161,570 results

1. TFORF3007

   Plasmid

   #144483

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SMAD1 (SMAD1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-EKAR4

   Plasmid

   #174437

   Purpose

   Improved cyan/yellow FRET-based ERK kinase activity reporter.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   EKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-flex-ReaChR-citrine

   Plasmid

   #50955

   Purpose

   flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter

   Depositor

   Roger Tsien

   Article

   Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

   Insert

   ReaChR-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2998

   Plasmid

   #144474

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GATA2 (GATA2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-HA-HA-human CMYC S62A

   Plasmid

   #203436

   Purpose

   Mammalian expression of MYC

   Depositor

   Yoshiaki Ito

   Article

   Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

   Insert

   MYC (MYC Human)
   

   Use

   Tags

   2 x HA

   Expression

   Mammalian

   Mutation

   S62A

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. DH-BB(5'SIN;TE12ORF)

   Plasmid

   #72309

   Purpose

   Defective Helper to produce neurotropic, propagation-incompetent Sindbis virus

   Depositor

   Anthony Zador

   Insert

   DH-BB(5'SIN;TE12ORF)
   

   Use

   Sindbis virus helper construct

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEmerald-GPAT4152-208

   Plasmid

   #134468

   Purpose

   GPAT4152-208 expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   GPAT4 (GPAT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GPAT4, amino acids 152-208

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiPGK Puro DEST PKAKTRClover

   Plasmid

   #59153

   Purpose

   Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   PKA (PRKACA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2509

   Plasmid

   #144387

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   EP300 (EP300 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. Px461-Cas9n-Trp53-sgRNA-beta

    Plasmid

    #88847

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

11. Px461-Cas9n-Trp53-sgRNA-alpha

    Plasmid

    #88846

    Purpose

    CRISPR KO of Trp53

    Depositor

    Joan Massague

    Article

    Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

12. TFORF0924

    Plasmid

    #141757

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR2F2 (NR2F2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHM840

    Plasmid

    #20701

    Depositor

    Thomas Stamminger

    Article

    Sorg et al Biotechniques. 1999 May . 26(5):858-62.

    Insert

    GFP-NLS-lacZ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBA409

    Plasmid

    #85971

    Purpose

    EF1a-sfGFP-Ubc-Neo

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sfGFP
    Neo
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPM2213

    Plasmid

    #167553

    Purpose

    Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

    Depositor

    Renato Morona

    Article

    Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

    Insert

    rfb region of Shigella flexneri serotype 2a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-EGFP-C-IFT88

    Plasmid

    #218733

    Purpose

    Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

    Insert

    IFT88 (IFT88 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CSTB_pCSdest

    Plasmid

    #53910

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    CSTB (CSTB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    Sept. 8, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pEGFP NFAT5 (no EGFP)

    Plasmid

    #13627

    Depositor

    Anjana Rao

    Article

    Lopez-Rodriguez et al Proc Natl Acad Sci U S A. 1999 Jun 22. 9

    Insert

    NFAT5 (NFAT5 Human)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_GR-RE_MLPmin_BC1092-luc2

    Plasmid

    #227112

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1092

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1092 and a luciferase reporter gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMX-Brg1

    Plasmid

    #25855

    Depositor

    Hans Schöler

    Article

    Singhal et al Cell. 2010 Jun 11. 141(6):943-55.

    Insert

    Brahma related gene 1 (Smarca4 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pAAV-VTKS2-TVA-eGFP-N2cG

    Plasmid

    #175439

    Purpose

    Cre-on (DIO) helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

    Depositor

    Gordon Fishell

    Article

    Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

    Insert

    TVA-P2A-eGFP-P2A-N2cG
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV CAMKII PSAM4 GlyR IRES EGFP

    Plasmid

    #119744

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CamKII

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV-EB3mCherry_cmv-LifeActGFP

    Plasmid

    #133927

    Purpose

    Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

    Plasmid

    #113163

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCACTUS

    Plasmid

    #167559

    Purpose

    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do

    Type

    Empty backbone

    Use

    Suicide cloning vector, temperature sensitive.

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDual-eGFP

    Plasmid

    #63215

    Purpose

    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pLOX-CW-CRE

    Plasmid

    #12238

    Depositor

    Didier Trono

    Article

    Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

    Insert

    CRE
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LCV2 control

    Plasmid

    #217443

    Purpose

    Lentiviral vector expressing Cas9 without a targeting sgRNA

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHLUM

    Plasmid

    #40276

    Depositor

    Markus Ralser

    Article

    Mulleder et al Nat Biotechnol. 2012 Dec 7;30(12):1176-8

    Inserts

    LEU2 (LEU2 Budding Yeast)
    URA3 (URA3 Budding Yeast)
    MET15/17 (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous LEU2, endogenous MET15/17, and endogen…

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TFORF2541

    Plasmid

    #142399

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX5 (SOX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge