20,110 results
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Plasmid#73148PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.DepositorInsertsHRPb-NLG (Nlgn1 Synthetic, Mouse)
UseLentiviralTagsV5 tagExpressionMammalianPromoterSynapsinAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171594PurposeStrong human ACE2 abundance from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…ExpressionMammalianAvailable SinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTNS3
Plasmid#63127PurposeTn7 transposase expressionDepositorTypeEmpty backboneExpressionBacterialPromoterP1 and PtacAvailable SinceMay 17, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA GFP10-ZIP
Plasmid#182241PurposeTripartite split-GFP mammalian. Express leucine zipper with GFP10DepositorInsertGFP10-ZIP
Tagssplit-GFP10 tripartiteExpressionMammalianPromoterCMVAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM3D(Gq)-mCherry
Plasmid#50460PurposeDouble floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-CRBN-pGK-HYG
Plasmid#107380PurposeMammalian expression of FLAG-CRBNDepositorAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA ZIP-GFP11
Plasmid#182242PurposeTripartite split-GFP mammalian. Express leucine zipper with GFP11DepositorInsertZIP-GFP11
Tagssplit-GFP11 tripartiteExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-Tight-MCS_PGK-GpNLuc
Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianPromoterPGKAvailable SinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-WTAP
Plasmid#53741PurposeFor expression of WTAP (Wilms tumor 1 associated protein) in human cellsDepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti puro HA-Ubiquitin
Plasmid#74218PurposeLentiviral expression of HA tagged UbiquitinDepositorInsertubiquitin (UBB Human)
UseLentiviralTagsHAExpressionMammalianMutationSingle ubiquitin coding sequence (full length UBB…PromoterCMVAvailable SinceMarch 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter
Plasmid#61762PurposeDual Color betaGlobin reporter to study splicing kinetics in vivoDepositorInsertb-Globin
Tags24 X MS2 hairpin cassette in 3'UTR of b-Glob…ExpressionMammalianPromoterDox inducible Tet-ON promoterAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
VRQR-ABEmax
Plasmid#119811PurposeA-to-G base editorDepositorInsertecTadA-ecTadA*(7.10)-nSpCas9 (VRQR)
ExpressionMammalianAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_D210N
Plasmid#111904PurposeExpress D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1DepositorAvailable SinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-BbsI-PITCh
Plasmid#127875PurposeFor user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assemblyDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM444
Plasmid#108319PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage IntegraseDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
ExpressionBacterialAvailable SinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
VCP(A232E)-EGFP
Plasmid#23973DepositorInsertValosin containing protein (VCP Human)
Tags6xHis, EGFP, and HAExpressionMammalianMutationA232EAvailable SinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
GPRC5B-Tango
Plasmid#66383PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPLICS PM-ER Long P2A
Plasmid#164111PurposeDetect the long Plasma membrane-Endoplasmic reticulum contactDepositorInsertSplit YFP PM-ER Long P2A
ExpressionMammalianAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PARP1-3xFlag-WT
Plasmid#111575PurposeExpresses full length PARP1 in mammalian cellsDepositorAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
TOM20*-SBP-GFP
Plasmid#120173PurposeExpresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)DepositorInsertMitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
TagsSBP and eGFPExpressionMammalianMutationTruncated TOM20: 1-30 aaPromoterCMVAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-FUCCI
Plasmid#118616PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianPromoterhPGKAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-BFP
Plasmid#78545PurposeFusion protein Cas9+BFPDepositorInsertBFP
UseLentiviralExpressionMammalianPromoterFusion protein with Cas9Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-(empty)-TAG
Plasmid#138209PurposeTo facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' regionDepositorTypeEmpty backboneUseT7 transcriptionTagsTAG sequenceExpressionMammalianPromoterCMV Promoter, T7 PromoterAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP_PrA/G-H6
Plasmid#176077PurposeExpress His6 tagged MBP_PrA/G in Escherichia coliDepositorInsertMBP PrA/G-H6
TagsHis tagExpressionBacterialPromoterT7 PromoterAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRepCap6
Plasmid#110770PurposepRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.DepositorInsertAAV6 cap, AAV6 rep genes
UseAAVAvailable SinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRLsinPGK_IRFP720_WPRE
Plasmid#91785PurposeConstitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.DepositorInsertiRFP720
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 KRAB-T2A-GFP
Plasmid#67620PurposeCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFPDepositorInserthumanized dead Cas9 KRAB T2A GFP
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
synIRES_RC25
Plasmid#189970PurposePart 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAsDepositorInsertsynIRES_RC25
UseCloningPromoterN/AAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NG-ABEmax
Plasmid#124163PurposeA-to-G base editorDepositorInsertTadA-TadA(evo)-Cas9-NG
ExpressionMammalianAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only