4,136 results

1. pMKO.1 puro

   Plasmid

   #8452

   Purpose

   Retroviral vector for shRNA expression.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Type

   Empty backbone

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA 3 5' cMyc SMC1 wt

   Plasmid

   #32363

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Use

   Tags

   c-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag-HA-USP11

   Plasmid

   #22566

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-C1-CAMKIIalpha

   Plasmid

   #21226

   Depositor

   Tobias Meyer

   Article

   Shen et al Neuron. 1998 Sep . 21(3):593-606.

   Insert

   CAMKIIalpha (Camk2a Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lab plasmid KS 0001.

   Promoter

   Available since

   July 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. eIF2a 2

   Plasmid

   #21808

   Depositor

   David Ron

   Insert

   eIF2a (EIF2S1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S52A

   Promoter

   Available since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

   Plasmid

   #141374

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pCMVHA SUZ12

   Plasmid

   #24232

   Purpose

   Mammalian expression of human SUZ12 with HA tag

   Depositor

   Kristian Helin

   Article

   Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. Puro.Cre empty vector

   Plasmid

   #17408

   Purpose

   Lentiviral expression of Cre from the mouse PGK promoter

   Depositor

   Tyler Jacks

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

   Insert

   Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse PGK

   Available since

   March 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

10. MSCV JMJD3 mutant

    Plasmid

    #21214

    Depositor

    Paul Khavari

    Article

    Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1390A

    Promoter

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTriEx4-Rac1-2G

    Plasmid

    #66110

    Purpose

    Second generation Rac1 FRET biosensor

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGal4AD-CIB1

    Plasmid

    #28245

    Purpose

    Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    Gal4AD (CIB1, GAL4 Budding Yeast)
    

    Use

    Tags

    CIB1

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid

    #103870

    Purpose

    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-GAL4

    Plasmid

    #24345

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-Azurite

    Plasmid

    #36086

    Purpose

    3rd generation lentiviral plasmid

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGH335_MS2-AID*Δ-Hygro

    Plasmid

    #85406

    Purpose

    lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AID*Δ and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBI-EGFP-MnSOD

    Plasmid

    #16612

    Depositor

    Bert Vogelstein

    Article

    Hwang et al Nat Med. 2001 Oct . 7(10):1111-7.

    Insert

    MnSOD (SOD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCFJ420 - Peft-3::GFP::H2B (ubiquitous)

    Plasmid

    #34877

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Peft-3 GFP H2B tbb-2 UTR
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    S65C

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Feb. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCitrine-N1

    Plasmid

    #54594

    Purpose

    Localization: N1 Cloning Vector, Excitation: 516, Emission: 529

    Depositor

    Michael Davidson, Roger Tsien, Oliver Griesbeck, Robert Campbell

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Type

    Empty backbone

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pET11phiC31poly(A)

    Plasmid

    #18942

    Depositor

    Michele Calos

    Article

    Groth et al Genetics. 2004 Apr . 166(4):1775-82.

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAVS1-P-CAG-DEST

    Plasmid

    #80490

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Tailless VinculinTS

    Plasmid

    #26020

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    tailless vinculinTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid

    #103871

    Purpose

    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-TO-nmdCas9-3XmCherry

    Plasmid

    #64110

    Purpose

    Expression of Nm dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-SpyTagMBP

    Plasmid

    #35050

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyTagMBP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Simple peptide tag added to the N-terminus of MBP…

    Promoter

    T7 promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-C1-hAtg2A

    Plasmid

    #36456

    Depositor

    Noboru Mizushima

    Article

    Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

    Insert

    autophagy-related protein 2 homolog A (ATG2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Proline 656 to Arginine

    Promoter

    CMV

    Available since

    May 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMSCV-loxp-dsRed-loxp-3xHA-Puro-WPRE

    Plasmid

    #32703

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Cre/Lox and Retroviral

    Tags

    3x HA tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGADCg

    Plasmid

    #20161

    Depositor

    Peter Uetz

    Article

    Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Gal4 AD and SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-double floxed-eNpHR-EYFP-WPRE-pA

    Plasmid

    #20949

    Purpose

    Cre-activated AAV expression of halorhodopsin 2.0 (eNpHR) fused to EYFP for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    halorhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET-15b-TEV-C9R

    Plasmid

    #118754

    Purpose

    TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

    Depositor

    Yutaka Kuroda

    Article

    Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

    Insert

    Tobacco Etch Virus protease (NEWENTRY Synthetic)
    

    Use

    Tags

    C9R and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. AAV-CAGGS-EGFP

    Plasmid

    #22212

    Purpose

    AAV zinc finger donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    CAGGS-eGFP
    

    Use

    Zfn donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid

    #68375

    Purpose

    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK1

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid

    #141183

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    

    Use

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. TetR in pENTR1A (559-1)

    Plasmid

    #22265

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor (tetR )
    

    Use

    Tags

    Expression

    Insect, Mammalian, Worm Exp…

    Mutation

    Promoter

    Available since

    Dec. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3-TORCAR(T/A)

    Plasmid

    #64928

    Purpose

    Negative-control mutant for TORCAR biosensor.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR(T/A) (EIF4EBP1 Synthetic, Human)
    

    Use

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Mutation

    4EBP1 domain contains T37A and T46A mutations.

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK7-HA-S6K1-WT

    Plasmid

    #8984

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. IgK-IFN-luc

    Plasmid

    #14886

    Purpose

    Reporter construct for NF‐κB activation. Contains two copies of immunoglobulin kappa light chain motif (5′‐GGGGACTTTCC‐3′) upstream of the a minimal promoter driving luciferase expression

    Depositor

    David Baltimore

    Article

    Pomerantz et al EMBO J. 2002 Oct 1. 21(19):5184-94.

    Insert

    IgK-IFN (IFNB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1 Flag Runx1 FL (P#1790)

    Plasmid

    #14585

    Depositor

    Matija Peterlin

    Article

    Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUC19-HRP-C

    Plasmid

    #40163

    Depositor

    Paul Ortiz de Montellano

    Article

    Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

    Insert

    Horseradish peroxidase
    

    Use

    Coli cloning vector

    Tags

    Six histidine

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-ccdB-3xFLAG-V5

    Plasmid

    #87063

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge