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We narrowed to 18,247 results for: multi

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Showing: 161 - 180 of 18247 results

  2. pCBC-DT1T2

    Plasmid
    #50590
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T1, U626t plus, U629p, T2
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50590-plasmid-map-sequence-id-210724

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  4. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Publication
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Promoter
    Human EF1a
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100547-plasmid-map-sequence-id-313417

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  9. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Publication
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71287-plasmid-map-sequence-id-352460

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  10. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Publication
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106280-plasmid-map-sequence-id-512176

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  11. GFP-P4M-SidMx2

    Plasmid
    #51472
    Purpose
    Biosensor for PtdIns4P
    Depositor
    Tamas Balla
    Publication
    Hammond et al J Cell Biol. 2014 Apr 7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    P4M-SidM(546-647)
    P4M-SidM(546-647)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51472-plasmid-map-sequence-id-457352

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  12. pdCas9

    Plasmid
    #46569
    Purpose
    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.
    Depositor
    Luciano Marraffini
    Publication
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    dcas9
    CRISPR array
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46569-plasmid-map-sequence-id-364492

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  15. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid
    #58993
    Purpose
    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter
    Depositor
    Snorri Thorgeirsson
    Publication
    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31
    Inserts
    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    EcoRV site in MCS converted to BamHI site
    Promoter
    CMV enhancer-chicken beta actin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58993-plasmid-map-sequence-id-457463

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  16. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid
    #160730
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    160730-plasmid-map-sequence-id-357931

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  18. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Publication
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60621-plasmid-map-sequence-id-208224

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  19. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Publication
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    67639-plasmid-map-sequence-id-220324

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  20. pcDNA3.1(+)/Luc2=tdT

    Plasmid
    #32904
    Purpose
    Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.
    Depositor
    Christopher Contag
    Publication
    Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Firefly Luciferase
    tandem Tomato Red Fluorescent Protein
    Tags
    tdTomato red fluorescent protein
    Expression
    Mammalian
    Promoter
    CMV-IE and n/a
    Available since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    32904-plasmid-map-sequence-id-229770

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Showing: 161 - 180 of 18247 results