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179,538 results

Showing: 7001 - 7050 of 179538 results

  15. pAAV2/5

    Plasmid
    #232922
    Purpose
    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Rep2/Cap5
    Use
    AAV
    Expression
    Mammalian
    Available since
    Sept. 17, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    232922-plasmid-map-sequence-id-458562

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  20. pAAV-nEF Con/Fon DREADD Gq-mCherry

    Plasmid
    #183532
    Purpose
    INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp
    Depositor
    Karl Deisseroth
    Insert
    DREADD Gq-mCherry
    Use
    AAV
    Mutation
    None
    Promoter
    nEF
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    183532-plasmid-map-sequence-id-362037

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  21. pHN924

    Plasmid
    #178885
    Purpose
    Expression of GST-His8-HRV3C
    Depositor
    Kazuhiro Abe
    Insert
    human rhinovirus 3C protease
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    178885-plasmid-map-sequence-id-354971

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  23. pAAV-hsyn-GRAB_rDA1h (AAV9)

    Viral prep
    #140557-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1h (#140557). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1h plasmid DNA. Synapsin-driven expression of red fluorescent, high affinity dopamine sensor GRAB_rDA1h. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Publication
    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.
    Promoter
    Syn
    Available since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only

  24. pAAV-Ef1a-Con/Foff 2.0-sRGECO (AAV8)

    Viral prep
    #137127-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-sRGECO (#137127). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-sRGECO plasmid DNA. EF1a-driven, Cre-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Flp). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  25. pAAV-Ef1a-Coff/Fon-mCherry (AAV8)

    Viral prep
    #137134-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-mCherry (#137134). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-mCherry plasmid DNA. EF1a-driven, Flp-dependent expression of mCherry (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only

  26. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

    Plasmid
    #162380
    Purpose
    AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Has service
    AAV1, AAV9, and AAV Retrograde
    Insert
    jGCaMP8s
    Use
    AAV and Cre/Lox
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    S26M F286Y Q315H
    Promoter
    CAG
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    162380-plasmid-map-sequence-id-318379

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  27. pLenti-PalmGRET

    Plasmid
    #158221
    Purpose
    BRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.
    Depositor
    Charles P. Lai
    Publication
    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014
    Insert
    PalmGFP-Nluc
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    158221-plasmid-map-sequence-id-309022

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  29. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

    Plasmid
    #149276
    Purpose
    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs
    Depositor
    Savithramma Dinesh-Kumar
    Publication
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    Use
    T-dna vector
    Mutation
    T2318C (DNA) in PEBV coat protein sequence
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    149276-plasmid-map-sequence-id-300510

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  31. pDEST-CMV-polysite

    Plasmid
    #122843
    Purpose
    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Type
    Empty backbone
    Use
    Gateway Cloning
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    122843-plasmid-map-sequence-id-244374

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  36. pKC-4.04

    Plasmid
    #112085
    Purpose
    Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin
    Depositor
    James Inglese
    Publication
    Baird et al eLife. 2018 Mar 12;7. pii: 33178. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3XFLAG firefly luciferase-beta-globin (HBB Mouse, Photinus pyralis, Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    112085-plasmid-map-sequence-id-214511

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  37. pAAV.CamKII(1.3).eYFP.WPRE.hGH (AAV1)

    Viral prep
    #105622-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CamKII(1.3).eYFP.WPRE.hGH (#105622). In addition to the viral particles, you will also receive purified pAAV.CamKII(1.3).eYFP.WPRE.hGH plasmid DNA. Expression of EYFP from CaMKII(1.3) promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII(1.3)
    Tags
    EYFP
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  38. pEGFP-N1-IL6

    Plasmid
    #111933
    Purpose
    Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells
    Depositor
    Geert van den Bogaart
    Publication
    Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Interleukin 6 (IL6 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    CMV
    Available since
    July 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    111933-plasmid-map-sequence-id-213785

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  41. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40

    Plasmid
    #105545
    Purpose
    AAV expression of EGFP-Cre from CMV promoter
    Depositor
    James M. Wilson
    Has service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    EGFP-Cre
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    HI
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52 Articles
    105545-plasmid-map-sequence-id-202462

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  43. PB-sgRNA(MS2)

    Plasmid
    #102560
    Purpose
    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.
    Depositor
    Ying Liu
    Publication
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    102560-plasmid-map-sequence-id-198595

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  44. pIVTRup

    Plasmid
    #101362
    Purpose
    Highly efficient in vitro transcription. Empty backbone.
    Depositor
    Ángel Raya
    Insert
    5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
    Use
    For cloning a gene for in vitro transcription pur…
    Promoter
    T7
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    101362-plasmid-map-sequence-id-352564

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  45. eSpCas9(1.1)-T2A-Puro

    Plasmid
    #101039
    Purpose
    Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.
    Depositor
    Andrea Németh
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    3xFLAG and T2A-Puro
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    101039-plasmid-map-sequence-id-190363

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  46. Tau (P301L)-VC

    Plasmid
    #87633
    Purpose
    expresses hTau fused to the C-terminal part of Venus
    Depositor
    Tiago Outeiro
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPT (MAPT Human)
    Tags
    N-terminal part of Venus
    Expression
    Mammalian
    Mutation
    Mutation P301L
    Promoter
    CMV
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    87633-plasmid-map-sequence-id-196952

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Showing: 7001 - 7050 of 179538 results