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178,238 results

Showing: 10201 - 10250 of 178238 results

  3. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

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  5. pAD-GamS

    Plasmid
    #99246
    Purpose
    Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    gamS (with C-terminal 6xHis tag)
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99246-plasmid-map-sequence-id-339990

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  17. pcDNA5-MTS-TagBFP-P2AT2A-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87829
    Purpose
    High-efficient mammalian expression vector for co-expression of BFP-, EGFP- and mCherry-tagged proteins using P2AT2A. MTS, NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    MTS
    NLS
    PTS1
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87829-plasmid-map-sequence-id-175007

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  19. pCMV-SPORT6-hHMGCR1

    Plasmid
    #86085
    Purpose
    expresses the human HMG-CoA reductase isoform 1
    Depositor
    Anne Galy
    Insert
    human HMG-CoA Reductase isoform 1 (HMGCR Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only

  24. p5E-gfap

    Plasmid
    #75024
    Purpose
    Can be used to drive expression in zebrafish astrocytes.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    glial fibrillary acidic protein promoter (gfap Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    gfap
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75024-plasmid-map-sequence-id-450679

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  30. PMXS-SLC1A3

    Plasmid
    #72873
    Purpose
    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    SLC1A3 (SLC1A3 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only

  36. pETM6-E12-vioABECD

    Plasmid
    #66537
    Purpose
    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)
    Depositor
    Mattheos Koffas
    Article
    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10
    Inserts
    VioA
    VioB
    VioE
    VioC
    VioD
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …
    Available Since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    66537-plasmid-map-sequence-id-114568

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  40. pJZC116

    Plasmid
    #62344
    Purpose
    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 2x MS2 (wt+f6) binding module
    MCP-VP64
    Use
    Lentiviral
    Tags
    VP64
    Expression
    Mammalian
    Mutation
    Targets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGC
    Promoter
    CMV and U6
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62344-plasmid-map-sequence-id-102267

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  41. pMF440

    Plasmid
    #62550
    Purpose
    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.
    Depositor
    Michael Franklin
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    trc
    Available Since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    62550-plasmid-map-sequence-id-397042

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  42. pVITRO1-Trastuzumab-IgA1/κ

    Plasmid
    #61888
    Purpose
    Expresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)
    Depositor
    Andrew Beavil
    Article
    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103
    Inserts
    HER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    Expression
    Mammalian
    Promoter
    mEF1 Prom and rEF1 Prom
    Available Since
    March 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61888-plasmid-map-sequence-id-101180

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  43. PX854

    Plasmid
    #62886
    Purpose
    C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Insert
    SpCas9 (aa573-1368)
    Use
    CRISPR
    Tags
    FKBP12, NLS SV40, and NLS nucleoplasmin
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62886-plasmid-map-sequence-id-105909

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  50. FKBP_SCF2_LD6_NTEV

    Plasmid
    #58878
    Purpose
    MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58878-plasmid-map-sequence-id-94239

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Showing: 10201 - 10250 of 178238 results