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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Publication

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

71 Articles

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EBFP2-C1

Plasmid

#54665

Purpose

Localization: C1 Cloning Vector, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Type

Empty backbone

Tags

EBFP2

Expression

Mammalian

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pCI-EGFP-NR1 wt

Plasmid

#45446

Depositor

Andres Barria, Robert Malinow

Publication

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR1 (Grin1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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shYAP1 # 1

Plasmid

#42540

Depositor

William Hahn

Publication

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pLV-beta-catenin deltaN90

Plasmid

#36985

Depositor

Bob Weinberg

Publication

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

beta-catenin deltaN90 (CTNNB1 Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Deletion of amino acids 1-90 (Constitutively Acti…

Promoter

EF1a

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDest-eGFP-N1

Plasmid

#31796

Depositor

Robin Shaw

Publication

Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pcDNA3-Myc-IQGAP1

Plasmid

#30118

Depositor

David Sacks

Publication

Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Insert

IQGAP1 (IQGAP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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EGFPC1-huNFATc1EE-WT

Plasmid

#24219

Depositor

Jerry Crabtree

Publication

Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

Insert

NFATc1 (NFATC1 Human)

Tags

EE and GFP

Expression

Mammalian

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pEF5B-FRT-GFP-αTAT1

Plasmid

#27099

Depositor

Maxence Nachury

Publication

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

α-Tubulin K40 acetyltransferase1 (ATAT1 Human)

Tags

GFP, S-tag, and TEV site

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pLenti CMV/TO Zeo DEST (644-1)

Plasmid

#17294

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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FLAG-Foxo1(pCMV5)

Plasmid

#12148

Depositor

Domenico Accili

Publication

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 (Foxo1 Mouse)

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pEGFP-N1-STAT3

Plasmid

#111934

Purpose

Expresses human STAT3 fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Publication

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Signal transducer and activator of transcription 3 (STAT3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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JAK2 (V617F)-pcw107-V5

Plasmid

#64610

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

JAK2 (JAK2 Human)

Use

Lentiviral

Tags

V5

Mutation

V617F

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pZac2.1 gfaABC1D-cyto-GCaMP6f

Plasmid

#52925

Purpose

Fluorescent reporter for calcium signaling

Depositor

Baljit Khakh

Publication

Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

Has service

AAV5

Insert

GCaMP6f

Use

AAV

Available since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

53 Articles

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mEmerald-C1

Plasmid

#53975

Purpose

Localization: C1 Cloning Vector, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Type

Empty backbone

Tags

mEmerald

Expression

Mammalian

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

11 Articles

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pEGFP-N1 human cofilin WT

Plasmid

#50859

Purpose

expresses human cofilin fused to EGFP in mammalian cells

Depositor

James Bamburg

Publication

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pRK5-myc-Miro1

Plasmid

#47888

Purpose

Expresses myc tagged Miro1

Depositor

Pontus Aspenström

Publication

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 (RHOT1 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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CMV-NLS-R-GECO

Plasmid

#32462

Purpose

Mammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Tags

NLS sequence DPKKKRKV

Expression

Mammalian

Mutation

Substitutions relative to the mApple-derived anal…

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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CMV-G-GECO1.0

Plasmid

#32447

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.0

Expression

Mammalian

Mutation

GCaMP3 K119I/L173Q/S404G/E430V

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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EGFP-Rab7A T22N

Plasmid

#28048

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Mutation

T22N

Available since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pEF1-alpha-V

Plasmid

#27290

Depositor

Timothy Springer

Publication

Takagi et al Cell. 2002 Sep 6. 110(5):599-11.

Insert

integrin alpha V (ITGAV Human)

Expression

Mammalian

Mutation

Isoform 2

Available since

March 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3.2/v5-DEST hGlut1

Plasmid

#18085

Depositor

Wolf Frommer

Publication

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut1 (SLC2A1 Human)

Expression

Mammalian

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-CaMKIIa-eNpHR 3.0-EYFP

Plasmid

#26971

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Has service

AAV1 and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking Signal (TS) ER Export Signal

Promoter

CaMKIIa

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pCMV/SB11

Plasmid

#26552

Depositor

Perry Hackett

Publication

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Mutation

Methionine 243 to Glutamine Threonine 136 to Arg…

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Flag-HA-GFP

Plasmid

#22612

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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Flag-HA-USP14

Plasmid

#22569

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP14 (USP14 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pKP332 (Lenti-OSK1)

Plasmid

#21627

Depositor

Tim Townes

Publication

Chang et al Stem Cells. 2009 May . 27(5):1042-9.

Insert

hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Human, Porcine teschovirus-1)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pK-myc-Par3b

Plasmid

#19388

Depositor

Ian Macara

Publication

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 3b (PARD3 Human)

Tags

Myc

Expression

Mammalian

Mutation

Full length, isoform b.

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pGRG25

Plasmid

#16665

Depositor

Nancy Craig

Publication

McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

Insert

mTn7::MCS

Expression

Bacterial

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLNCX chick src Y527F

Plasmid

#13660

Depositor

Joan Brugge

Insert

src Y527F (SRC Chicken)

Use

Retroviral

Expression

Mammalian

Mutation

Y527F. Constitutive active mutant.

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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