166,365 results

1. pET30-2-GAPDH

   Plasmid

   #83910

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. V. natriegens NC7

   Bacterial Strain

   #215356

   Purpose

   Vibrio natriegens with genomically integrated natural competence regulator, TfoX. For zero-capital molecular biology applications.

   Depositor

   Buz Barstow

   Article

   Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

   Bacterial Resistance

   None

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

3. pDONR223_EGFR_p.769_770insASV

   Plasmid

   #82880

   Purpose

   Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, Matthew Meyerson

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   ASV insertion between AA 769 and 770

   Promoter

   None

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ108 - pENTR221 - [cbr-unc-119(+)]

   Plasmid

   #200367

   Purpose

   cbr-unc-119 rescue gene for use as co-injection marker.

   Depositor

   Christian Froekjaer-Jensen

   Article

   AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

   Insert

   [cbr-unc-119(+)]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW

   Plasmid

   #14882

   Depositor

   David Baltimore

   Article

   Lois et al Science. 2002 Feb 1. 295(5556):868-72.

   Insert

   flap-Ub promoter-WRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAZ-SK

   Plasmid

   #73962

   Purpose

   self-killing gRNA plasmid pMAZ-SK

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of AA 1 to 45

   Promoter

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EF1α1.1-GFP

   Plasmid

   #122100

   Purpose

   AAV-mediated expression of GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV2

   Insert

   GFP
   

   Use

   AAV

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available Since

   May 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-pgk-Cre (AAV Retrograde)

    Viral Prep

    #24593-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from AAV-pgk-Cre (#24593). In addition to the viral particles, you will also receive purified AAV-pgk-Cre plasmid DNA. Cre expression under the PGK promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.

    Depositor

    Patrick Aebischer

    Promoter

    pgk

    Tags

    None

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pTetOFF SNCA-A53T

    Plasmid

    #215372

    Purpose

    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Promoter

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-wtVHL

    Plasmid

    #13390

    Depositor

    Lucy Anderson

    Article

    Shiao et al Cancer Res. 2000 Jun 1. 60(11):2816-9.

    Insert

    von Hippel-Lindau (Vhl Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-VSVP

    Plasmid

    #64088

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV phosphoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLX1.8NFATGFPpd2HS4

    Plasmid

    #169096

    Purpose

    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

    Depositor

    Geoffrey Owens

    Inserts

    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFAT enhancer + synthetic TATA box

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG241 FKBP-CFAST10

    Plasmid

    #130814

    Purpose

    Expresses FKBP-CFAST10 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FKBP-CFAST10
    

    Use

    Tags

    Myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-EGFP-P2A-iCre (JDW 921)

    Plasmid

    #224519

    Purpose

    A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    EGFP-P2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBABE-neo largeTcDNA

    Plasmid

    #1780

    Purpose

    Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. CDK12 gRNA (BRDN0001147156)

    Plasmid

    #75915

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK12

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK12 (CDK12 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBluescriptKS(-)-FRB

    Plasmid

    #104475

    Purpose

    Plasmid carrying FRB with C-terminal HA tag.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    FRB (MTOR Human)
    

    Use

    Phagemid

    Tags

    HA tag

    Expression

    Mutation

    includes only the FRB domain

    Promoter

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. CDK12 gRNA (BRDN0001147294)

    Plasmid

    #75916

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK12

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK12 (CDK12 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. spNW50

    Plasmid

    #173486

    Purpose

    Expresses spNW50 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW50
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCold I-Hero45

    Plasmid

    #187931

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero45

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero45 (SERBP1 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-6xHis-(pos36)GFP

    Plasmid

    #62937

    Purpose

    Expression of 6xHis-(+36)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (pos36)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PHR-GFP-FUSN-VP16

    Plasmid

    #183933

    Purpose

    Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-FUSN-VP16 (CRY2, UL48 Mustard Weed, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

    Pooled Library

    #136479

    Purpose

    Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

    Depositor

    Jonathan Weissman

    Article

    Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

26. pGWB452

    Plasmid

    #74834

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP-FKBP(M)x4

    Plasmid

    #85003

    Purpose

    Expresses EGFP-FKBP(M)x4 in yeast

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    EGFP-FKBP(M)x4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-CAG-UnaG-P2A-FusionRed

    Plasmid

    #191109

    Purpose

    Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    UnaG
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWR76

    Plasmid

    #202776

    Purpose

    episomal fluorescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBlu-CGT-1

    Plasmid

    #170158

    Purpose

    Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

    Depositor

    Sarah MacDonald

    Insert

    Cyclomaltodextrin Glucanotransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TFORF3536

    Plasmid

    #145012

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXD4 (HOXD4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMBP-HRV3C-AcrIF9

    Plasmid

    #141442

    Purpose

    Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

    Depositor

    Blake Wiedenheft

    Article

    Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

    Insert

    AcrIF9
    

    Use

    Tags

    HRV3C cleavage site and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. BB2_AB

    Plasmid

    #98534

    Purpose

    empty BB2 for transcription unit cloning

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDONR223_EGFR_p.G719D

    Plasmid

    #81708

    Purpose

    Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    G719D

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Human Brunello kinome pooled library, guides 1-8 in lentiGuide-Puro backbone

    Pooled Library

    #1000000082

    Purpose

    CRISPR gRNA knockout pooled library for targeting the human kinome

    Depositor

    David Root, John Doench

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pAAV.CAG.iAChSnFR (AAV9)

    Viral Prep

    #137955-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.CAG.iAChSnFR (#137955). In addition to the viral particles, you will also receive purified pAAV.CAG.iAChSnFR plasmid DNA. CAG-driven iAChSnFR acetylcholine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Promoter

    CAG

    Tags

    None

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

37. pAAV-CAG-dLight1.3b (AAV9)

    Viral Prep

    #125560-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    CAG

    Tags

    None

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

38. plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

    Plasmid

    #209728

    Purpose

    Expresses cytosolic high affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95K.S29W.miRFP670nano3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Rtn4a-GFP

    Plasmid

    #61807

    Purpose

    Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

    Depositor

    Gia Voeltz

    Article

    Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Rtn4a Stop Codon removed

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

    Plasmid

    #154473

    Purpose

    Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Promoter

    SFFV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge