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162,660 results

Showing: 11801 - 11850 of 162660 results

  1. pCAG dCas9-KRAB-2A-EGFP

    Plasmid
    #92396
    Purpose
    CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    dCas9_KRAB_2A_EGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92396-plasmid-map-sequence-id-178234

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  4. TriEx-4 DHX36

    Plasmid
    #68368
    Purpose
    Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.
    Depositor
    James Vaughn
    Article
    Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2
    Insert
    DHX36 (DHX36 Human)
    Use
    Tags
    Histidine
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV ie enhancer/promoter
    Available since
    Sept. 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. CoV2-N-R203M

    Plasmid
    #177952
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203M). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (R203M) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R203M
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177952-plasmid-map-sequence-id-348187

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  26. Plenti-ARE-HSVTK-luc

    Plasmid
    #161789
    Purpose
    Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers
    Depositor
    Hans-Guido Wendel
    Article
    Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi
    Inserts
    Luciferase-T2A-HSV-TK
    mCherry P2A blasticidin
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and Murine Gclc ARE
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    161789-plasmid-map-sequence-id-324302

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  27. pDRM209 LTP (Luciferase tdTomato Puro)

    Plasmid
    #174723
    Purpose
    Expresses the firefly luciferase gene, E2A, tdTomato, T2A, and the puromycin resistance gene.
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    tdTomato
    pac
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and T2A linker
    Expression
    Mammalian
    Mutation
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174723-plasmid-map-sequence-id-348966

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  28. TFORF2079

    Plasmid
    #141911
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FLI1 (FLI1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141911-plasmid-map-sequence-id-397213

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  32. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6, mU6
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  36. TFORF0694

    Plasmid
    #141664
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    HNF4A (HNF4A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141664-plasmid-map-sequence-id-397916

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  41. pGL4.82(hRluc/Puro)

    Plasmid
    #212933
    Purpose
    Renilla luciferase vector for investigating regions controlling transcription
    Depositor
    Pete Stecha
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    212933-plasmid-map-sequence-id-419194

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  43. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51087
    Purpose
    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa 1.3 kb
    Available since
    Feb. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    51087-plasmid-map-sequence-id-77369

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  46. pGEX-Bcatfl

    Plasmid
    #24193
    Depositor
    David Rimm
    Insert
    beta-catenin (CTNNB1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  49. pJMP12042

    Plasmid
    #222354
    Purpose
    Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222354-plasmid-map-sequence-id-439060

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Showing: 11801 - 11850 of 162660 results