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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,032 results for: ENA

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Showing: 6001 - 6050 of 13032 results

  7. pENTR_L1-YFP-Turbo-L2

    Plasmid
    #127350
    Purpose
    gateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteins
    Depositor
    Dominique Bergmann
    Article
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Insert
    TurboID (BirA mutant)
    Use
    Gateway entry vector
    Tags
    GS linker, V5, and YFP
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127350-plasmid-map-sequence-id-246303

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  10. pCMV-IRES-Renilla Luciferase-IRES-Gateway-Firefly Luciferase (pIRIGF)

    Plasmid
    #101139
    Purpose
    Luciferase reporter plasmid. Note that both renilla luciferase and ORF-firefly luciferase are encoded by a single mRNA and both are translated independently via internal ribosomal binding sites.
    Depositor
    William Kaelin
    Article
    Lu et al Science. 2014 Jan 17;343(6168):305-9. do
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    101139-plasmid-map-sequence-id-194900

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  18. pBHM2403

    Plasmid
    #171687
    Purpose
    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    CnoCAS9
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    TEF1
    Available Since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171687-plasmid-map-sequence-id-340174

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  26. pHeACT-mEGFP-NLS

    Plasmid
    #205003
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
    Use
    Tardigrade expression
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205003-plasmid-map-sequence-id-405176

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  28. pJH2685

    Plasmid
    #191372
    Purpose
    Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP
    Depositor
    Mei Zhen
    Article
    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e
    Insert
    Tetanus Toxin Light Chain
    Tags
    wCherry
    Expression
    Worm
    Promoter
    Punc-4
    Available Since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    191372-plasmid-map-sequence-id-377546

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  34. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Expression
    Bacterial
    Available Since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  42. pDIRECT_23C

    Plasmid
    #91140
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91140-plasmid-map-sequence-id-182268

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  43. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

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Showing: 6001 - 6050 of 13032 results