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pCAG-CreERT2
Plasmid#14797PurposeMammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)DepositorInsertCre-ERT2 fusion protein
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEN-luxCDABE
Plasmid#44918DepositorInsertsigma 70 promoter
UseLuciferaseTagslux transcriptional fusionExpressionMutationPromoterem7Available sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pmScarlet-i_C1
Plasmid#85044PurposeMammalian expression of mScarlet-iDepositorHas ServiceCloning Grade DNAInsertmScarlet-i
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_Donor (EMM0021)
Plasmid#63934Purpose3X Flag with P2A-Neo for 3' tagging of endogenous proteinsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsGlycine-Serine-3XFlag-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoTPNOX
Plasmid#87854PurposeThis plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.DepositorInsertmitoTPNOX
UseTagsC-terminal FLAG tag with a linker and mitochondri…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-TFE3
Plasmid#38120DepositorInsertTFE3 (TFE3 Human)
UseTagsEGFPExpressionMammalianMutationdeleted stop codonPromoterCMVAvailable sinceJuly 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-M2L
Plasmid#20926Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28DepositorArticleUseEpisomal/ebnaTagsExpressionMutationPromoterCMV and IRESAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBAC-lacZ
Plasmid#13422DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-MCS
Plasmid#80488Purposedonor vector for AAVS1 targeting (puromycin selection) with multiple cloning siteDepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
PH-Akt-GFP
Plasmid#51465PurposeIs a biosensor for PIP3/PI(3,4)P2DepositorInsertAKT (aa 1–164)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-P-CAG-DEST
Plasmid#80490PurposeGateway Destination donor vector for AAVS1 targeting (puromycin selection)DepositorTypeEmpty backboneUseDonor vector (human)TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-3XmCherry
Plasmid#64108PurposeExpression of Sp dCas9-3XmCherry in mammalian cellsDepositorInsertSp dCas9
UseCRISPR and LentiviralTags3XmCherryExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEP4 E02S ET2K
Plasmid#20927Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4DepositorArticleUseEpisomal/ebnaTagsExpressionMutationPromoterAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
BPK764
Plasmid#65767PurposeBacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNADepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
UseCRISPRTags3x FLAG and NLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFast-FT-N1
Plasmid#31910DepositorInsertFast-FT
UseTagsExpressionMammalianMutationM18V/E34K/K69R/L84W/S149T/A224S compared to mCher…PromoterCMVAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-3XGFP
Plasmid#64107PurposeExpression of Sp dCas9-3XGFP in mammalian cellsDepositorInsertSp dCas9
UseLentiviralTags3XsfGFPExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Rosa26 mT/mG
Plasmid#17787DepositorInsertRosa26 mT/mG
UseCre/Lox and Mouse TargetingTagsmembrane tagExpressionMammalianMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only
