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167,150 results

Showing: 4251 - 4300 of 167150 results

  1. FU-H2B-GFP-IRES-Puro

    Plasmid
    #69550
    Purpose
    Lentiviral, selectable fluorescent GFP tagging of nuclei.
    Depositor
    Charles Gersbach
    Insert
    Histone 2B - green fluorescent protein fusion
    Use
    Lentiviral
    Tags
    GFP fusion
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69550-plasmid-map-sequence-id-122943

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  2. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  9. psPAX2-D64V-NC-MS2

    Plasmid
    #122944
    Purpose
    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles
    Depositor
    Baisong Lu
    Article
    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531
    Insert
    NC-MCP (MS2 coat protein) fusion protein in Gag
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    D64V
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122944-plasmid-map-sequence-id-239322

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  10. T777T

    Plasmid
    #113082
    Purpose
    Enhanced RNAi vektor for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites and a lacZ coding sequence.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    RNAi
    Expression
    Bacterial
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113082-plasmid-map-sequence-id-218958

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  11. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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  19. HaloTag-A2A-adenosine receptor

    Plasmid
    #66995
    Purpose
    A2A-adenosine receptor tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    Tags
    Human Growth hormone Signal Sequence- HaloTag
    Expression
    Mammalian
    Mutation
    The signal sequence from human growth hormone was…
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66995-plasmid-map-sequence-id-115422

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  21. pLenti- V6.3 Ultra-Chili

    Plasmid
    #106173
    Purpose
    lentiviral CMV driven tdTomato-P2A-T2A, Blasticidin selectable
    Depositor
    Ewa Snaar-Jagalska
    Insert
    dTomato
    Use
    Lentiviral
    Tags
    T2A, P2A
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106173-plasmid-map-sequence-id-207442

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  23. AAV-CAG-jGCaMP8s-WPRE

    Plasmid
    #179256
    Purpose
    AAV-mediated expression of GCaMP8s under the CAG promoter
    Depositor
    Loren Looger
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8s
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    179256-plasmid-map-sequence-id-354036

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  26. pMA3790

    Plasmid
    #70110
    Purpose
    mtDNA elimination/production of rho-0 cells
    Depositor
    Mikhail Alexeyev
    Article
    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi
    Inserts
    EGFP
    hUNG1 Y147A
    Neo
    Tags
    None, human OTC mitochondrial targeting sequence,…
    Expression
    Mammalian
    Mutation
    Y147A, aa's 1-77 removed
    Promoter
    CMV, EF1a, and SV40 and native
    Available Since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    70110-plasmid-map-sequence-id-399654

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  34. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid
    #87258
    Purpose
    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.
    Depositor
    David Sibley
    Article
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    TIR1
    CAT
    Use
    Toxoplasma expression
    Tags
    3FLAG
    Mutation
    Codon optimized for Toxoplasma gondii expression
    Promoter
    TgSAG1 361 bp and TgTUB1 2717 bp
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87258-plasmid-map-sequence-id-176556

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  37. pKSE401

    Plasmid
    #62202
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    35S and AtU6-26p
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62202-plasmid-map-sequence-id-288285

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Showing: 4251 - 4300 of 167150 results