We narrowed to 23,940 results for: nes

1. pXPR_213

   Plasmid

   #133456

   Purpose

   H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   Control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pG3K-U6SC

   Plasmid

   #134754

   Purpose

   pGreen3 CRISPR/Cas9

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   CRISPR/Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   gRNA-U6, zCas9-35SC

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-p27Luc

   Plasmid

   #23047

   Depositor

   William Kaelin

   Article

   Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

   Insert

   p27 (CDKN1B Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBS NL4-3 envFS eGFP Δnef/U3

   Plasmid

   #101346

   Purpose

   Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   delta nef/LTR

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH6HTC_BTF3-Halo

   Plasmid

   #175324

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   BTF3 (BTF3 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

   Plasmid

   #202025

   Purpose

   Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

   Depositor

   Mikhail Shapiro

   Article

   Hurt et al bioRxiv 2024.03.30.587094

   Insert

   Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GvpB S9G-R31L-R85L

   Promoter

   Available since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMOD_A0902

   Plasmid

   #91026

   Purpose

   Module A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TREX2-P2A-AtCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtUbi10

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

   Plasmid

   #214459

   Purpose

   Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pR6Kg-Alpha1

    Plasmid

    #165862

    Purpose

    Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mCherry-FKBP-SAC1C389S

    Plasmid

    #108128

    Purpose

    Recruitable inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(C389S)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pR6Kg-Omega1

    Plasmid

    #165864

    Purpose

    Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RFP-KIF3A-HL

    Plasmid

    #51397

    Purpose

    expression of RFP-tagged amino acid 369-701 (headless) of KIF3A in mammalian cells

    Depositor

    Trina Schroer

    Article

    Brown et al Traffic. 2005 Dec;6(12):1114-24.

    Insert

    KIF3A (Kif3a Mouse)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pSicoR human DGCR8-1

    Plasmid

    #14769

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    DGCR8 shRNA (DGCR8 Human)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pH6HTC_mmHUR-Halo

    Plasmid

    #175311

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HUR (Elavl1 Mouse)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-M2-TLR2

    Plasmid

    #12291

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Use

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I and H202Y; signal peptide (aa1-23) replaced …

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pETCON-PTX-S1-220

    Plasmid

    #67747

    Purpose

    Pertussis Toxin Subunit 1 Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    PTxS1-220
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPR_eGFP_191

    Plasmid

    #111821

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 171-191
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLQ-KO-tgt50

    Plasmid

    #126578

    Purpose

    CRISPR-Cas9 based efficient genome editing in S. aureus

    Depositor

    Sheng Yang

    Article

    Liu et al Acta Biochim Biophys Sin (Shanghai). 201

    Insert

    Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMA-SpCas9-g10

    Plasmid

    #80793

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPRIME-dsRed-shvgat

    Plasmid

    #71385

    Purpose

    Expresses dsRed and shRNA against vgat under the control of the CMV promoter in eukaryotic cells

    Depositor

    William Wisden

    Article

    Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

    Insert

    CMV promoter-dsRed-mir30-shvgat
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer/promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRL K-ras 3'UTR

    Plasmid

    #14804

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 Apr 1. ():.

    Insert

    K-ras 3'UTR (Kras Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pSG424 Smad2 (Gal4-Smad2)

    Plasmid

    #14932

    Depositor

    Joan Massague

    Article

    Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

26. optGluCl alpha EYFP

    Plasmid

    #15104

    Depositor

    Henry Lester

    Article

    Slimko et al J Neurosci Methods. 2003 Mar 30. 124(1):

    Insert

    GluCl alpha (glc-1 Nematode)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAG-nDsRedFL-miR9/9*

    Plasmid

    #177805

    Purpose

    The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOBCol2.3-GFPemd

    Plasmid

    #110210

    Purpose

    Labels cells in the late osteoblast lineage.

    Depositor

    David Rowe

    Article

    Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

    Insert

    eGFPemerald
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    2.3 kb rat type 1 collagen + first intron

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pG3B-U6SC

    Plasmid

    #134753

    Purpose

    pGreen3 CRISPR/Cas9

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    CRISPR/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    gRNA-U6, zCas9-35SC

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TagRFP-T-Rabenosyn-5

    Plasmid

    #37537

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    Rabenosyn5 (RBSN Human)
    

    Use

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pH6HTC_hsHUR-Halo

    Plasmid

    #175303

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HUR (ELAVL1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mCerulean3-Tubulin-C-18

    Plasmid

    #55450

    Purpose

    Localization: Microtubules, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

33. HA-TRIM28deltaCC2

    Plasmid

    #124957

    Purpose

    Mammalian expression of Human TRIM28 with deleted CC domain

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deletion of CC domains

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBI-GFP-Bub1-S492Y

    Plasmid

    #16625

    Depositor

    Bert Vogelstein

    Article

    Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

    Insert

    BUB1 S492Y (BUB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S492Y

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

35. attB53-Pac-attB53

    Plasmid

    #183610

    Purpose

    RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    Pac
    

    Use

    Rmce

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pALPS tat-P2A-rev

    Plasmid

    #101331

    Purpose

    Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 tat and rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti_CMV:Flag-GpNLuc_SV40:PuroR

    Plasmid

    #183037

    Purpose

    Expresses the FLAG-tagged GpNLuc (Green LumiFluor) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMM178

    Plasmid

    #61270

    Purpose

    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO, native, native (respectively)

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMJ915v2+Nterm Spe1 +Cterm Age1 site

    Plasmid

    #88914

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRU323

    Plasmid

    #167690

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge