We narrowed to 24,790 results for: SPR

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PX458_ZBTB21_2

Plasmid

#135742

Purpose

Encodes gRNA for 3' target of human ZBTB21

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB21 gRNA (ZBTB21 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZNF264_iso1_1

Plasmid

#135748

Purpose

Encodes gRNA for 3' target of human ZNF264_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF264_iso1 gRNA (ZNF264 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZNF503_1

Plasmid

#135754

Purpose

Encodes gRNA for 3' target of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 gRNA (ZNF503 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Mutation

K855A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-sgRNA-M5-IRES-CFP

Plasmid

#114728

Purpose

sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF792_2

Plasmid

#86340

Purpose

Encodes gRNA for 3' target of human ZNF792

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF792 (ZNF792 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZSCAN9_1

Plasmid

#86312

Purpose

Encodes gRNA for 3' target of human ZSCAN9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZSCAN9 (ZNF193 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_SP5_1

Plasmid

#86362

Purpose

Encodes gRNA for 3' target of human SP5

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SP5

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZFP1_iso1_2

Plasmid

#86361

Purpose

Encodes gRNA for 3' target of human ZFP1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZFP1_iso1 (ZFP1 Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZFP1_iso1_1

Plasmid

#86360

Purpose

Encodes gRNA for 3' target of human ZFP1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZFP1_iso1 (ZFP1 Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF792_1

Plasmid

#86339

Purpose

Encodes gRNA for 3' target of human ZNF792

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF792 (ZNF792 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MIXL1_2

Plasmid

#86336

Purpose

Encodes gRNA for 3' target of human MIXL1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIXL1 (MIXL1 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MIXL1_1

Plasmid

#86335

Purpose

Encodes gRNA for 3' target of human MIXL1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIXL1 (MIXL1 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF580_2

Plasmid

#86332

Purpose

Encodes gRNA for 3' target of human ZNF580

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF580 (ZNF580 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF580_1

Plasmid

#86331

Purpose

Encodes gRNA for 3' target of human ZNF580

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF580 (ZNF580 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZSCAN9_2

Plasmid

#86313

Purpose

Encodes gRNA for 3' target of human ZSCAN9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZSCAN9 (ZNF193 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_SAP130_2

Plasmid

#86311

Purpose

Encodes gRNA for 3' target of human SAP130

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against SAP130 (SAP130 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x5

Plasmid

#63605

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PBAD-sgRNA-VR

Plasmid

#62286

Purpose

expression of VR sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

VR sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA:Tyr (albino)

Plasmid

#60966

Purpose

Expression vector of rat Tyr (albino) guide RNA

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

Tyrosinase gRNA

Use

CRISPR

Promoter

T7

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
pT7-gRNA:Tyr (wild)

Plasmid

#60967

Purpose

Expression vector of rat Tyr (wild) guide RNA

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

Tyrosinase gRNA

Use

CRISPR

Available Since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
MsMUT_sgRNA1

Plasmid

#111296

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA1 against murine MUT

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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MsMUT_sgRNA3

Plasmid

#111297

Purpose

CRISPR KO murine MUT

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

sgRNA3 against murine MUT

Use

CRISPR and Lentiviral

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pJK384.4

Plasmid

#155369

Purpose

dCas9 + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas9 (bacteria)
PA4-mVenus

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Availability

Academic Institutions and Nonprofits only

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pdCas9-bacteria

Plasmid

#44249

Purpose

aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 (bacteria)

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

pLtetO-1

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

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pFnCpfAb-apr

Plasmid

#214198

Purpose

Expresses FnCpf1 and RecAb recombination system for genome editing in Acinetobacter baumannii

Depositor

Yu Wang

Article

Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

Insert

FnCpf1

Use

CRISPR

Promoter

tac promoter

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQAT48

Plasmid

#223420

Purpose

T-DNA vector for Mb2Cas12a-RVRR based mutagenesis for dicot plants; more relaxed PAM requirements; Mb2Cas12a-RVRR and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-Mb2Cas12a-RVRR-2x35s-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCasAb-apr

Plasmid

#121998

Purpose

Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLX_311-KRAB-dCas9

Plasmid

#96918

Purpose

for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

Depositor

John Doench, William Hahn, David Root

Article

Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

Insert

dCas9

Use

Lentiviral

Tags

KRAB

Expression

Mammalian

Mutation

dCas9

Promoter

EF1a

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pXR003: CasRx gRNA cloning backbone

Plasmid

#109053

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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VPR_dCas9

Plasmid

#134601

Purpose

Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

Depositor

Lasse Ebdrup Pedersen

Article

Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

Insert

VPR-dCas9-2A-ZsGreen-DR

Tags

VPR and ZsGreen1-DR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-VP64_Blast

Plasmid

#192650

Purpose

3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-VPR

Plasmid

#191269

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

VPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-KRAB

Plasmid

#191314

Purpose

piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

KRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBC-MT1T2

Plasmid

#50593

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T1, gRNA scaffold, OsU3t plus, TaU3p, T2

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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We narrowed to 24,790 results for: SPR