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161,273 results

Showing: 3451 - 3500 of 161273 results

  1. TRUPATH Triple Gaq

    Plasmid
    #196058
    Purpose
    Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaQ-RLuc8 (GNAQ Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196058-plasmid-map-sequence-id-385868

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  3. pUC57-CmYLCV

    Plasmid
    #205245
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    CmYLCV
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205245-plasmid-map-sequence-id-408849

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  6. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51083
    Purpose
    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51083-plasmid-map-sequence-id-175189

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  8. ePPEplus

    Plasmid
    #205241
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205241-plasmid-map-sequence-id-408831

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  10. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

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  15. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid
    #229678
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    monomeric StayGold, HA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229678-plasmid-map-sequence-id-457125

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  19. pDG02583

    Plasmid
    #104129
    Purpose
    Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    NEDD8-specific protease 1 (NEDP1)
    Use
    Tags
    14x Histidine tag, Maltose binding protein, and S…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104129-plasmid-map-sequence-id-313426

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  20. (+)-Limonene Synthase

    Plasmid
    #112230
    Purpose
    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.
    Depositor
    Daniel Oprian
    Article
    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17
    Insert
    (+)-Limonene Synthase
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-52
    Promoter
    Available since
    June 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    112230-plasmid-map-sequence-id-214310

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  25. AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781)

    Plasmid
    #199777
    Purpose
    enhancer AAV for Cre recombinase expression in striatal indirect pathway D2-MSNs
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Has Service
    AAV PHP.eB
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mutation
    R297T for reduced recombinase efficiency.
    Promoter
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    199777-plasmid-map-sequence-id-391721

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  28. pRPF215

    Plasmid
    #106377
    Purpose
    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon
    Depositor
    Neil Fairweather, Robert Fagan
    Article
    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1
    Insert
    Ptet-himar1-Ter(slpA)-transposon
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Codon optimised for C. difficile
    Promoter
    Ptet
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    106377-plasmid-map-sequence-id-205135

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  33. pN7HELIX

    Plasmid
    #190662
    Purpose
    Expresses polycistronic N7 HELIX where Cas12k-T2A-TnsC and TniQ-T2A-nAniI_TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, nAniI_N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190662-plasmid-map-sequence-id-385664

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  34. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid
    #188385
    Purpose
    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188385-plasmid-map-sequence-id-371050

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  36. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  45. iLID-mCl3-MAP7N

    Plasmid
    #190167
    Purpose
    Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.
    Depositor
    Anna Akhmanova
    Article
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Insert
    iLID-mClover3-MAP7N
    Use
    Tags
    iLID-mClover3
    Expression
    Mammalian
    Mutation
    aa 1-309 from human MAP7 delta175-213
    Promoter
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    190167-plasmid-map-sequence-id-375649

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  50. p189

    Plasmid
    #65207
    Purpose
    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3451 - 3500 of 161273 results