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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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159,258 results

Showing: 9241 - 9280 of 159258 results

  2. pAAV.CMV.ffLuciferase.SV40

    Plasmid
    #105532
    Purpose
    AAV expression of firefly luciferase from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105532-plasmid-map-sequence-id-202366

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  4. V1-STING

    Plasmid
    #124263
    Purpose
    N-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Use
    Tags
    Venus1
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124263-plasmid-map-sequence-id-241474

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  9. CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)

    Plasmid
    #140892
    Purpose
    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.
    Depositor
    Yasushi Okamura
    Article
    Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1
    Insert
    CiDr-VSP L223F-mCherry (tpte, vsp Zebrafish, Ciona intestinalis)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed 223L to F in Dr-VSP (zebrafish TPTE)
    Promoter
    Available since
    April 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    140892-plasmid-map-sequence-id-292709

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  13. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

    Plasmid
    #121966
    Purpose
    Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.
    Depositor
    Clifford Brangwynne
    Article
    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.
    Insert
    scFv-GCN4
    Use
    Lentiviral
    Tags
    superfold GFP-GB1-NLS-iLID
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121966-plasmid-map-sequence-id-236121

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  15. Lck-mTurquoise2

    Plasmid
    #98822
    Purpose
    In vivo visualization of the plasma membrane (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lck
    Use
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    98822-plasmid-map-sequence-id-190902

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  24. pKPI-MtLAP1

    Plasmid
    #193521
    Purpose
    Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots
    Depositor
    Jeremy Murray
    Article
    Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np
    Inserts
    MtLAP1
    DsRed
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    ATUBI10 and Medtr8g059790(KPI)
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193521-plasmid-map-sequence-id-382387

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  27. TFORF1259

    Plasmid
    #142770
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TP53 (TP53 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    142770-plasmid-map-sequence-id-395839

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  29. Lamin A (R435C)-mRFP

    Plasmid
    #124274
    Purpose
    Lamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R435C) (LMNA Human)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R435C)
    Promoter
    Available since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124274-plasmid-map-sequence-id-241955

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  35. SpCas9-NG-SsAPOBEC3B

    Plasmid
    #198536
    Purpose
    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198536-plasmid-map-sequence-id-388885

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  38. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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Showing: 9241 - 9280 of 159258 results