We narrowed to 13,195 results for: 109

1. NAR4-CD4d3+4-bio

   Plasmid

   #73118

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding NAR4 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   NAR4 (ART4 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mEGFP-N1-NF2

   Plasmid

   #166473

   Purpose

   Expresses fusion of mEGFP and NF2

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, NF2 (NF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. G-GECO1.2-Orai1Y80E

   Plasmid

   #73565

   Purpose

   Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1.2-Orai1Y80E (ORAI1 Human, Synthetic)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   changed tyrosine 80 of Orai1 to glutamate

   Promoter

   CMV Immediate Early

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLEX307-HCoV-NL63-3CL-C144A

   Plasmid

   #160287

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C144A

   Promoter

   EF1a

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMCP-MU-Luc (MCP-1 mut promoter in pGL3-basic)

   Plasmid

   #40325

   Depositor

   Alexander Dent

   Article

   Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

   Insert

   MCP-1 promoter (mutated) (Ccl2 Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   contains MCP-1 promoter region through the nucleo…

   Promoter

   MCP-1

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX307-SARS-CoV-3CL-C145A

   Plasmid

   #160281

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NLS-tdPCP-CIBN

   Plasmid

   #183937

   Purpose

   PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdPCP-CIBN (CIB1 Mustard Weed, Synthetic)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p4949 pLPCX-His-Xpress NLS hBrd4 CTD

   Plasmid

   #14459

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 CTD (BRD4 Human)
   

   Use

   Retroviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   amino acids 1047-1362 of Brd4 (the CTD). Function…

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

9. Clover-Mito-7

   Plasmid

   #56529

   Purpose

   Localization: Mitochondria, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Mito (COX8A Human)
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUHD-HA-AML1-ETO

    Plasmid

    #12430

    Depositor

    Dong-Er Zhang

    Article

    Rhoades et al Blood. 2000 Sep 15. 96(6):2108-15.

    Insert

    AML1-ETO (RUNX1, RUNX1T1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    BstXI-SacII fragment in pUHD-AML1-ETO replaced wi…

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pLVX neo ACTN1-GFP, Y246E

    Plasmid

    #138294

    Purpose

    Mammalian Expression, Lentiviral. Expression of human alpha-actinin-1-GFP, Y246E fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    ACTN1 (ACTN1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Y246E

    Promoter

    CMV

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. sgMAPKAP1_1

    Plasmid

    #124912

    Purpose

    Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Tet-pLKO-puro shPOLE1_1

    Plasmid

    #160810

    Purpose

    Express Dox repressible shPOLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX307-MERS-CoV-3CL-C148A

    Plasmid

    #160283

    Purpose

    Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C148A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-GP-2-CBX1 Chromo

    Plasmid

    #59697

    Purpose

    Contains histone modification interacting domain (CBX1 Chromo)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    CBX1 Chromo (CBX1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST15.1-hRPN13 (285-386)

    Plasmid

    #61936

    Purpose

    Expression of hRPN13 (285-386) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hRPN13 (285-386) (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Construct starts at residue 285, stop codon intro…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pLEX307-IBV-3CL-C143A

    Plasmid

    #160289

    Purpose

    Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    C143A

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mTFP1-MAPTau-N-10

    Plasmid

    #55498

    Purpose

    Localization: Microtubules, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    MAPTau (MAPT Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pPPC010.AAV

    Plasmid

    #171175

    Purpose

    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    BBa_J23119 and Sp.pCas9, BBa_J23107

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP Kind2 S181E

    Plasmid

    #105312

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181E

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEGFP Kind2 S181A

    Plasmid

    #105307

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5 GST MetaP2

    Plasmid

    #112750

    Purpose

    express HA-GST-FRB-MetaP2

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    HA-GST-FRB-MetaP2 (METAP2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. sgMAPKAP1_2

    Plasmid

    #124911

    Purpose

    Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. sgMAPKAP1_3

    Plasmid

    #124913

    Purpose

    Targeting human MAPKAP1 (SIN1) gene by CRISPR-Cas9

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MAPKAP1 (MAPKAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ORAI1-YFP T184M

    Plasmid

    #114181

    Purpose

    ORAI1 channel with C-terminal YFP, carrying the mutation T184M in the protein 3rd transmembrane domain, responsible for the channel overactivity and associated with tubular aggregate myopathy (TAM).

    Depositor

    Nicolas Demaurex

    Article

    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

    Insert

    ORAI1-YFP T184M (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    T184M

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-RNF138-delta 86-105

    Plasmid

    #78922

    Purpose

    Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 86-105

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pEGFP-RNF138-delta 158-180

    Plasmid

    #78923

    Purpose

    Mammalian expression of RNF138 with delta 158-180 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 159-180

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pEGFP-RNF138-delta 189-215

    Plasmid

    #78924

    Purpose

    Mammalian expression of RNF138 with delta 189-215 and an EGFP fusion

    Depositor

    Michael Hendzel

    Article

    Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

    Insert

    RNF138 (RNF138 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta 189-215

    Promoter

    CMV

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pCGT-HCF-1-C600

    Plasmid

    #99364

    Purpose

    C-term of HCFC1 protein

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 2000 Sep;20(18):6721-30.

    Insert

    HCF-1 C-term 1436-2035 (HCFC1 Human)
    

    Use

    Tags

    2x tandem T7 tag and C-myc

    Expression

    Mammalian

    Mutation

    Deletion of HCFC1 cDNA

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMTTH+mEGFP-LANA-H1.4CTD

    Plasmid

    #157802

    Purpose

    Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-LANA-H1.4CTD (H1-4 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mCardinal-Alpha-Actinin-19

    Plasmid

    #56152

    Purpose

    Localization: Focal Adhesions / Actin, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

33. pFRT-TODestFLAGHA_RBPMS

    Plasmid

    #59388

    Purpose

    Destination vector with RBPMS for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CRAF_S29A_pcDNA3.1

    Plasmid

    #86506

    Purpose

    Expresses CRAF_S29A in mammalian cells [Edit]

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    CRAF (RAF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S29A

    Promoter

    CMV

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGL4.23 T230A 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86017

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed threonine 230 to alanine

    Promoter

    CMV IE

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86018

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed aspartic acid 637 to glycine

    Promoter

    CMV IE

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pADSL-2XStrep

    Plasmid

    #125684

    Purpose

    expresses human ADSL protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    ADSL (ADSL Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD

    Plasmid

    #14442

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 CTD (BRD4 Human)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    amino acids 1047-1362 of Brd4 (the CTD). Function…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

39. Opto-GPR75

    Plasmid

    #106032

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR75; Opto-GPR75; C10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR75 (GPR75 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. B52 + TIMELESS sgSTOP

    Plasmid

    #100713

    Purpose

    B52 plasmid expressing TIMELESS sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting TIMELESS (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. B52 + PARP4 sgSTOP

    Plasmid

    #100711

    Purpose

    B52 plasmid expressing PARP4 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting PARP4 (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Use

    Tags

    3x Flag and myc

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

43. pMCSF-R(dAB)-luc

    Plasmid

    #12423

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    M-CSF receptor promoter fragment (CSF1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    No AML1 and C/EBP binding sites (delete bp -86 to…

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

44. pGEX-6P-1 p37 SHPmut

    Plasmid

    #113503

    Purpose

    Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    F215A/E218A/QKL220-222AAA

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pfast bac Rbx-HA

    Plasmid

    #51893

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Rbx1 (RBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

46. pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128337

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna2 (Chrna2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. B52 + PIK3R1 sgSTOP

    Plasmid

    #100714

    Purpose

    B52 plasmid expressing PI3KR1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting PIK3R1 (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGEX-6P-1 p37 deltaUBX

    Plasmid

    #113504

    Purpose

    Bacterial expression of GST-tagged p37 lacking the UBX domain

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 251-331 (stop codon behind AA250)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGL4.23 A82V/T230A 2014 EBOV + Mucin-Like Domain

    Plasmid

    #86019

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed alanine 82 to valine and threonine 230 to…

    Promoter

    CMV IE

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. hnRNPA2_LC_P298L_S285C

    Plasmid

    #104469

    Purpose

    express His tagged P298L S285C hnRNPA2 LC

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    P298L S285C

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge