8,883 results

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pCAG-SpCas9-GFP-U6-gRNA

Plasmid

#79144

Purpose

All-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSC

Depositor

Jizhong Zou

Article

Stoehr et al JCI Insight. 2019 May 21;5. pii: 128496.

Insert

SpCas9

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pInducer10-mir-RUP-PheS

Plasmid

#44011

Purpose

Lentiviral shRNA vector (pGIPz based) for inducible knockdown

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Promoter

TRE2

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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shp53 pLKO.1 puro

Plasmid

#19119

Purpose

3rd gen lentiviral vector for knocking down p53 gene expression

Depositor

Bob Weinberg

Article

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against tumor protein p53 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 9, 2008

Availability

Academic Institutions and Nonprofits only

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mAzurite-N1

Plasmid

#54617

Purpose

Localization: N1 Cloning Vector, Excitation: 384, Emission: 450

Depositor

Patrick Daugherty, Michael Davidson

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Type

Empty backbone

Tags

mAzurite

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCPRDEnv

Plasmid

#1732

Purpose

This is the FIV PPR based second generation packaging vector, part of the FELIX vector system from Nolan lab

Depositor

Garry Nolan

Insert

Gag-Pol and Rev

Expression

Mammalian

Available Since

Oct. 24, 2005

Availability

Academic Institutions and Nonprofits only

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pmAmetrine-DEVD-tdTomato

Plasmid

#18879

Purpose

Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato

Depositor

Robert Campbell

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Insert

mAmetrine-DEVD-tdTomato (CASP3 Human, lab constructed)

Expression

Mammalian

Mutation

mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pET11phiC31poly(A)

Plasmid

#18942

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.GFP

Plasmid

#57818

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

EFS and hU6

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pQE30-His-PheRS

Plasmid

#124116

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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TOP-GFP

Plasmid

#35489

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xTOP-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

7xTCF/LEF

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pmCherry Vinculin

Plasmid

#50527

Depositor

Kenneth Yamada

Insert

Vinculin (VCL Chicken)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAC-GAL4

Plasmid

#24344

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

GAL4

Expression

Insect

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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mCherry-Vimentin-7

Plasmid

#55156

Purpose

Localization: Intermediate Filaments, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Vimentin (VIM Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pLV-tetO-Eomes

Plasmid

#70271

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Eomes

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Eomes (Eomes Mouse)

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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Tom20-V5-FKBP-AP_pLX304

Plasmid

#120914

Purpose

Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

Tom20-V5-FKBP-AP

Use

Lentiviral

Expression

Mammalian

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-Traptavidin

Plasmid

#24753

Depositor

Mark Howarth

Article

Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

Insert

Traptavidin

Tags

His-6

Expression

Bacterial

Mutation

Streptavidin with 2 amino acid mutations: S52G an…

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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Str-Ii_VSVG-SBP-mCherry

Plasmid

#65301

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3β-FLAG-CBP-HA

Plasmid

#32908

Depositor

Tso-Pang Yao

Article

Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

Insert

CBP (Crebbp Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf1 ActQ71L

Plasmid

#10832

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 Q71L (ARF1 Human)

Tags

HA

Expression

Mammalian

Mutation

Q71L. Defective in GTP hydrolysis. Constitutive…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pT7-CalfluxVTN

Plasmid

#83926

Purpose

Bioluminescent Reporter of Intracellular Calcium

Depositor

Carl Johnson

Article

Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

Insert

CalfluxVTN

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Neo(+) hSUMF1

Plasmid

#24386

Depositor

Carolyn Bertozzi

Article

Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

Insert

hSUMF1 (SUMF1 Human)

Expression

Mammalian

Available Since

April 2, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
L304-EGFP-Tubulin-WT

Plasmid

#64060

Purpose

EGFP fused at the N-terminus of tubulin; lentiviral construct

Depositor

Weiping Han

Article

Yang et al Biochem J. 2013 Feb 1;449(3):605-12. doi

Insert

tubulin

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSV Sport RXR alpha

Plasmid

#8882

Depositor

Bruce Spiegelman

Article

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

RXR alpha (RXRA Human)

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pF141 pAcGFP1 SOD1WT

Plasmid

#26402

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL4UAS-Luciferase reporter

Plasmid

#64125

Purpose

Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

GAL4UAS

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GAL4UAS

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-FlpO

Plasmid

#89574

Purpose

Expresses FlpO under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28 MBP_PrA/G-H6

Plasmid

#176077

Purpose

Express His6 tagged MBP_PrA/G in Escherichia coli

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

MBP PrA/G-H6

Tags

His tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLB

Plasmid

#11619

Purpose

pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

Depositor

Stephan Kissler

Article

Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

Plasmid

#149276

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK-Myc-Smurf2

Plasmid

#13678

Depositor

Ying Zhang

Article

Zhang et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

Smurf2 (SMURF2 Human)

Tags

Myc

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
paavCAG-post-mGRASP-2A-dTomato

Plasmid

#34912

Purpose

post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Has Service

AAV5

Insert

postsynaptic mGRASP

Use

AAV

Promoter

CAG

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HIVGKO

Plasmid

#112234

Purpose

HIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1a

Depositor

Eric Verdin

Article

Battivelli et al Elife. 2018 May 1;7. pii: 34655. doi: 10

Insert

csGFP-EF1a-mKO2

Use

Lentiviral

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAP01

Plasmid

#59981

Purpose

The function of this material is to facilitate the capture and expression of bacterial gene clusters.

Depositor

Bradley Moore

Article

Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

Type

Empty backbone

Use

Synthetic Biology; Transformation-associated reco…

Tags

no

Expression

Bacterial and Yeast

Promoter

no

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-FOXO3a WT

Plasmid

#8360

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

FOXO3a (FOXO3 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFPC1-huNFATc1EE-WT

Plasmid

#24219

Depositor

Jerry Crabtree

Article

Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

Insert

NFATc1 (NFATC1 Human)

Tags

EE and GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET GFP LIC cloning vector (2GFP-T)

Plasmid

#29716

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, SiriusGFP, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pOSIP-KO (KanR, 186)

Plasmid

#45985

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/Luc2=tdT

Plasmid

#32904

Purpose

Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

Depositor

Christopher Contag

Article

Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

Inserts

Firefly Luciferase
tandem Tomato Red Fluorescent Protein

Tags

tdTomato red fluorescent protein

Expression

Mammalian

Promoter

CMV-IE and n/a

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet-1∆HisTag_hNMT_Nef

Plasmid

#66078

Purpose

Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

Depositor

Dieter Willbold

Article

Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

Inserts

NMT (NMT1 Human)
Nef

Expression

Bacterial

Mutation

Deletion of first 80 amino acids

Promoter

T7-lacO

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-Sec61 beta

Plasmid

#49155

Purpose

expression of Sec61 beta fused to mCherry, used as a general ER marker

Depositor

Gia Voeltz

Article

Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

Insert

Sec61 beta (SEC61B Human)

Tags

mCherry*

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge

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8,883 results