159,258 results

1. pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

   Plasmid

   #161926

   Purpose

   Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

   Insert

   rTetR(SE-G72P)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mPA-GFP-H2B-6

   Plasmid

   #57137

   Purpose

   Localization: Nucleus/Histones, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pcDNA3.1-MAP3K5 (WT)

   Plasmid

   #47104

   Purpose

   Expresses wild type human MAP3K5 in mammalian cells

   Depositor

   Yardena Samuels

   Insert

   MAP3K5 (MAP3K5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLVX neo ACTN1-GFP

   Plasmid

   #138293

   Purpose

   Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP fusion protein

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   ACTN1 (ACTN1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CaMKII-pAce-Kv2.1PR

   Plasmid

   #195529

   Purpose

   Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   pAce-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Ace-mNeon 78K, 81D, 92N, 178F; SY linker

   Promoter

   CaMKII

   Available since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-Tol1.zf1

   Plasmid

   #61388

   Purpose

   mRNA synthesis of tol1 transposase codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   tol1.zf1
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   sp6

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lamin A (R435C)-mRFP

   Plasmid

   #124274

   Purpose

   Lamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.

   Depositor

   Eric Schirmer

   Insert

   LMNA (R435C) (LMNA Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (R435C)

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG_Xph15-SNAPf-CCR5TC

   Plasmid

   #135536

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to SNAPf, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Use

   Tags

   SNAPf

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAL-c5X-ELP[AV-60]

   Plasmid

   #67012

   Purpose

   Expresses malE-ELP[AV-60] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-ELP[AV-60]
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH10

    Plasmid

    #188783

    Purpose

    Carries a NAT marker and C. albicans RBT5 promoter in a pGEM-T backbone

    Depositor

    Aaron Mitchell

    Insert

    RBT5 promoter
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pLenti6.2-Nanoluc-ccdB

    Plasmid

    #87078

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pRDB_347

    Plasmid

    #231114

    Purpose

    CBE activity-based selection cloning vector

    Depositor

    John Doench, David Root

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mCherry-CD9-10

    Plasmid

    #55013

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

    Plasmid

    #173452

    Purpose

    Contains mCherry-sfGFP tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

    Insert

    mCherry-sfGFP-ADH1ter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBMN_HA-TBK1 (kinase dead - D135N)

    Plasmid

    #199206

    Purpose

    Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D135N

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Talin1 head EGFP fusion

    Plasmid

    #221408

    Purpose

    Mammalian expression of Talin1 head EGFP fusion for mammalian expression

    Depositor

    Timothy Springer

    Article

    Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

    Insert

    Talin1 head EGFP fusion (TLN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG1237 FIREmate-ECFP-Fis1

    Plasmid

    #205173

    Purpose

    Expresses FIREmate-ECFP-Fis1 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    FIREmate-ECFP-Fis1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSEVA3b67Rb

    Plasmid

    #58311

    Purpose

    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA1 Flag ATXN1[30Q]

    Plasmid

    #33236

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 29 amino acids (27 Q…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pBa-LSS-GFP-LDLR wt

    Plasmid

    #98184

    Purpose

    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

    Depositor

    Gary Banker, Marvin Bentley

    Article

    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

    Insert

    LDLR (LDLR Human)
    

    Use

    Tags

    GFP and LDLR signal sequence, N-term of GFP so GF…

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta Actin

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pwtCas9-bacteria

    Plasmid

    #44250

    Purpose

    aTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    wild-type Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO-1

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA5-(-2)AP-Ub

    Plasmid

    #232582

    Purpose

    Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEHAC1-HA-BirAonly

    Plasmid

    #232584

    Purpose

    Express HA-BirA

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    BirA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5-AP-Ub-dGG

    Plasmid

    #232581

    Purpose

    Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5-AP(-3)-Ub

    Plasmid

    #232583

    Purpose

    Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5-AP-Ub

    Plasmid

    #232577

    Purpose

    Express Ubiquitin tagged with an Acceptor Peptide (AP)

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AviTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEHAC1-CHIP-GSGS-BirA-HA

    Plasmid

    #232588

    Purpose

    Express BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP.

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    CHIP - BirA (CHIP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Mutant ornithine transcarbamylase aa 30-114 deleted (OTC-delta)

    Plasmid

    #71878

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC-delta (Otc Rat)
    

    Use

    Tags

    Expression

    Mutation

    Deleted amino acid 30-114 from full length OTC

    Promoter

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. EGFP-Rab4a-7

    Plasmid

    #56440

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. CMV-Fast-1 wild-type

    Plasmid

    #16521

    Depositor

    Bert Vogelstein

    Article

    Zhou et al Mol Cell. 1998 Jul . 2(1):121-7.

    Insert

    FAST-1 (FOXH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

32. CMV Marina-T2A-nls-mCherry

    Plasmid

    #74216

    Purpose

    genetically-encoded fluorescent voltage indicator

    Depositor

    Vincent Pieribone

    Article

    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

    Insert

    fluorescent protein voltage sensor
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ci-VSP contains R217Q mutation; super ecliptic pH…

    Promoter

    CMV

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSBFP2-C1

    Plasmid

    #22880

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

    Insert

    SBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AAVS1-tdTomato

    Plasmid

    #159275

    Purpose

    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

    Insert

    tdTomato
    

    Use

    AAV; S1 knockin donor vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEP163

    Plasmid

    #137881

    Purpose

    PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    mRFP-T2A-BlasticidinR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRRL_SRSF2_WT_mCherry

    Plasmid

    #84020

    Purpose

    expression of SRSF2 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    2TA-mCherry and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. cpLID-micro

    Plasmid

    #168995

    Purpose

    circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    mCh-sspB-T2A-cpLID-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-HA-H-Ras_wt

    Plasmid

    #39503

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS (HRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

    Plasmid

    #233036

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

    Depositor

    Jonathan Ploski

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 3xFLAG-Halo-Dyskerin

    Plasmid

    #207578

    Purpose

    CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    HaloTag DKC1
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge