Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

Your Selections

20+ requests

19,178 results

Showing: 1621 - 1650 of 19178 results

  1. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73797-plasmid-map-sequence-id-196924

    Enlarge

  3. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

    Enlarge

  19. pCAG-NLS-HA-Bxb1

    Plasmid
    #51271
    Purpose
    Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    NLS-HA-Bxb1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51271-plasmid-map-sequence-id-386475

    Enlarge

  20. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid
    #60229
    Purpose
    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60229-plasmid-map-sequence-id-419029

    Enlarge

  22. lenti-sgRNA blast

    Plasmid
    #104993
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104993-plasmid-map-sequence-id-342099

    Enlarge

  23. pCMV-PE6c

    Plasmid
    #207853
    Purpose
    Mammalian expression of PE6c prime editor
    Depositor
    David Liu
    Article
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Insert
    PE6c
    Use
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207853-plasmid-map-sequence-id-423164

    Enlarge

  29. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid
    #167288
    Purpose
    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.
    Depositor
    Agnel Sfeir
    Article
    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:
    Insert
    DDX58 (RIGI Human)
    Use
    Lentiviral
    Tags
    APEX2 and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167288-plasmid-map-sequence-id-330940

    Enlarge

  30. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Use
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

    Enlarge

Showing: 1621 - 1650 of 19178 results