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We narrowed to 28,969 results for: Tat

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Showing: 10841 - 10880 of 28969 results

  1. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid
    #122681
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 153 to Alanine
    Available Since
    May 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    122681-plasmid-map-sequence-id-244746

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  4. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

    Plasmid
    #68345
    Purpose
    A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii
    Inserts
    puromycin
    hCas9
    Use
    CRISPR; Flp/frt
    Tags
    linked to red flouresent protein gene mKateII and…
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68345-plasmid-map-sequence-id-438782

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  5. pCMV6-AN-DDK_UBM1*2* POLI

    Plasmid
    #131232
    Purpose
    Mammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domains
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    DNA polymerase iota (POLI Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains inactivating mutations in both ubiquitin…
    Promoter
    CMV enhancer + CMV promoter
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131232-plasmid-map-sequence-id-267278

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  7. SBP-ST*-GFP

    Plasmid
    #163668
    Purpose
    Expresses SBP tagged partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Tags
    GFP and Streptavidin Binding Protein (SBP)
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163668-plasmid-map-sequence-id-322801

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  8. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid
    #156419
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Hyeryun Choe, Michael Farzan
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156419-plasmid-map-sequence-id-308855

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  15. pGST-HsSyx-375-792

    Plasmid
    #179292
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + GST + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179292-plasmid-map-sequence-id-354918

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  19. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid
    #69117
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    LL (566-567aa) mutated to AA. Silent mutations i…
    Promoter
    CMV
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69117-plasmid-map-sequence-id-122092

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  20. pET-FLAG-SpCas9-HF1-plus

    Plasmid
    #126775
    Purpose
    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126775-plasmid-map-sequence-id-437774

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  31. Venus-Bik-4E-pEGFP-C1

    Plasmid
    #166744
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    Bik-4E (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166744-plasmid-map-sequence-id-328837

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  32. Cav2.1 EA2, R57A, R59A pMT2

    Plasmid
    #206122
    Purpose
    expression of rabbit Cav2.1 calcium channel with a CaV2.1-P1217fs mutation causing a truncated protein, (EA2). R57A R59A mutations prevent dominant-negative suppression of Cav2.1 currents
    Depositor
    Annette Dolphin
    Article
    Dahimene et al Neurobiol Dis. 2016 Sep;93:243-56. doi:
    Insert
    cacna1a (Cacna1a Rat)
    Expression
    Mammalian
    Mutation
    Cav2.1-P1217fs, R57A, R59A
    Promoter
    Ad MLP/TPL/SV40
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206122-plasmid-map-sequence-id-415911

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  33. pAAV.hSyn.FLEX.FAPdL5-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105982
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular dL5 FAP fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-FAPdL5-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    FAPdL5-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Promoter
    human synapsin 1
    Available Since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105982-plasmid-map-sequence-id-235274

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Showing: 10841 - 10880 of 28969 results