4,073 results

1. pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro

   Plasmid

   #141393

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf9c
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 8, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. Cdk6-HA

   Plasmid

   #1868

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdk6 (CDK6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D224Y

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HER2 CA (V659E)

   Plasmid

   #16259

   Depositor

   Mien-Chie Hung

   Article

   Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

   Insert

   HER2 (ERBB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Constitutively active: V659E

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. -3.6kb human IFN-gamma luc

   Plasmid

   #17599

   Depositor

   Howard Young

   Article

   Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

   Insert

   IFN gamma promoter (IFNG Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   -3.6 kb to +70 of promoter

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTOR_1 shRNA

   Plasmid

   #1855

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   mTOR (MTOR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV-Brainbow-1.0 L

   Plasmid

   #18721

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   dTomato ; mCerulean ; EYFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3.1-Nanog

   Plasmid

   #28221

   Depositor

   Linzhao Cheng

   Article

   Chou et al Cell Res. 2011 Jan 18. ():.

   Insert

   Nanog (NANOG Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMW#2

   Plasmid

   #13349

   Depositor

   Marian Walhout

   Article

   Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

   Insert

   HIS3 reporter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3 Flag Jnk2a2

   Plasmid

   #13755

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk2a2 (MAPK9 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCVpuro-Flag-cMyc T58A

    Plasmid

    #20076

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Use

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. miniSOG-C1

    Plasmid

    #54821

    Purpose

    Localization: C1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pCMV5 TBRI-His

    Plasmid

    #19161

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBigT

    Plasmid

    #21270

    Purpose

    Used for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001 . 1():4.

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FUW-tetO-hOCT4

    Plasmid

    #20726

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PERK 1: PERK.WT.9E10.pCDNA

    Plasmid

    #21814

    Depositor

    David Ron

    Article

    Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

    Insert

    PERK (Eif2ak3 Mouse)
    

    Use

    Tags

    Myc (9E10)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CMV-MyoD

    Plasmid

    #8398

    Depositor

    Andrew Lassar

    Article

    Skapek et al Mol Cell Biol 1996 Dec;16(12):7043-53.

    Insert

    MyoD (Myod1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

    Plasmid

    #12153

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Tags

    6XHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-FOXO3a TM

    Plasmid

    #1788

    Depositor

    Michael Greenberg

    Article

    Brunet et al Cell 1999 Mar 19;96(6):857-68.

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A (no longer detectably phosphor…

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Twitch-2B pcDNA3

    Plasmid

    #49531

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2B
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Cdk5-DN-HA

    Plasmid

    #1873

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk5 (CDK5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D145N, dominant negative

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAC152-dual-dCas9VP64-sgExpression

    Plasmid

    #48238

    Purpose

    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA Tag, HA-Tag, and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Thy1 promoter construct

    Plasmid

    #20736

    Depositor

    Joshua Sanes

    Article

    Feng et al Neuron. 2000 Oct . 28(1):41-51.

    Insert

    Thy1 promoter (Thy1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. IL-2 promoter luciferase

    Plasmid

    #12194

    Depositor

    Anjana Rao

    Article

    Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

    Insert

    IL-2 promoter (Il2 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 1098 pcDNA GFP FKHR

    Plasmid

    #9022

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR (FOXO1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ChR2-2A-Halo

    Plasmid

    #22047

    Purpose

    Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

    Depositor

    Edward Boyden

    Article

    Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

    Insert

    ChR2-GFP-2A-Halo-YFP
    

    Use

    Tags

    GFP and YFP

    Expression

    Mammalian

    Mutation

    Note that there is a S147R mut in YFP. This mutat…

    Promoter

    CMV

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLKO.1 puro shRNA E-cadherin

    Plasmid

    #18801

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRK5-HA GST RagB 99L

    Plasmid

    #19303

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding B (RRAGB Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    Q99L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. gRNA_GFP-T1

    Plasmid

    #41819

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HA-p73α-pcDNA3

    Plasmid

    #22102

    Depositor

    William Kaelin

    Article

    Jost et al Nature. 1997 Sep 11. 389(6647):191-4.

    Insert

    tumor protein p73 (TP73 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Sept. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-Beclin1

    Plasmid

    #21150

    Depositor

    Junying Yuan

    Article

    Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

    Insert

    PcDNA3-Beclin1 (BECN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAc-GFPC1-Sec61beta

    Plasmid

    #15108

    Depositor

    Tom Rapoport

    Insert

    Sec61beta (SEC61B Human)
    

    Use

    Tags

    Ac GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDONR223-MET

    Plasmid

    #23889

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MET (MET Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. myc mTOR kinase dead

    Plasmid

    #8482

    Depositor

    David Sabatini

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D2357E V2364I

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mTurquoise2-C1

    Plasmid

    #54842

    Purpose

    Localization: C1 Cloning Vector, Excitation: 434, Emission: 474

    Depositor

    Dorus Gadella, Michael Davidson

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBK-VQR-BE3

    Plasmid

    #85171

    Purpose

    Expresses VQR-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VQR-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMK288 (mAID-Bsr)

    Plasmid

    #72826

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Bsr
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLVET-tTR-KRAB

    Plasmid

    #11644

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hEF-1alpha, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSIN4-EF2-N2L

    Plasmid

    #21163

    Depositor

    James Thomson

    Article

    Yu et al Science. 2009 Mar 26.

    Insert

    Nanog, Lin28 (NANOG, LIN28A Human)
    

    Use

    Lentiviral; Reprogramming

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge