4,133 results
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Plasmid#86198PurposeMultisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance markerDepositorTypeEmpty backboneUseGateway compatible attr1-attr2 destination vectorTagsExpressionPlantMutationPromoterArabidopsis ubiquitin 10 (AtUbi10)Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only
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pCMVR8.74
Plasmid#22036Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)DepositorInsertgag pol tat rev
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/rh10
Plasmid#112866PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap-rh10
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-NDi-CRISPRi (Gen1)
Plasmid#73497PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry markerDepositorInsertsdCas9-KRAB-P2A-mCherry
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-Nanobody
Plasmid#70696PurposeExpresses GST-tagged anti-mCherry Nanobody in E. coli cells.DepositorInsertmCherry-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE4
Plasmid#87087PurposeExpresses ApoE4 in Mammalian cellsDepositorInsertApolipoprotein E4 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDZ2087
Plasmid#92414PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
UseTagsMBP (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromotertacAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
Plasmid#60903PurposeExpressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activationDepositorInsertCas9 dead
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_WT
Plasmid#111906PurposeExpress RNASEH1 in mammalian cell.DepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-ADAR1-p150
Plasmid#117927PurposeADAR1-p150 expression plasmid.DepositorInsertADAR1-p150 (ADAR Human)
UseTagsmyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C3-hYAP1
Plasmid#17843DepositorInsertYAP (YAP1 Human)
UseTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermouse U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
Plasmid#60904PurposeThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64DepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-mCherry
Plasmid#114472PurposemCherry ControlDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
phH1-gRNA
Plasmid#53186PurposeExpresses the S. pyogenes sgRNA from the H1 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman H1Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhuman 7SKAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3-myr-AKT-HA
Plasmid#31789DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 myc CIITA (P#808)
Plasmid#14650DepositorInsertCIITA (CIITA Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6
Plasmid#39296DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR4-YFP
Plasmid#13018DepositorInsertToll-Like Receptor 4 (TLR4 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)
Plasmid#101139PurposeLuciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCardinal
Plasmid#51311PurposeExpresses far-red fluorescent protein mCardinal in mammalian cellsDepositorInsertmCardinal
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PARP1-3xFlag-WT
Plasmid#111575PurposeExpresses full length PARP1 in mammalian cellsDepositorInsertPARP1 (PARP1 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
deltaNp63alpha-FLAG
Plasmid#26979DepositorInsertΔNP63alpha (TP63 Human)
UseTagsFLAGExpressionMammalianMutationdominant negative isoform of p63; aa 1–587PromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2alpha-pcDNA3
Plasmid#18950DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseTagsHA-tagExpressionMammalianMutationwt cDNAPromoterAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbC-dsRED
Plasmid#84034PurposeContains Golden Gate cassette to express up to four gRNAs and dsREDDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2) cloning backbone
Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only