4,146 results

1. FUCas9Cherry

   Plasmid

   #70182

   Purpose

   Expresses Cas9 with fluorescent Cherry reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   humanised S.pyogenes cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSRE SSG

   Plasmid

   #127692

   Purpose

   Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Structural Genome
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pAAV-TRE-EGFP

   Plasmid

   #89875

   Purpose

   EGFP reporter vector of iTango system for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   tetO-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2-MT GLI2 FL

   Plasmid

   #17648

   Depositor

   Erich Roessler

   Article

   Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

   Insert

   GLI2 (GLI2 Human)
   

   Use

   Tags

   6x Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Ptet-RFP-shR-rtTA

   Plasmid

   #35625

   Purpose

   Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

   Depositor

   Howard Gu

   Article

   Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

   Type

   Empty backbone

   Use

   AAV and RNAi; Tet inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ptet

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3β-FLAG-CBP-HA

   Plasmid

   #32908

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   CBP (Crebbp Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCI-SEP-GluR1

   Plasmid

   #24000

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR1 (Gria1 Rat)
   

   Use

   Tags

   GluR1 signal peptide (aa1-22) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 GFP LIC cloning vector (6D)

   Plasmid

   #30127

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAGIG

   Plasmid

   #11159

   Purpose

   Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBABEpuro HA Brca1

    Plasmid

    #14999

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

    Insert

    Brca1 (BRCA1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-Ub KO.G76V

    Plasmid

    #11932

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin KO.G76V (RBX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Conjugation deficient mutant. All 7 lysines of ub…

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCerulean3-C1

    Plasmid

    #54605

    Purpose

    Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Type

    Empty backbone

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. dCas9 plasmid

    Plasmid

    #100091

    Purpose

    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFlag-NLS-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCCL-CellCycle

    Plasmid

    #132429

    Purpose

    Expressing Fucci cell cycle indicator in mammalian cells

    Depositor

    John M. Greally

    Article

    Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

    Insert

    hCdt, hGeminin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABEpuro-gateway

    Plasmid

    #51070

    Purpose

    gateway adapted mammalian expression vector

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1 EYFP H148Q/I152L

    Plasmid

    #25872

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q/I152L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with H148Q and I152L m…

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLEM415-ldhL-mRFP1

    Plasmid

    #99842

    Purpose

    Expression of RFP in Lactobacillus

    Depositor

    Sujin Bao

    Article

    Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

    Insert

    monomeric red fluorescent protein
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    ldhl

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Slug_pGL2

    Plasmid

    #31695

    Depositor

    Paul Wade

    Article

    Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

    Insert

    Slug (SNAI2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ES-FUCCI

    Plasmid

    #62451

    Purpose

    SMX_DEST vector with 3 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
    minPGK::Cherry-hCdt1(30-120)-bGHpA
    PGK::HygroR-bGpA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. human EB1 fused to monomeric RFP

    Plasmid

    #39323

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Inserts

    EB1 (MAPRE1 Human)
    mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pICH86966::AtU6p::sgRNA_PDS

    Plasmid

    #46966

    Purpose

    Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    AtU6p::sgRNA_PDS
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFPC1-huNFATc1EE-WT

    Plasmid

    #24219

    Depositor

    Jerry Crabtree

    Article

    Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    EE and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EF.STAT3DN.Ubc.GFP

    Plasmid

    #24984

    Purpose

    Depositor

    Linzhao Cheng

    Article

    Hillion et al Cancer Research 68, 10121, December 2008

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y705F--dominant negative

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PX551

    Plasmid

    #60957

    Purpose

    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

    Depositor

    Feng Zhang

    Article

    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    pMecp2

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-hAR

    Plasmid

    #89078

    Purpose

    mammalian expression of human androgen receptor in pCMV5

    Depositor

    Elizabeth Wilson

    Article

    Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

    Insert

    human androgen receptor (hAR) full-length (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Use

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMXs-puro GFP-p62

    Plasmid

    #38277

    Depositor

    Noboru Mizushima

    Article

    Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1 mycBioID

    Plasmid

    #35700

    Purpose

    To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    cmv

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. FUW-OSKM

    Plasmid

    #20328

    Purpose

    Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge