161,186 results

1. pGL3 BRE Luciferase

   Plasmid

   #45126

   Depositor

   Peter ten Dijke, Martine Roussel

   Article

   Korchynskyi et al J Biol Chem. 2002 Feb 15;277(7):4883-91.

   Insert

   Id1-BRE
   

   Use

   Luciferase; Bmp/smad transcriptional reporter

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDH-puro-Bcl-XL

   Plasmid

   #46972

   Purpose

   lentiviral expression of Bcl-XL

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPP085

   Plasmid

   #158795

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti CMV RasV12 Neo (w108-1)

   Plasmid

   #22259

   Depositor

   Eric Campeau

   Insert

   HRAS G12V (HRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V mutant

   Promoter

   Available since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-EGFP-C4-Nrf2

   Plasmid

   #21549

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

   Insert

   nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

   Plasmid

   #187583

   Purpose

   Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   PML (PML Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. polyQ74-GFP

   Plasmid

   #231893

   Purpose

   Forms cytosolic HTT partial exon 1 Q74 aggregates

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q74 (HTT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CAG repeat expansion

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF0500

   Plasmid

   #143709

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PPARGC1A (PPARGC1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPSU250

   Plasmid

   #173837

   Purpose

   Expresses 250 kD Penn State ladder protein

   Depositor

   Song Tan

   Article

   Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

   Insert

   STRHSTPABPACIL1bQRSSTRHSTPABMBPpepN
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CROPSeq-BFP

    Plasmid

    #196718

    Purpose

    CROP-Seq library plasmid with Chen tracrRNA (PMID: 24360272) and TagBFP

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pATetO 6XHis-Phusion

    Plasmid

    #206210

    Purpose

    Inducible expression of DNA polymerase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

    Insert

    Phusion DNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-PGK-Venus-Akaluc (neo)

    Plasmid

    #124701

    Purpose

    Aka-Luciferase reporter

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Akaluciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-tetO-Eomes

    Plasmid

    #70271

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Eomes

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    tetO

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEvolvR-enCas9-PolI5M

    Plasmid

    #113078

    Purpose

    Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    David Schaffer, John Dueber

    Article

    Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

    Insert

    enCas9-PolI5M
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-myc-CUL3

    Plasmid

    #19893

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    3xmyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-YFP

    Plasmid

    #13033

    Purpose

    Mammalian expression vector for cloning your gene fused to YFP

    Depositor

    Doug Golenbock

    Has Service

    Cloning Grade DNA

    Insert

    Yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3

    Plasmid

    #196936

    Purpose

    TGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Oshimori et al Cell. 2015 Feb 26;160(5):963-976. doi: 1

    Insert

    mCherry
    

    Use

    Lentiviral and RNAi

    Tags

    P2A-CreERT2

    Expression

    Mammalian

    Mutation

    Promoter

    SBE

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGAL4-DBD-MCS

    Plasmid

    #145245

    Purpose

    Backbone vector expressing GAL4 DNA-binding domain followed by MCS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GLUT1-GFP

    Plasmid

    #89440

    Purpose

    GLUT1 construct

    Depositor

    Jeffrey Rathmell

    Article

    Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

    Insert

    GLUT1 (Slc2a1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-OVA

    Plasmid

    #64599

    Purpose

    Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MSGV1-1D3-28Z.1-3 mut

    Plasmid

    #107227

    Purpose

    Anti-mouse CD19 CAR with CD3 ITAMs mutated

    Depositor

    James Kochenderfer, Steven Rosenberg

    Article

    Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

    Insert

    1D3-28Z. 1-3 mut
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    None

    Promoter

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PIK3CA-WT

    Plasmid

    #16643

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Science. 2004 Apr 23. 304(5670):554.

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMPE-ePPEplus

    Plasmid

    #205242

    Purpose

    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. SBE-NLS-mCherry PGK-rtTA3

    Plasmid

    #195332

    Purpose

    Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Inserts

    NLS-mCherry
    rtTA3
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and miniCMV

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pf hPGK mc2 IRES puro

    Plasmid

    #206237

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TFORF2545

    Plasmid

    #144038

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CEBPB (CEBPB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pcDNA3/BLM

    Plasmid

    #111766

    Purpose

    To express human BLM protein in human cells

    Depositor

    Ian Hickson

    Article

    Gaymes et al Oncogene. 2002 Apr 11;21(16):2525-33. do

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    Flag epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-CaMKIIa-EGFP

    Plasmid

    #50469

    Purpose

    EGFP under the control of CaMKIIa promoter

    Depositor

    Bryan Roth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EGFP-Rubicon

    Plasmid

    #28022

    Depositor

    Qing Zhong

    Article

    Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

    Insert

    Rubicon (RUBCN Human)
    

    Use

    Tags

    3xFlag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral vector

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUCmini-iCAP-AAV1.X1

    Plasmid

    #209780

    Purpose

    non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV1-X1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUC57-CmYLCV

    Plasmid

    #205245

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. XRE-H2B-mScarlet-I

    Plasmid

    #182296

    Purpose

    Fluorescent reporter for AhR activity

    Depositor

    Severine Degrelle, Thierry Fournier

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    mScarlet-I
    

    Use

    Tags

    m-Scarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. ePPEplus

    Plasmid

    #205241

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. (+)-Limonene Synthase

    Plasmid

    #112230

    Purpose

    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

    Depositor

    Daniel Oprian

    Article

    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

    Insert

    (+)-Limonene Synthase
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-52

    Promoter

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-TRE3g-mNeon-WPRE-pA

    Plasmid

    #192064

    Purpose

    mNeonGreen expression with TRE3g promoter

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3g

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-tMCK-ER-TurboID

    Plasmid

    #149412

    Purpose

    Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    ER-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    tMCK

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pLEX-MCS-ASC-GFP

    Plasmid

    #73957

    Purpose

    Express human ASC with a C-terminal EGFP tag in mammalian cells

    Depositor

    Christian Stehlik

    Article

    de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

    Insert

    PYD and CARD domain containing, PYCARD (PYCARD Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GFP-BAZ1B

    Plasmid

    #65372

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ1B (BAZ1B Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA3.1_PA-Cre

    Plasmid

    #122960

    Purpose

    Photoactivatable Cre recombinase based on split Cre fragments and Magnet system (pMag and nMag)

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

    Insert

    pcDNA3.1_PA-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. MSCV JMJD3

    Plasmid

    #21212

    Depositor

    Paul Khavari

    Article

    Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNP568

    Plasmid

    #222725

    Purpose

    pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100852

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. pLenti6.2_mNeonGreen2

    Plasmid

    #113727

    Purpose

    Expresses the fluorescent protein mNeonGreen2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Article

    Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

    Insert

    mNeonGreen2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pRPF215

    Plasmid

    #106377

    Purpose

    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

    Insert

    Ptet-himar1-Ter(slpA)-transposon
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Codon optimised for C. difficile

    Promoter

    Ptet

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pN7HELIX

    Plasmid

    #190662

    Purpose

    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    CMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge