167,150 results
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Plasmid#55243PurposeLocalization: Nucleus/Histones, Excitation: 383, Emission: 448DepositorInsertH2B (H2BC11 Human)
TagsEBFP2ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
EGFR-FR
Plasmid#179263PurposeHuman EGFR C-terminally labeled with FusionRedDepositorAvailable SinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMW37: pRS426-R2Bm RNA
Plasmid#209291PurposeContains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequencesDepositorInsertR2Bm 5'UTR and 3'UTR
ExpressionBacterial and YeastPromoterGAL1 GAPDHAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJOG1368
Plasmid#167301PurposeLevel 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-FluorocytosineDepositorInsertFCY-UPP fusion gene
UseSynthetic Biology; Moclo level 0 vectorAvailable SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
DHFR-myc
Plasmid#20214DepositorAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV mGAD65(delE1) GFP WPRE SV40pA
Plasmid#177316PurposeTo produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.DepositorInsertmGAD65(delE1) promoter, GABAergic interneurons specific promoter
UseAAVAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGIG
Plasmid#11159PurposeMammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFPDepositorHas ServiceCloning Grade DNAInsertIRES-EGFP
ExpressionMammalianAvailable SinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
UseCRISPR and LentiviralExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable SinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE57
Plasmid#90402PurposeType I Interferon - ISGF3G (STAT1, STAT2, IRF9) gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterISREAvailable SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CASR-Tango
Plasmid#66235PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA_PDS
Plasmid#46966PurposeExpresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoterDepositorInsertAtU6p::sgRNA_PDS
UseCRISPR; Plant expressionAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rnaā¦Available SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT_htr2c(c)-GFP
Plasmid#79677Purposeserotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFPDepositorInsertserotonin receptor 2C (HTR2C Human)
TagsEGFP in FrameExpressionMammalianMutationsplice site of exon Vb in consensusPromoterCMVAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b-His tagged nuclear Pif1
Plasmid#65047Purposeexpression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tagDepositorInsertYeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
TagshistagExpressionBacterialPromoterT7 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_Zeo
Plasmid#118596PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
ExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer10b-EGFP-KRAS G12V
Plasmid#164925PurposeExpresses EGFP cDNA and KRAS G12V cDNA in mammalian cellsDepositorInsertsUseLentiviralMutationGlycine 12 changed to valine (G->T 3156)PromoterUbc Promoter and Ubc promoterAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP713-N1
Plasmid#197230PurposeExpresses the fusion protein of H2B-miRFP713 in mammalian cellsDepositorInsertH2B-miRFP713
ExpressionMammalianAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIPH4
Plasmid#117685PurposeHansenula polymorpha expression plasmidDepositorInsertAlcohol Oxidase promoter
ExpressionYeastPromoterAlcohol OxidaseAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBY1_FnCpf1cg
Plasmid#104622PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn CPf1DepositorInsertFnCpf1
UseCRISPRExpressionBacterialMutationcodon-optimized C.glutamicumPromoterhsp60Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-M2L
Plasmid#20926Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28DepositorArticleAvailable SinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits only -
pICSL90003
Plasmid#68262PurposeLevel 0 Golden Gate Part: Promoter U6 (Triticum aestivum)DepositorInsertPromoter: U6 (Triticum aestivum)
UseSynthetic BiologyAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4
Plasmid#226372PurposeProduction of recombinant mEGFP-D4, a fluorescent probe binding cholesterolDepositorInsertD4 (pfoA Clostridium perfringens)
TagsHis6 and monomeric EGFPExpressionBacterialPromoterT7Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF2857
Plasmid#143642PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tet2
Plasmid#60939PurposeExpresses Tet2 in mammalian cellsDepositorAvailable SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PINK1 gRNA (BRDN0001145357)
Plasmid#78036Purpose3rd generation lentiviral gRNA plasmid targeting human PINK1DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CD14
Plasmid#13645DepositorInsertCD14 (CD14 Human)
ExpressionMammalianAvailable SinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
mCh-alpha-tubulin
Plasmid#49149Purposeexpression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.DepositorAvailable SinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LaminA
Plasmid#231894PurposeExpression of HaloTag-tagged LaminA to visualize the nuclear laminaDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSYC-437
Plasmid#163829PurposeFoxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.DepositorInsertsFoxj1a Promoter
GCaMP6s
UseMultisite gateway destination vectorAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT13-splitC
Plasmid#122596Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
ExpressionBacterialAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
Plasmid#187334PurposedCas9 effector fusion for manipulating transcriptionDepositorInsertdCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast
UseCRISPR and LentiviralTags3XFLAGExpressionMammalianPromoterpEF1aAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV-sct-pp65-b2m-HLA-A0201
Plasmid#196511PurposeExpress single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)DepositorInsertsingle chain of a pp65 peptide, b2m and HLA A0201
UseLentiviralTagsNAExpressionMammalianMutationY84CPromoterCMVAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi:loxP-EGFP-loxP
Plasmid#27322DepositorInsertubiquitin:loxP-EGFP-loxP
UseMultisite gateway 5' entry vectorAvailable SinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IDO1-p2a-eGFP
Plasmid#187026PurposeExpresses IDO1 and GFP (seperately) in mammalian cellsDepositorAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Lamin-A
Plasmid#206027PurposeExpresses GFP-tagged Lamin A in mammalian cellsDepositorAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1-neo-TET
Plasmid#51284Purposeexpresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassetteDepositorInsertL1RE1 (L1RE1 Human)
TagsneoTET cassette with a tetrahymena self-splicing ā¦ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-AVP
Plasmid#184600PurposeFor expression of a fluorescent sensor for vasopressin in mammalian cellsDepositorInsertMTRIA-AVP
ExpressionMammalianPromoterCAG promoterAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-RBCS-tagRFP
Plasmid#213868PurposeBinary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertRIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
ExpressionBacterial and PlantAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-mCherry
Plasmid#213862PurposeBinary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused mCherry-UnaG
ExpressionBacterial and PlantAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
miniSOG-H2B-C-10
Plasmid#57769PurposeLocalization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertH2B (H2BC11 Human)
TagsminiSOGExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable SinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDNA3 Flag p38 gamma
Plasmid#20353DepositorAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc-LATS1
Plasmid#66851PurposeExpress Myc-tagged LATS1 in mammalian cellsDepositorInsertLATS1 (LATS1 Human)
TagsMycExpressionMammalianMutationP44L mutation in LATS1 compared to GenBank refereā¦PromoterCMVAvailable SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR
Plasmid#162335PurposeLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2_mSTING_scrambled_gRNA_1
Plasmid#196627PurposeScrambled control 1 for STING knock-out in murine cells.DepositorInsertmSTING scrambled gRNA 1
UseCRISPR and LentiviralMutationWTAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHG6
Plasmid#188254PurposeIntegrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT138b-proD
Plasmid#138516PurposeP3eDepositorInsertT7-RNAP
UseSynthetic BiologyAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES-mtGFP
Plasmid#45053DepositorInsertmtGFP
ExpressionYeastPromoterGALAvailable SinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf1-16
Plasmid#198271PurposeUnmodified pPVC; contains WT PVC structural/accessory genesDepositorInsertPVCpnf1-16
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G
Plasmid#204188PurposeDoxycycline inducible lentiviral vector for human NOTUM overexpressionDepositorAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIS.MTRIA-OT
Plasmid#184596PurposeFor expression of a fluorescent sensor for oxytocin in mammalian cellsDepositorInsertMTRIA-OT
ExpressionMammalianPromoterCAG promoterAvailable SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only