171,852 results

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pAS1-rab-3P::his-24::GCaMP6s

Plasmid

#124351

Purpose

Nuclear Localized Pan-Neuronal GCaMP6s

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

rab-3, GCaMP6s (rab-3 Nematode)

Expression

Worm

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRRG227-Wnt1

Plasmid

#99073

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Wnt1 (also known as WntP-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9

Plasmid

#49330

Purpose

Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Plasmid

#205162

Purpose

express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

scFv-GCN4-HAtag-msfGFP(V206K)-GBI

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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ptagBFP-LacI-NLS-VP16

Plasmid

#103837

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI-NLS-VP16

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-TetON-Sox10

Plasmid

#115242

Purpose

Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

SOX10 (SOX10 Human)

Use

Lentiviral

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-tdTomato-WPRE

Plasmid

#51506

Purpose

Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV5

Insert

tdTomato

Use

AAV

Promoter

phSyn1

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pRRG203-notum

Plasmid

#99067

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea notum

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

notum in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168 delta MIU2

Plasmid

#133979

Purpose

mammalian expression vector of Flag tagged RNF168 where the second motif interacting with ubiquitin (MIU) has been deleted

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Mutation

deletion of MIU2

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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PD13

Plasmid

#164530

Purpose

Expresses HA-Ub noK

Depositor

Gregory Pazour

Article

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Ubiquitin noK

Use

Lentiviral

Tags

HA and Ub noK

Mutation

All seven Lysines of Ubiquitin changed to Arginine

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p3869 HA-E6-AP C833A

Plasmid

#8649

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Mutation

C833A; active site Cys mutated to encode Ala

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_Hygro(+)_GRHL1-L3F6H

Plasmid

#177765

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1

Depositor

Michael Ristow

Article

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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LBR EGFP

Plasmid

#128150

Purpose

Expression of lamin B receptor

Depositor

Jennifer Lippincott-Schwartz

Article

Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

Insert

lamin B receptor (LBR Human)

Expression

Mammalian

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-NLS-RNF168 delta RING

Plasmid

#133983

Purpose

mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

eGFP

Expression

Mammalian

Mutation

deletion of RING; siRNA resistant sequence: GAGGA…

Promoter

CMV

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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p70a-T7RNAP

Plasmid

#105683

Purpose

Expresses T7 polymerase in cell-free TXTL.

Depositor

Chase Beisel

Article

Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

Insert

T7 polymerase

Expression

Bacterial

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-6XHis-SUMO-SIRT2

Plasmid

#102622

Purpose

Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Tags

6XHis-SUMO

Expression

Bacterial

Mutation

Deleted amino acids 1-37 and 357-389

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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T7-AglC-His6

Plasmid

#89726

Purpose

Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglC

Tags

His6 and T7

Expression

Bacterial

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Nicn1

Plasmid

#92344

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived Alu element containing 3'UTR (NICN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-2xFYVE

Plasmid

#140047

Purpose

Expresses GFP-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available Since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

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pTG612

Plasmid

#237387

Purpose

mStayGold^NSEC^ repair template

Depositor

Ariel Pani

Article

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mStayGold^NSEC^

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pTG613

Plasmid

#237388

Purpose

mStayGold::AID^NSEC^ repair template

Depositor

Ariel Pani

Article

Gibney et al Genetics. 2026 Jan 20:iyag016. doi: 10.1

Insert

mStayGold::AID^NSEC^

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAL750 :: msfGFP

Plasmid

#250661

Purpose

Inducible promoter Pxyl expressing msfGFP in haloarcheon Haloferax volcanii

Depositor

Alexandre Bisson

Article

Rados et al Front Microbiol. 2023 Aug 10;14:1204876.

Insert

Monomeric Superfolder Green Fluorescent Protein

Use

Haloarchaeal expression

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-P2A-H2A-tdiRFP (JDW 1402)

Plasmid

#242591

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an H2A-tdiRFP for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pTCas9_scr

Plasmid

#207566

Purpose

ThermoCas9-mediated cleavage of genomic DNA in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pICSL12013

Plasmid

#245630

Purpose

Level 0 Golden Gate part 6GAL4UAS promoter, GGAG - AATG

Depositor

Mark Youles

Insert

6GAL4UAS promoter

Use

Synthetic Biology

Mutation

BsaI/BpiI sites removed by point-mutation

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI (JDW 1494)

Plasmid

#242592

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mScarlet3-S2 (JDW 1514)

Plasmid

#242552

Purpose

Gateway middle entry clone containing H2B-mScarlet3-S2; Nuclear red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-S2

Use

Gateway subcloning

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iATPSnFR2.A95K.tdTomato

Plasmid

#244921

Purpose

iATPSnFR2.A95K variant with non-responsive tdTomato

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.tdTomato

Use

AAV

Mutation

A95K

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-Pho8∆60-HphMX6

Plasmid

#240973

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-hCA4-IV77

Plasmid

#244906

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-IV77

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(mem).iGlucoSnFR2

Plasmid

#244088

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.GFAP.(cyto).iGlucoSnFR2

Plasmid

#244111

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
NanoBiT Assay Vector, HER1(1-668)-LgBiT

Plasmid

#237104

Purpose

Express HER1(1-668)-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

HER1 (EGFR Human)

Tags

LgBiT

Expression

Mammalian

Mutation

1-668

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAAV-Rat SARE-Arc Min Promoter-Flag Arc-SV40 3'UTR

Plasmid

#233057

Purpose

To express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains a SV40 3' UTR

Depositor

Jonathan Ploski

Insert

Rat Arc (Arc Rat)

Use

AAV

Tags

Flag

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-USP7-sh1

Plasmid

#235527

Purpose

shRNA against human USP7

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

USP7 (USP7 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-USP7-sh5

Plasmid

#235528

Purpose

shRNA against human USP7

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

USP7 (USP7 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
RNF168 C-terminal sgRNA

Plasmid

#207100

Purpose

pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GAGATGCACAAAGTAAGGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK2_Rluc-Y0x-Fluc

Plasmid

#233614

Purpose

Expression of Rluc-Fluc reporter gene containing no Y

Depositor

Shintaro Iwasaki

Article

Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

Insert

Rluc-Y0x-Fluc

Use

Luciferase

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SeqCBE

Plasmid

#232115

Purpose

Expresses AncBE4max, nSeqCas9 (D10A), and puromycin resistance genes

Depositor

Yongming Wang

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-rab-3P::AI::gur-3G::SL2::his-24::tagBFP

Plasmid

#232608

Purpose

Pan-neuronal expression of 'GUR-3' using a genomic fragment and fluorophore 'tagBFP', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

GUR-3 (gur-3 Nematode)

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-5xQUAS+Δpes-10P::AI::eTsChR

Plasmid

#232612

Purpose

Expression of opsin 'eTsChR' on activation via 'QF+hGR' chimeric protein

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

eTsChR

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_FL Gcn4

Plasmid

#231855

Purpose

Bacterial expression of N-terminally 6His tagged FL Gcn4

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Aquamarine-LC3B G120A-TdLanYFP

Plasmid

#228561

Purpose

Encodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoter

Depositor

Giulia Bertolin

Article

Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

Aquamarine and TdLanYFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHelper-CRISPRi-sgRNA

Plasmid

#221135

Purpose

Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

sgRNA-scaffold with dCas9 handle

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI)

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB-CRISPRi

Plasmid

#221136

Purpose

Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

3xF-dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Bacterial

Promoter

cbu1169 promoter

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-H2B-V5-n2StayGoldC4 (JDW 1354)

Plasmid

#224486

Purpose

Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-V5-n2StayGoldC4

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Leu2 2u

Plasmid

#220622

Purpose

empty backbone for shuttle vector construction

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Insert

empty vector (LEU2 Budding Yeast)

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-6xHis-GFP(0)-L1

Plasmid

#205033

Purpose

Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(0)-L1

Tags

GRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Mutation

E16R, I138R, D207R, N222K, L246R

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-myc-EphB4

Plasmid

#200983

Purpose

Mammalian expression plasmid for myc-tagged EphB4

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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