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161,202 results

Showing: 2951 - 3000 of 161202 results

  2. pDule-pCNF

    Plasmid
    #85494
    Purpose
    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62
    Insert
    para-cyanophenylalanine Mj synthetase
    Use
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32L L65V F108W Q109M D158G I159P
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85494-plasmid-map-sequence-id-169861

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  4. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid
    #139989
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139989-plasmid-map-sequence-id-275392

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  6. pDV002

    Plasmid
    #191823
    Purpose
    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR
    Depositor
    Jim Roberts
    Article
    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    191823-plasmid-map-sequence-id-375876

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  15. TFORF2536

    Plasmid
    #142397
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX6 (SOX6 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    142397-plasmid-map-sequence-id-454976

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  18. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid
    #26976
    Purpose
    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation
    Depositor
    Karl Deisseroth
    Has Service
    AAV5, AAV8, and AAV9
    Insert
    hChR2(H134R)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    hSyn
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26976-plasmid-map-sequence-id-313556

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  28. pBS31-cCARLIN

    Plasmid
    #160928
    Purpose
    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)
    Depositor
    Fernando Camargo
    Article
    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.
    Insert
    CARLIN gRNAs and CAG-GFP-CARLIN array
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter for gRNAs; CAG promoter for GFP
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160928-plasmid-map-sequence-id-421629

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  29. GPR120-Tango

    Plasmid
    #66310
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR120 (FFAR4 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66310-plasmid-map-sequence-id-248122

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  36. pNeae2

    Plasmid
    #168300
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:
    Insert
    Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
    Use
    Tags
    6xHis, E-tag, and myc-tag
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168300-plasmid-map-sequence-id-334450

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  44. pKAR2-Br512

    Plasmid
    #161875
    Purpose
    Expresses Br512 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Maranhao et al medRxiv
    Insert
    Modified DNA Polymerase I Large Fragment (Br512)
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (HP47)…
    Expression
    Bacterial
    Mutation
    Promoter
    T7, tetPA
    Available since
    Nov. 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    161875-plasmid-map-sequence-id-316881

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Showing: 2951 - 3000 of 161202 results