Skip to main content
Addgene
Showing: 2761 - 2800 of 4070 results

  4. pET His6 NusA TEV LIC cloning vector (1N)

    Plasmid
    #29657
    Depositor
    Scott Gradia
    Type
    Empty backbone
    Use
    Tags
    6xHis, NusA, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    Aug. 17, 2011
    Availability
    Academic Institutions and Nonprofits only
    29657-plasmid-map-sequence-id-418922

    Enlarge

  9. pFGC-pcoCas9

    Plasmid
    #52256
    Purpose
    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA
    Depositor
    Jen Sheen
    Insert
    35SPPDK:pcoCas9:NOS cassette
    Use
    CRISPR; Plant expression
    Tags
    Expression
    Mutation
    Promoter
    constitutive 35SPPDK promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    52256-plasmid-map-sequence-id-116013

    Enlarge

  13. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid
    #20298
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20298-plasmid-map-sequence-id-191904

    Enlarge

  19. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid
    #62723
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Has Service
    AAV1 and AAV5
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62723-plasmid-map-sequence-id-166279

    Enlarge

  26. pAAV-CaMKIIa-hM3D(Gq)-mCherry

    Plasmid
    #50476
    Purpose
    Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM3D(Gq)-mCherry (CHRM3 Human)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50476-plasmid-map-sequence-id-171073

    Enlarge

  29. pDONR223 SARS-CoV-2 NSP16

    Plasmid
    #141269
    Purpose
    Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141269-plasmid-map-sequence-id-288092

    Enlarge

  33. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

    Enlarge

Showing: 2761 - 2800 of 4070 results