8,885 results
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psfTn5
Plasmid#79107Purposebacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutationsDepositorInserttransposase 5
TagsHisExpressionBacterialMutationE54K and L372PAvailable SinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP_a_tubulin_C1
Plasmid#21039DepositorAvailable SinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA 3 5' cMyc SMC1 wt
Plasmid#32363DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM-LIN28
Plasmid#16350DepositorInsertHomo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
ExpressionBacterialAvailable SinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
Tags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
FR_HNF1B
Plasmid#31101DepositorAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-H2B Control Plasmid
Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsSNAP-tag (SNAPf)ExpressionMammalianPromoterCMVAvailable SinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBMN(CMV-copGFP-Puro-SMAR)
Plasmid#80391Purposeexpression copGFP and Puromycine, with an S/MAR element for episomal maintainceDepositorInsertcopGFP-T2A-Puromycine
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-RB1
Plasmid#87836PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.DepositorInsertsgRB1 (RB1 Human)
UseCRISPR and Lentiviral; Doxycycline inducible; egf…ExpressionMammalianPromoterTight TRE promoter and U6Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-CBE C-terminal
Plasmid#137176PurposeAAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNADepositorInsertv5 AAV-CBE C-terminal; U6-protospacer
UseAAVPromoterCbhAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.LifeAct-Ruby.W
Plasmid#51009PurposeLentivector that expresses LifeAct-Ruby from internal human PGK promoterDepositorInsertLifeAct-mRFPruby
UseLentiviralTagsLifeActExpressionMammalianPromoterhuman PGKAvailable SinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-Cas9-2A-GFP-sgRNA
Plasmid#124889PurposeContains spCas9-2A-GFP and guide RNA scaffold in single retroviral vectorDepositorInsertCas9
UseRetroviralTags2A-GFPPromoterMSCVAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-p105
Plasmid#23288DepositorInsertp105 (NFKB1 Human)
ExpressionMammalianAvailable SinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV6M-Pak1
Plasmid#12209DepositorAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HIF1 alpha
Plasmid#21101DepositorInsertHYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
ExpressionMammalianAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TFR-20
Plasmid#55144PurposeLocalization: Transferrin Receptor, Excitation: 587, Emission: 610DepositorAvailable SinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG-gate-FLAG-IRES-GFP
Plasmid#107398PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsFLAGExpressionMammalianAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSin-EF2-LIN28-Pur
Plasmid#16580DepositorAvailable SinceJan. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP8
Plasmid#22608DepositorAvailable SinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-BlaM-Vpr
Plasmid#21950DepositorInsertBlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
ExpressionMammalianAvailable SinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
Murine E-cadherin mCherry
Plasmid#71366Purposemammalian expression of murine E-caderin with mCherry fusionDepositorAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
EX-HA-FRB-Cb5_pLX304
Plasmid#120915PurposeLentiviral vector expressing EX-HA-FRB-Cb5 on mammalian ER membraneDepositorInsertEX-HA-FRB-Cb5
UseLentiviralExpressionMammalianAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
plenti-CAG -IRES-GFP
Plasmid#69047PurposeLentiviral backbone for GFP coexpressionDepositorTypeEmpty backboneUseLentiviralTagsIRES-GFPExpressionMammalianAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-cMyc
Plasmid#46970Purposelentiviral expression of c-MycDepositorAvailable SinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-HA-hUBE1L
Plasmid#12438DepositorAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
V5-APEX2-LMNA
Plasmid#129275PurposeExpression of APEX2 at nuclear laminaDepositorInsertV5-APEX2-LMNA
UseLentiviralExpressionMammalianPromoterCMV promoterAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
psd44-GqQL
Plasmid#46826Purpose2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cellsDepositorInsertg protein alpha q (GNAQ Human)
UseLentiviralExpressionMammalianMutationQ209L (The mutation reduces GTPase activity resul…PromoterPGK promoterAvailable SinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-GB
Plasmid#60520PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc3-HDM2
Plasmid#20935DepositorAvailable SinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
PL-SIN-Oct4-EGFP
Plasmid#21319PurposeLentiviral vector, expresses EGFP under Oct3/4 promoterDepositorInsertEnhanced Green Fluorescence Protein
UseLentiviralExpressionMammalianPromoterOct-4 promoter regionAvailable SinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-MCS
Plasmid#84008Purposedoxycycline-inducible lentiviral vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralExpressionMammalianPromoterTRE-CMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-TEV-C9R
Plasmid#118754PurposeTEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activityDepositorInsertTobacco Etch Virus protease (NEWENTRY Synthetic)
TagsC9R and His tagExpressionBacterialPromoterT7Available SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-Nrxn1beta AS4(-)
Plasmid#59409PurposeExpression plasmid for HA-tagged mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]DepositorAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSecUAG-Evol2
Plasmid#163148PurposeExpresses machinery necessary for selenocysteine incorporation at UAG codonsDepositorInsertsallo-tRNA UTu2D
thioredoxin
Selenophosphate synthase
Selenocysteine synthase
Cysteine desulfurase
ExpressionBacterialMutationCysteine 32 switched to Selenocysteine (UAG), Cys…PromoterEM7, araBAD, native As SelD promoter, and native …Available SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa-LSS-GFP-LDLR wt
Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorInsertLDLR (LDLR Human)
TagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN
Plasmid#11160PurposeMammalian expression vector for cloning and expressing your gene under the CAG promoterDepositorHas ServiceCloning Grade DNAInsertmodified multiple cloning sites
ExpressionMammalianAvailable SinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only
