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Plasmid#143412PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZFAT (ZFAT Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWUR703
Plasmid#53082Purposeinducible TtAgoDM (D478A,D546A) expressionDepositorInsertTtAgo
UseTagsStrep-II tagExpressionBacterialMutationD478A D546APromoterT7Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV/D374Y-hPCSK9
Plasmid#58379PurposeExpresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosisDepositorInsertproprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
UseAAVTagsExpressionMammalianMutationD374YPromoterHCRApoE/hAATAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMS2M-PH-gagpol-D64V
Plasmid#166031PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertMS2-Gag-Pol
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-GABARAPL2 WT
Plasmid#123103PurposeExpresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.DepositorInsertGamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cre-IRES2-GFP
Plasmid#26646PurposeMammalian coexpression of nls-Cre and EGFP from the CAG promoterDepositorInsertCre
UseCre/LoxTagsGFP and IRES2ExpressionMammalianMutationPromoterCAGAvailable sinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKI_ΩBBMVP16&SRDXWUSm1
Plasmid#220349PurposeExpresses BBM-VP16 and SRDX-WUSm1 in plant cellsDepositorUseTagsSuperman Repression Domain X (SRDX) and VP16 tran…ExpressionPlantMutationtwo amino acid substitution in WUS-box: L255A L25…PromoterCaMV 35S and RPS5AAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CA-H1047R
Plasmid#116500PurposeLentiviral expression of PIK3CA H1047RDepositorInsertPIK3CA (PIK3CA Human)
UseLentiviralTagsExpressionMutationH1047RPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-ER WT
Plasmid#49498PurposeExpresses HA-tagged ER wild-type in mammalian cellsDepositorInsertESR1 (ESR1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-mCh
Plasmid#187841PurposeConstitutive mCherry-expression vector for Fusobacterium nucleatumDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-218-5p
Plasmid#103357PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-218-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN-AIDA-Tyr1
Plasmid#192831PurposeIPTG inducible AIDA-Tyrosinase expressionDepositorInsertAIDA-Tyr1
UseTagsExpressionBacterialMutationPromoterpL-lacAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
NFAT luciferase reporter
Plasmid#10959DepositorInsertIL-2 promoter (IL2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-KDM2B-P2A-mCherry
Plasmid#203852PurposeTet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorInsertKDM2B (KDM2B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Lysosomes-20
Plasmid#55073PurposeLocalization: Lysosome Membrane, Excitation: 587, Emission: 610DepositorInsertLAMP1
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Flex-FlpO
Plasmid#191202PurposeCre dependent FlpO expressionDepositorInsertFlpO
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF150 pSOD1G93AAcGFP1
Plasmid#26411Purposecontains G94A mutation in pSOD1DepositorInsertSOD1 (SOD1 Human)
UseTagsGFPExpressionMammalianMutationG94APromoterAvailable sinceNov. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CoA-Snifit(V97T)
Plasmid#197944PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-V97T, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_CoA-Snifit(G41)
Plasmid#197942PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)
Plasmid#197943PurposeExpression of CoA-Snifit in mammalian cellsDepositorInsertHalo-GFP-ecPanK
UseTagsExpressionMammalianMutationPanK=PanK-G41S, D127A,L277W,F252YPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-PGK-Cit-tvmvs-DHFR
Plasmid#116042PurposeReporter plasmid for measuring protease activityDepositorInsertCit-tvmvs-DHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX304-IRES-GFP
Plasmid#176288PurposepLX304 backbone with an IRES-GFP cassette subcloned into the NheI-AgeI sitesDepositorTypeEmpty backboneUseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLIK Zeo p53DD
Plasmid#136534PurposeLentiviral expression vector for an inducible p53DDDepositorInsertp53DD (Trp53 Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationThis construct was created from the pBabe p53DD (…PromoterAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+Lux
Plasmid#26160DepositorInsertG13 promoter + Bacterial luciferase operon
UseTagsExpressionBacterialMutationIt contains a gram-positive enhanced translation …PromoterAvailable sinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minCMV
Plasmid#211518PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-AMOT p130
Plasmid#32821DepositorInsertAMOT p130 (AMOT Human)
UseTags2xHAExpressionMammalianMutationE5DPromoterCMVAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ALK
Plasmid#23917DepositorInsertALK (ALK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCfB2191
Plasmid#67538PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG198 PRKD1 3'UTR
Plasmid#12010DepositorInsertPRKD1 3'UTR (PRKD1 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
mVenus-IQ6 R6A
Plasmid#198202PurposeFRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)DepositorInsertMYO5A IQ domain (Myo5a Mouse)
UseTagsmVenusExpressionMammalianMutationR6APromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
UseTagsExpressionMammalianMutationA206K bothPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL1P4 ZmUbiP:GRF-GIF:NosT
Plasmid#198048PurposeMoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.InsertZmUbiP::TaGRF4-GIF1::NosT
UseSynthetic BiologyTagsExpressionMutationPromoterZea mays (maize) ubiquitin promoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hACE2-hygro
Plasmid#161758PurposeExpresses human ACE2 in mammalian cellsDepositorInsertHuman ACE2 (ACE2 Human)
UseLentiviralTagsP2AExpressionMammalianMutationPromoterEF1aAvailable sinceNov. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
EMTB-3XGFP
Plasmid#26741DepositorInsertensconsin (MAP7 Human)
UseProbe for microtubulesTags3x EGFP and MycExpressionMammalianMutationOnly contains the microtubule binding-domain of e…PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_MCART1
Plasmid#140591PurposeExpress MCART1 in S. cerevisiaeDepositorInsertSLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
UseTagsExpressionYeastMutationcodon-optimized for expression in S. cerevisiaePromoterNDT1 promoter and 3'UTRAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
B*57:03:01
Plasmid#203260PurposeOverexpress HLA alleleDepositorInsertB*57:03:01 (HLA-B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL003
Plasmid#215335PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterpSpankAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL07
Plasmid#104431Purposemitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertmito-Super Ecliptic pHluorin-mRuby2
UseTagsExpressionYeastMutationPromoterTef1Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDLxR6
Plasmid#172560PurposeInducible gene expression, pCDF origin, LuxR activator, PLuxB promoter, mRFP, Streptomycin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLuxBAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA#2
Plasmid#64246Purposeexpresses sgRNA under U6a promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
S96 ELP
Plasmid#68393PurposeElastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residueDepositorInsertS96 ELP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-His7tag-Peredox-mCherry
Plasmid#32382DepositorInsertHis7-Peredox-mCherry
UseTagsHis7tagExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cre
Plasmid#198281PurposeExpresses Cre in mammalian cellsDepositorInsertCre
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Halo-KDEL
Plasmid#124316PurposeExpresses ER specific HaloTag proteinDepositorInsertHalo tag
UseTagsKDEL SeqExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-9-5p
Plasmid#103749PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-9-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-9-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG_mRuby2_smFP OLLAS
Plasmid#59761Purposeencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)DepositorInsertmRuby2_smFP_OLLAS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only