We narrowed to 10,805 results for: CHL

1. pKSB-AeU6-3gRNA

   Plasmid

   #183914

   Purpose

   gRNA-expressing plasmid under Aedres aegypti AAEL017763 U6 promoter

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   gRNA scaffold for protospacer cloning into BbsI sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Aedes aegypti U6 promoter (AAEL017763)

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA

   Plasmid

   #176854

   Purpose

   Expresses membrane bound biotin ligase, BioID2, in neurons

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Lck-13xLinker-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   hSyn1

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWPXL-Sox4

   Plasmid

   #36984

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox4 (SOX4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PZac2.1 hSyn1-HA-BioID2-SAPAP3

   Plasmid

   #176857

   Purpose

   Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   HA-BioID2-SAPAP3
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   hSyn1

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p207-Switch-ON (FRT)

   Plasmid

   #217886

   Purpose

   Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMDC123SB-AtMIR390a-B/c

   Plasmid

   #51775

   Purpose

   Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtMIR390a-B/c
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   A. thaliana MIR390a precursor sequence including …

   Promoter

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-Syn_CapChR2_mScarlet

   Plasmid

   #188039

   Purpose

   Expresses CapChR2-mScarlet in mammalian neurons

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   hSyn

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. U6-sgRNA-GFP

   Plasmid

   #200068

   Purpose

   For the knock down of GFP with an astrocyte specific mCherry expression

   Depositor

   Baljit Khakh

   Article

   Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

   Insert

   U6-sgRNA-GFP
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   GfaABC1D-mCherry

   Expression

   Mutation

   Promoter

   U6, GfaABC1D

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS L30 Ruby3-Rab6A

   Plasmid

   #135653

   Purpose

   Rab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

   Insert

   Rab6A
   

   Use

   Tags

   Ruby3

   Expression

   Mammalian

   Mutation

   Promoter

   L30

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO-Cre sgAmotl2 v3

    Plasmid

    #158602

    Purpose

    lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Amotl2 sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

    Plasmid

    #217513

    Purpose

    Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

    Depositor

    S. Lawrence Zipursky

    Article

    Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

    Insert

    10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. JN302: pMVP/SB/Puro-DEST

    Plasmid

    #121865

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPbcsx28

    Plasmid

    #89909

    Purpose

    Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx28
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBM019

    Plasmid

    #128179

    Purpose

    Construct for generating stable Cas9-expressing Toxoplasma gondii

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Inserts

    sgRNA#1
    SpCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Expression

    Mutation

    Promoter

    TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

    Plasmid

    #176745

    Purpose

    Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Glt1-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAG/hTAZ-FL

    Plasmid

    #139979

    Purpose

    Expresses full length human Tafazzin with codon modified for cloning. Transgene flanked by AAV2 ITR

    Depositor

    William Pu

    Article

    Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

    Insert

    Full length human Tafazzin (TAFAZZIN Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFK210B-AtMIR390a-B/c

    Plasmid

    #51777

    Purpose

    Plant expression vector (35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMDC32B-AtTAS1c-B/c

    Plasmid

    #51773

    Purpose

    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtTAS1c-B/c
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LC101: pMVP/PB/Neo-DEST

    Plasmid

    #121875

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMDC123SB-AtTAS1c-B/c

    Plasmid

    #51772

    Purpose

    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtTAS1c-B/c
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK5RS-HERCD533

    Plasmid

    #65224

    Purpose

    Expression of dominant negative EGFR in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

    Insert

    HERCD533 (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted AA 654-1186

    Promoter

    CMV

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

22. pTC-ApoE-Tet-YAP

    Plasmid

    #85580

    Purpose

    Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver

    Depositor

    Ursula Ehmer

    Article

    Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

    Inserts

    CreER
    YAP
    

    Use

    Cre/Lox

    Tags

    Flag (2x)

    Expression

    Mammalian

    Mutation

    Promoter

    ApoE.HCR.hAAT and TRE

    Available since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEIAV-DIO-TLoop-CHR2YFP

    Plasmid

    #66798

    Purpose

    expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector

    Depositor

    Edward Callaway

    Article

    Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

    Insert

    Floxed Chr2-YFP
    

    Use

    Lentiviral

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CMVTetO7 Promoter

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mutation

    Promoter

    TgTUB1

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA

    Plasmid

    #176743

    Purpose

    Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Cx43-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid

    #84730

    Purpose

    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Use

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR-AtTAS1c-B/c

    Plasmid

    #51774

    Purpose

    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtTAS1c-B/c
    

    Use

    Gateway-compatible entry clone

    Tags

    Expression

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBSFI-myr-akt-K179M

    Plasmid

    #49188

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    K179M

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

    Plasmid

    #72888

    Purpose

    fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Insert

    Gaussia luciferase, Channelrhodopsin-2, EYFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUB-nSypHer (NLS:sypHer:NLS)

    Plasmid

    #84731

    Purpose

    Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    nSypHer
    

    Use

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBZcsx27

    Plasmid

    #89900

    Purpose

    Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx27
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLV sgRNA-Notch1 #1 GFP

    Plasmid

    #106950

    Purpose

    Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    gRNA targeting Notch1 (Notch1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pWPXL-Hes1

    Plasmid

    #36983

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Hes1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pH7WG2B-OsMIR390-B/c

    Plasmid

    #61465

    Purpose

    Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    OsUBI

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

    Plasmid

    #32483

    Purpose

    Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    ChR2HA-2a-PSAML141F,Y115F:GlyR
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    ChR2 2A

    Expression

    Mutation

    Promoter

    CAG

    Available since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBSFI-myr-akt-S473A

    Plasmid

    #49190

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    S473A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

38. pROF402

    Plasmid

    #155334

    Purpose

    Binary vector for expression of FLuc controlled by the blue light-repressible system 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pROF441

    Plasmid

    #155336

    Purpose

    Binary vector for expression of a gRNA targeting AtAP1 promoter 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pY005 (pcDNA3.1-hLb3Cpf1)

    Plasmid

    #69977

    Purpose

    Expresses humanized Lb3Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb3Cpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

41. pET28a-MSP1D1deltaH4/2H5

    Plasmid

    #71715

    Purpose

    Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAAV hSyn1 ChR2 ET-TC 2A tDimer

    Plasmid

    #101361

    Purpose

    High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Inserts

    Channelrhodopsin-2
    synaptophysin-red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E123T , T159C

    Promoter

    human Synapsin

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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43. pXPR_BRD003 sgKEAP1-2

    Plasmid

    #74986

    Purpose

    sgKEAP1-2, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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44. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. pET28a-MSP1D1deltaH4/2

    Plasmid

    #71712

    Purpose

    Membrane scaffold protein (MSP), half of helix 4 truncated

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBSFI-myr-akt-T308A

    Plasmid

    #49189

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

47. pSKA397

    Plasmid

    #80380

    Purpose

    mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    cat
    metE
    

    Use

    Synthetic Biology; Mini-tn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat promoter and cpcG2Δ59

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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49. pMDC32B-OsMIR390-B/c

    Plasmid

    #61467

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

    Depositor

    James Carrington

    Article

    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

    Insert

    OsMIR390-B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    O. sativa MIR390 precursor sequence including a c…

    Promoter

    2x35S

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP

    Plasmid

    #114378

    Purpose

    expresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-KA2N-EGFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EGFP and KA2N

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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