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175,644 results

Showing: 1301 - 1350 of 175644 results

  2. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

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  5. pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV5)

    Viral Prep
    #105677-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    Syn
    Tags
    FusionRed (Cre-dependent)
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  7. pAAV-Ef1a-fDIO EYFP (AAV9)

    Viral Prep
    #55641-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available Since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-Syn-ChrimsonR-tdT (AAV5)

    Viral Prep
    #59171-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. pGP-AAV-syn-jGCaMP7s-WPRE (AAV1)

    Viral Prep
    #104487-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP7s-WPRE (#104487). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid
    #71294
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    GNSTM glycosylation motif, HA, Lamp2 signal pepti…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71294-plasmid-map-sequence-id-418480

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  15. pLV-EGFP-Cre

    Plasmid
    #86805
    Purpose
    Lentiviral vector that expresses an EGFP-Cre fusion protein with a nuclear localization signal from the CMV promoter
    Depositor
    Amy Lasek
    Article
    Truitt et al eNeuro. 2016 Oct 31;3(5). pii: ENEURO.02
    Insert
    Cre recombinase
    Use
    Cre/Lox and Lentiviral
    Tags
    EGFP and nuclear localization signal: PKKKRKV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    86805-plasmid-map-sequence-id-436570

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  16. CRISPRi GuEST-List GFP v1

    Pooled Library
    #248189
    Purpose
    The CRISPRi Guest for Enrichment-based Screening of Targets Library (CRISPRi GuEST-List v1) enables genome-wide, single-cell pooled screens conducted with the Flex workflow from 10x Genomics.
    Depositor
    Rahul Satija
    Article
    Bradu et al bioRxiv 2026.02.12.705613
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    CRISPR and Lentiviral
    Available Since
    Feb. 17, 2026
    Availability
    Academic Institutions and Nonprofits only

  20. pHN924

    Plasmid
    #178885
    Purpose
    Expression of GST-His8-HRV3C
    Depositor
    Kazuhiro Abe
    Insert
    human rhinovirus 3C protease
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    178885-plasmid-map-sequence-id-354971

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  22. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  23. pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)

    Viral Prep
    #162378-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (#162378). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8m-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    Feb. 14, 2022
    Availability
    Academic Institutions and Nonprofits only

  24. pXPR_047

    Plasmid
    #107145
    Purpose
    measures Cas9 activity
    Depositor
    David Root
    Insert
    GFP + sgRNA for GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107145-plasmid-map-sequence-id-205295

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  25. pAAV-hSyn-hM4D(Gi)-mCherry (AAV8)

    Viral Prep
    #50475-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-hM4D(Gi)-mCherry (#50475). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM4D(Gi)-mCherry plasmid DNA. hSyn-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only

  28. JD171

    Plasmid
    #229483
    Purpose
    Adenoviral helper plasmid containing E2A, VA RNA I and L4-22k/33k genes
    Depositor
    Nerea Zabaleta
    Article
    Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3
    Insert
    E2A, VA RNA I, L4-22k/33k
    Use
    AAV and Adenoviral
    Expression
    Mammalian
    Promoter
    CMV (E2A), Ef1a (L4-22k/33k) and wt promoter (VA …
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    229483-plasmid-map-sequence-id-455009

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  29. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV9)

    Viral Prep
    #26975-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  35. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (AAV PHP.eB)

    Viral Prep
    #105540-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. Expression of EGFP-Cre from hSyn promoter. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    EGFP
    Available Since
    Sept. 4, 2018
    Availability
    Academic Institutions and Nonprofits only

  37. mCherry2-C1

    Plasmid
    #54563
    Purpose
    Localization: C1 Cloning Vector, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    mCherry2
    Expression
    Mammalian
    Available Since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54563-plasmid-map-sequence-id-310151

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  48. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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Showing: 1301 - 1350 of 175644 results