 hTCR alpha
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 SARS-CoV-2 M

   Plasmid

   #158078

   Purpose

   Expresses SARS-CoV-2 membrane protein in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 matrix (M )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFSW GFP IRES GFP

    Plasmid

    #133815

    Purpose

    Encodes EGFP that can be used as a control for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ132B2.0

    Plasmid

    #99888

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti6/V5-p53_R280K

    Plasmid

    #22933

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    R280K mutant

    Promoter

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pOSIP-TH (TetR, HK022)

    Plasmid

    #45988

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1

    Plasmid

    #222994

    Purpose

    Mammalian expression of iGeoCas9(C2) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C2)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-AMOT p130

    Plasmid

    #32821

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

    Insert

    AMOT p130 (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    E5D

    Promoter

    CMV

    Available Since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB_TRE3G_scFV-sfGFP-Setd2CD

    Plasmid

    #235579

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Setd2 (Setd2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.2/DEST/hTfR-HA

    Plasmid

    #69610

    Purpose

    human transferrin receptor with an HA tag fused at the C-terminus

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    human transferrin receptor (TFRC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-Axin1

    Plasmid

    #109370

    Purpose

    Transient over-expression of human Axin1

    Depositor

    Mariann Bienz

    Article

    Fiedler et al Proc Natl Acad Sci U S A. 2011 Feb 1;108

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-SynA

    Plasmid

    #174860

    Purpose

    Expresses mouse syncytin A in mammalian cells

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    syncytin A (Syna Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

    Plasmid

    #209651

    Purpose

    Expression of iATPSnFR2, high affinity

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    iATPSnFR2.S29W.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S29W.A95K

    Promoter

    CAG

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 6 x DBE-luc

    Plasmid

    #153160

    Purpose

    Luciferase reporter plasmid for FOXTF

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCI-SEP-GluR1

    Plasmid

    #24000

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR1 (Gria1 Rat)
    

    Use

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR1A-TAZ S89A

    Plasmid

    #179911

    Purpose

    Entry vector of TAZ S89A for gateway coloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Insert

    WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
    

    Use

    Entry vector

    Tags

    3X Flag-tag

    Expression

    Mutation

    Serine 89 mutated to Alanine

    Promoter

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pInducer20-EGFP-TEADiv2

    Plasmid

    #228876

    Purpose

    Tet-inducible lentiviral vector for expression of optimized GFP-TEAD inhibitor (TEADiv2)

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

    Insert

    TEADiv2
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PB-tetO-NB

    Plasmid

    #197092

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for sensory neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-T2A-BRN3A (NEUROG2, POU4F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPS2040

    Plasmid

    #1372

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    PGK1-U1A-ASH1 3' UTR (PGK1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

29. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEF1α-Cre-PGK-Puro

    Plasmid

    #207742

    Purpose

    Co-expression of the Cre recombinase and a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. CTV

    Plasmid

    #15912

    Depositor

    Klaus Rajewsky

    Article

    Thai et al Science. 2007 Apr 27. 316(5824):604-8.

    Insert

    CAG-STOP-eGFP-ROSA26TV
    

    Use

    Cre/Lox; /stop

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HPV-31neo

    Plasmid

    #153283

    Purpose

    Neomycin selectable HPV31 genome

    Depositor

    Elliot Androphy

    Article

    Gilson et al J Virol. 2020 Jul 30;94(16). pii: JVI.00

    Insert

    HPV-31 genome with Neo cassette
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    HindIII and CsiI site mutated from original Neo c…

    Promoter

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-ABE7.10

    Plasmid

    #102919

    Purpose

    expresses ABE7.10 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.10
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBS_12x-601_MA1

    Plasmid

    #114358

    Purpose

    12x Widom 601 nucleosome sequence

    Depositor

    Scot Wolfe

    Article

    Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

    Insert

    12X601-MA1
    

    Use

    Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTRE2-Bla(HA–RILP-FLAG)

    Plasmid

    #102424

    Purpose

    Dual tagged RILP for expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-Responsive

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pegRNA2_tevopreQ1

    Plasmid

    #212039

    Purpose

    Bacterial expression of the pegRNA for genome editing in E. coli

    Depositor

    Quanjiang Ji

    Article

    Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

    Insert

    pegRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    J23119

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Promoter

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-mouse PKA-RIbeta-mEGFP

    Plasmid

    #45526

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RI beta tagged by mEGFP
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNA3.1-Nanog

    Plasmid

    #28221

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    Nanog (NANOG Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONR223-ACVR2B

    Plasmid

    #23401

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ACVR2B (ACVR2B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    A333G, 1MM

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only