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179,463 results

Showing: 1401 - 1450 of 179463 results

  2. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12 Articles
    47948-plasmid-map-sequence-id-68812

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  5. pCI-VSVG

    Plasmid
    #1733
    Purpose
    VSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan lab
    Depositor
    Garry Nolan
    Insert
    VSV-G Envelope
    Expression
    Mammalian
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    41 Articles
    1733-plasmid-map-sequence-id-519303

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  6. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (AAV1)

    Viral prep
    #162377-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP8s-WPRE (#162377). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP8s-WPRE plasmid DNA. Syn-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Publication
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-hSyn-hM3D(Gq)-mCherry (AAV Retrograde)

    Viral prep
    #50474-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only

  17. pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV8)

    Viral prep
    #154867-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ulrik Gether
    Publication
    Runegaard et al eNeuro. 2018 Jun 18;5(3). pii: eN-NWR-03
    Promoter
    Human Synapsin-1
    Tags
    mCherry (Flp-dependent)
    Available since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only

  18. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid
    #103871
    Purpose
    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    103871-plasmid-map-sequence-id-259670

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  19. pLenti puro HA-Ubiquitin

    Plasmid
    #74218
    Purpose
    Lentiviral expression of HA tagged Ubiquitin
    Depositor
    Melina Fan
    Insert
    ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available since
    March 14, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    16 Articles
    74218-plasmid-map-sequence-id-148715

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  26. pLenti-MetGFP

    Plasmid
    #37560
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 13, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    22 Articles
    37560-plasmid-map-sequence-id-339875

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  27. pAAV-FLEX-GFP

    Plasmid
    #28304
    Purpose
    Cre-dependent AAV-mediated expression of GFP under the CAG promoter
    Depositor
    Edward Boyden
    Insert
    GFP
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CAG
    Available since
    July 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47 Articles
    28304-plasmid-map-sequence-id-450290

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  31. pLB

    Plasmid
    #11619
    Purpose
    pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.
    Depositor
    Stephan Kissler
    Publication
    Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    mouse U6
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    30 Articles
    11619-plasmid-map-sequence-id-274493

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  35. pAAV-hSyn Con/Fon EYFP (AAV8)

    Viral prep
    #55650-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn Con/Fon EYFP (#55650). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon EYFP plasmid DNA. Synapsin driven, Cre and Flp-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  36. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV1)

    Viral prep
    #20297-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  41. pAAV-Ef1a-DIO EYFP

    Plasmid
    #27056
    Depositor
    Karl Deisseroth
    Has service
    AAV1, AAV2, AAV5, AAV9, and AAV Retrograde
    Insert
    EYFP
    Use
    AAV
    Expression
    Mammalian
    Available since
    Jan. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    203 Articles
    27056-plasmid-map-sequence-id-126562

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  44. pAAV/D377Y-mPCSK9

    Plasmid
    #58376
    Purpose
    Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis
    Depositor
    Jacob Bentzon
    Publication
    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do
    Has service
    AAV8
    Insert
    proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    D377Y
    Promoter
    HCRApoE/hAAT
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    33 Articles
    58376-plasmid-map-sequence-id-292482

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Showing: 1401 - 1450 of 179463 results