4,133 results
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pCAG-VSVL
Plasmid#64085PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain polymerase geneDepositorInsertVSV polymerase
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP1
Plasmid#22596DepositorInsertUSP1 (USP1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 GFP
Plasmid#10676PurposeRetroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
Plasmid#64216PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFPExpressionMammalianMutationPromoterCBh and U6Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMV306hsp
Plasmid#26155DepositorInserthsp60 promoter
UseMycobacteria integrating vectorTagsExpressionMutationPromoterAvailable sinceNov. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rubicon
Plasmid#28022DepositorInsertRubicon (RUBCN Human)
UseTags3xFlag and EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
LRG (Lenti_sgRNA_EFS_GFP)
Plasmid#65656PurposeLentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter-driven sgRNA expression and EFS promo…Available sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-NFAT1(4-460)-GFP
Plasmid#11107PurposeExpresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
UseTags3x HA and GFPExpressionMammalianMutationContains amino acids 4-460 of NFAT1; L78P compare…PromoterCMVAvailable sinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 MCS-BirA(R118G)-HA
Plasmid#36047PurposeTo fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tagDepositorHas ServiceCloning Grade DNAInsertBirA(R118G)-HA
UseTagsHAExpressionMammalianMutationR118G (highly promiscuous form)PromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro CDK4 R24C
Plasmid#11254DepositorInsertCDK4 (CDK4 Human)
UseRetroviralTagsExpressionMammalianMutationR24CPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
ppyCAG_RNaseH1_WKKD
Plasmid#111905PurposeExpress WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybridsDepositorInsertRNASEH1 (RNASEH1 Human)
UseTagsV5ExpressionMammalianMutationChanged D 210 to N, W 43 to A, K 59 to A, K 60 to…PromoterAvailable sinceJuly 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorInsertABE7.10
UseTagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-c-Myc
Plasmid#102626PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC proto-oncogene, bHLH transcription factor (MYC Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-LC3
Plasmid#21073PurposeMammalian expression of rat LC3 fused to EGFPDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseTagsEGFPExpressionMammalianMutationFirst ATG is not included.PromoterCMVAvailable sinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-U6:gRNA
Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ZO1-C-14
Plasmid#55165PurposeLocalization: Tight Junctions, Excitation: 587, Emission: 610DepositorInsertZO1 (TJP1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSCMV:tdTomato
Plasmid#30530Purposemammalian expression of tdTomato controlled by the ubiquitously active CMV promoter.DepositorHas ServiceCloning Grade DNAInserttdTomato
UseTagsExpressionMammalianMutationR415HPromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
T444T
Plasmid#113081PurposeEnhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.DepositorTypeEmpty backboneUseRNAiTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-puro
Plasmid#17923PurposeInsect expression vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseDrosophila metallothionein gene promoter drives e…TagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
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mouse E-cadherin GFP (423)
Plasmid#67937PurposeMammalian expression of E-cadherin-GFPDepositorInsertE-cadherin (Cdh1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Parkin
Plasmid#17613DepositorInsertParkin (PRKN Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42(Q61L)
Plasmid#12600DepositorInsertEGFP-Cdc42 (Q61L) (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationQ61LPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lyn-tailed mCherry-SEpHluorin
Plasmid#32002DepositorInsertMembrane-targeting sequence of Lyn kinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIRES-EGFP-puro
Plasmid#45567DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsIRES-EGFP-PuroExpressionMammalianMutationPromoterPhcmv (strong human cytomegalovirus immediate ear…Available sinceJune 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 SHP2 WT
Plasmid#8322DepositorInsertSHP2 (PTPN11 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_SOX2
Plasmid#26817DepositorInsert5'UTR-SOX2-3'UTR (SOX2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
MSCVhygro-F-G9a
Plasmid#41721PurposeRetroviral expression of human G9a in mammalian cellsDepositorInsertG9a (EHMT2 Human)
UseRetroviralTagsflagExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
moxBFP
Plasmid#68064PurposeC-terminal fusion to oxBFPDepositorTypeEmpty backboneUseTagsmoxBFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro largeTcDNA
Plasmid#14088DepositorInsertLarge T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEZYflag
Plasmid#18700PurposepFlag-CMV-2 converted Gateway Destination vector for expressing Flag tagged fusion proteins in mammalian cellsDepositorTypeEmpty backboneUseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
Beta-arrestin2 GFP WT
Plasmid#35411DepositorInsertβarrestin2 (Arrb2 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-LaminA-C-18
Plasmid#55068PurposeLocalization: Nuclear Envelope, Excitation: 587, Emission: 610DepositorInsertLamin A (LMNA Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CFP
Plasmid#11179PurposeMammalian expression vector for expression of CFPDepositorInsertECFP from Aequorea victoria
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT-HA-hM3D(Gq)
Plasmid#45547PurposeExpresses hM3D (Gq) neuronal activatorDepositorInserthM3D (Gq)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight Puro DEST (w768-1)
Plasmid#26430DepositorTypeEmpty backboneUseLentiviral; Tet-on advanced destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2010AvailabilityAcademic Institutions and Nonprofits only
