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We narrowed to 9,586 results for: Coli

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Showing: 4451 - 4500 of 9586 results

  1. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225139-plasmid-map-sequence-id-460505

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  3. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    nuc-GlycoID-HA
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  8. p-spTorA-GFP-H6C

    Plasmid
    #168517
    Purpose
    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.
    Depositor
    Siegfried Musser
    Article
    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi
    Insert
    spTorA-GFP-H6C
    Tags
    6xHisC tag and spTorA (signal peptide of E. coli …
    Expression
    Bacterial
    Mutation
    The mut3 GFP variant with 5 additional mutations …
    Promoter
    araBAD
    Available Since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    168517-plasmid-map-sequence-id-346120

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  10. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid
    #169638
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    169638-plasmid-map-sequence-id-364985

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  11. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  21. pFGL1252_TetOFF(Hyg)

    Plasmid
    #118992
    Purpose
    TetOFF (promoter-less) cassette with Hygromycin selection
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    tTA+Tnos
    Hygromycin cassette
    Ttub+PtetO
    Use
    Fungal expression (in magnaporthe oryzae)
    Mutation
    Codon optimized for Magnaporthe oryzae
    Promoter
    Aspergillus nidulans TrpC promoter, Ustilago mayd…
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    118992-plasmid-map-sequence-id-232118

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  22. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid
    #52890
    Purpose
    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.
    Depositor
    Iswar Hariharan
    Article
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Inserts
    LexGAD
    Gal4 (GAL4 Budding Yeast)
    Expression
    Insect
    Available Since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    52890-plasmid-map-sequence-id-211914

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  23. pBMN YFP-ltDHFR(DD)

    Plasmid
    #47080
    Purpose
    ecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegans
    Depositor
    Thomas Wandless
    Article
    Cho et al PLoS One. 2013 Aug 22;8(8):e72393. doi:
    Insert
    YFP-ltDHFR(DD)
    Use
    Retroviral
    Tags
    HA and YFP
    Mutation
    amino acids mutations: R12H, N37D, N59D, G67S, D1…
    Promoter
    LTR
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47080-plasmid-map-sequence-id-67106

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  29. p168

    Plasmid
    #62618
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62618-plasmid-map-sequence-id-103064

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  30. pDY118A

    Plasmid
    #182958
    Purpose
    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Inserts
    Cas9
    Gam, Beta, Exo
    I-scel
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182958-plasmid-map-sequence-id-365360

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  33. Keasling-2187

    Plasmid
    #100167
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    MevT and MBIS operon
    Expression
    Bacterial
    Promoter
    lacUV5
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100167-plasmid-map-sequence-id-193500

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  38. pTS2337_Tier3(SB)-TagBFP2_Puro

    Plasmid
    #169635
    Purpose
    Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mTagBFP2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169635-plasmid-map-sequence-id-366786

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  43. pLY152

    Plasmid
    #130950
    Purpose
    dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dcas9
    tetR
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    PpspA-2G6 and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130950-plasmid-map-sequence-id-257362

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  44. pET28a-HsSENP6

    Plasmid
    #71469
    Purpose
    Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 6 (SENP6 Human)
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  47. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    codon-optimized MK
    Expression
    Bacterial
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100169-plasmid-map-sequence-id-193493

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  48. p148

    Plasmid
    #62616
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR
    Depositor
    Mattheos Koffas
    Article
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62616-plasmid-map-sequence-id-102932

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Showing: 4451 - 4500 of 9586 results