163,606 results

1. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

   Plasmid

   #228732

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

   Plasmid

   #228735

   Purpose

   Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEJS2697-CMV-Nme2SmuCas9-E932R

   Plasmid

   #228718

   Purpose

   Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2SmuCas9-E932R nuclease
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

   Plasmid

   #228731

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEJS2713-CMV-iNme2-ABE8e-i1-L10

   Plasmid

   #228734

   Purpose

   Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2 nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEJS2698-CMV-Nme2Cas9-D56R

   Plasmid

   #228719

   Purpose

   Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2Cas9-D56R nuclease
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 4_T7-mScarlet3-RitC

   Plasmid

   #225931

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-RitC
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. MLNR-DuET

   Plasmid

   #213339

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MLNR (MLNR Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. A*02:11:01

   Plasmid

   #203280

   Purpose

   Overexpress HLA allele

   Depositor

   Catherine Wu, Derin Keskin

   Article

   Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

   Insert

   A*02:11:01 (HLA-A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0807

    Plasmid

    #141712

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMARCC2 (SMARCC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-V5-SBP-C1-HsGIGYF1_AI

    Plasmid

    #148961

    Purpose

    Mammalian Expression of HsGIGYF1

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

    Insert

    HsGIGYF1 (GIGYF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pE-N1-RDH11

    Plasmid

    #161916

    Purpose

    Expresses untagged human RDH11. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Retinol dehydrogenase 11 (RDH11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ233-STU

    Plasmid

    #138111

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    dLbCas12a-SRDX
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AB.pCCL.sin.cPPT.GFP.miR-218-2-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85881

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-218-2-3p Sensor (MIR218-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. H2B-mCherry

    Plasmid

    #20972

    Purpose

    H2B fused to mCherry for chromatin visualization

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    Histone cluster 1, H2bb (H2BC3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUGW

    Plasmid

    #14883

    Purpose

    3rd gen lentiviral plasmid with hUbC-driven EGFP; can be used for cDNA expresion

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-GFP-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCW57.1

    Plasmid

    #41393

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKD4

    Plasmid

    #45605

    Depositor

    Barry L. Wanner

    Article

    Datsenko et al Proc Natl Acad Sci U S A. 2000 Jun 6;97(

    Insert

    FRT-kan-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    R6K

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMG36E

    Plasmid

    #133853

    Purpose

    Expression vector

    Depositor

    Jan Kok, Gerard Venema

    Article

    van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P32

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mTagBFP2-pBAD

    Plasmid

    #54572

    Purpose

    Localization: Protein Expression Vector , Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    6xHis, T7, and mTagBFP2

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-HA-Ubiquitin-WT

    Plasmid

    #17608

    Purpose

    Mammalian expression of HA tagged ubiquitin

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHCMV-AmphoEnv

    Plasmid

    #15799

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    4070A Amphotropic Murine leukemia virus envelope gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 3XFlag-pA-Tn5-Fl

    Plasmid

    #124601

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Steven Henikoff

    Article

    Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

    Insert

    Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
    

    Use

    Tags

    Protein A and Tn5 transposase fusion protein with…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMD2.G-K47Q/R354Q

    Plasmid

    #207323

    Purpose

    VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q/R354Q

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti CMV GFP Puro (658-5)

    Plasmid

    #17448

    Purpose

    3rd gen lentiviral eGFP expression vector, CMV promoter, Puro

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lck-mScarlet-I

    Plasmid

    #98821

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-N1-TFEB

    Plasmid

    #38119

    Purpose

    Mammalian expression of Transcription factor EB fused to EGFP

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFEB (TFEB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

    Plasmid

    #226475

    Purpose

    Improved microglia-targeted transgene expression

    Depositor

    Hirokazu Hirai

    Article

    Aoki et al Cell Rep Methods. 2025 Jul 25:101116. do

    Inserts

    mIba1 promoter, 1.7-kb, microglia-specific promoter
    target sequences for miR-9x4 & miR-129-2-3px4 *2
    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge